Substrate Utilization by Embryogenic and Non-Embryogenic Cell Suspension Cultures of Cucumis sativus L.

Callebaut, Alfons; Motte, J. C.; Cat, W. De

doi:10.1016/s0176-1617(87)80146-3

Cited by 17 publications

(6 citation statements)

References 18 publications

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“…Callebaut et al (5) recently reported that a nonembryogenic suspension culture of cucumber exhibited the same preferential uptake of glucose as demonstrated in this work. In contrast, its embryogenic counterpart utilized both glucose and fructose at equal rates.…”

supporting

confidence: 86%

Substrate Utilization by Suspension Cultures and Somatic Embryos of Daucus carota L. Measured by ¹³C NMR

Dijkema¹,

Vries²,

Booij³

et al. 1988

Plant Physiol.

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The uptake and utilization of sucrose by embryogenic suspension cultures of carrot (Daucus carota L.) growing in the presence of 2,4-D and by somatic embryos derived from these cultures was monitored using (13)C nuclear magnetic resonance. The exogeneously supplied sucrose was completely hydrolyzed before cell entry; glucose was taken up preferentially when the cells were cultured in the presence of 2,4-D, while glucose and fructose were utilized at similar rates by somatic embryos in the absence of 2,4-D. Both suspension cells and somatic embryos accumulated high intracellular levels predominantly of glucose and sucrose, the latter being resynthesized intracellularly from the constitutive hexoses. Initially, fructose was converted mainly into glucose and sucrose rather than being catabolized directly through glycolysis or the pentose phosphate pathway. Carbohydrate supply that exceeded cellular demand resulted in intracellular accumulation of mono- or disaccharides. The capacity of cultured carrot cells to produce somatic embryos appeared to be positively correlated with high intracellular levels of glucose.

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supporting

confidence: 86%

Substrate Utilization by Suspension Cultures and Somatic Embryos of Daucus carota L. Measured by ¹³C NMR

Dijkema¹,

Vries²,

Booij³

et al. 1988

Plant Physiol.

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“…However, fructose and galactose were the best for embryo development. Callebaut et al (1987) reported that embryogenic and nonembryogenic cucumber cell cultures utilized sugars in different patterns, which might be based on specific sugar carriers.…”

Section: Methodsmentioning

confidence: 99%

“…Although systematic screenings of genotypes are rare, there is no doubt that genetic factors play a major role in somatic embryogenesis competence (Jelaska 1974;Malepszy & Nadolska-Orczyk 1983;Callebaut et al 1987;Colijn-Hooymans et al 1988a, b;Gray et al 1993;Oridate et al 1992). Nadolska- suggested a genetic determinism in the ability to regenerate somatic embryo-derived plants from cucumber (Cucumis sativus L.) leaf explants: the 'intermediately' and 'intensively' regenerating lines contained two pairs of dominant genes responsible for in vitro plant regeneration, characterized by complementary and probably additive interactions.…”

Section: Genotypementioning

confidence: 99%

Somatic embryogenesis in Cucurbitaceae

Debeaujon

Branchard²

1993

Plant Cell Tiss Organ Cult

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Strategies based on the application of biotechnologies to crop improvement programmes generally require regeneration of whole plants from cells or tissues cultivated in vitro. In Cucurbitaceae, regeneration can occur either through a caulogenic or an embryogenic developmental pathway. Reports of somatic embryogenesis have dealt with the main cultivated crops, i.e. cucumber, melon, squash and watermelon. Somatic embryogenesis and plant recovery are obtained from numerous sources including protoplasts, but the best results are observed with explants coming from seedlings, especially cotyledons and hypocotyls. The genetic constitution of mother plants also seems to play a key role in the success of embryogenesis, but few systematic studies on genotype effect have been published. Somatic embryos can exhibit developmental abnormalities, particularly when they arise from protoplast-derived cultures. Generally, data concerning embryo yield, rate of germination and plant development and characteristics of regenerated plants and their progeny, has not been provided in previous reports. The potential use of somatic embryogenesis in cucurbit breeding programmes is stressed in this review.

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“…Embryogenic cell suspension cultures have been initiated from primary explants by an indirect pathway via the callus stages (Malepszy and Solarek 1986;Callebaut et al 1987;Chee and Tricoli 1988;Bergervoet et al 1989;Raharjo and Punja 1994;Schulze et al 1995;Vengadesan et al 2005), or by a direct pathway in liquid medium from the vegetative shoot apices (Wróblewski et al 1995) and from radicles of germinated cucumber seeds (Kreuger et al 1996;Pellinen et al 1997). The main limitation of cucumber embryogenesis has, until now, been the high frequency of abnormal embryos formation combined with a low frequency of plant regeneration (Cade et al 1990;Debeaujon and Branchard 1993;Wróblewski et al 1995;Pellinen et al 1997;Łady_ zyński et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Long-term suspension cultures of cucumber (Cucumis sativus L.) with high embryogenic potential

et al. 2009

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Cucumber (Cucumis sativus L.) cytokininindependent embryogenic cell suspension cultures were derived and maintained for more than 3.5 years without losing the embryogenic potential. The preparation and the characteristics of the cucumber embryogenic cell suspension possess many similarities to that of carrot. The cultures were induced from hypocotyl explants of in vitro grown cucumber plants in liquid MS media containing 2,4-dichlorophenoxyacetic acid as the sole growth regulator during 6 weeks and they contained a heterogeneous array of several different types of single cells and cell clusters (PEMs). The established cell suspensions were subcultured in 1-week interval, while the inoculation density was optimized to 2.0 9 10 5 cells ml -1 using cell viability as a marker. Somatic embryos were obtained after the transfer of the proembryogenic masses to a hormone-free semisolid MS medium with a frequency of 388 ± 57 somatic embryos per 1 ml of packed cell volume of the established cucumber embryogenic culture within 7 days. The frequency of normal somatic embryos with two cotyledons was found to be 78%. Such embryos possessed the potential of spontaneous maturation and the embryo conversion rates were 87%. The yield of normally growing plants was much higher compared with that previously described for cucumber systems. Somatic embryo-derived plants were successfully transferred to the greenhouse where they flowered and fruited.

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Substrate Utilization by Embryogenic and Non-Embryogenic Cell Suspension Cultures of Cucumis sativus L.

Cited by 17 publications

References 18 publications

Substrate Utilization by Suspension Cultures and Somatic Embryos of Daucus carota L. Measured by ¹³C NMR

Substrate Utilization by Suspension Cultures and Somatic Embryos of Daucus carota L. Measured by ¹³C NMR

Somatic embryogenesis in Cucurbitaceae

Long-term suspension cultures of cucumber (Cucumis sativus L.) with high embryogenic potential

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Substrate Utilization by Embryogenic and Non-Embryogenic Cell Suspension Cultures of Cucumis sativus L.

Cited by 17 publications

References 18 publications

Substrate Utilization by Suspension Cultures and Somatic Embryos of Daucus carota L. Measured by 13C NMR

Substrate Utilization by Suspension Cultures and Somatic Embryos of Daucus carota L. Measured by 13C NMR

Somatic embryogenesis in Cucurbitaceae

Long-term suspension cultures of cucumber (Cucumis sativus L.) with high embryogenic potential

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Product

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Substrate Utilization by Suspension Cultures and Somatic Embryos of Daucus carota L. Measured by ¹³C NMR

Substrate Utilization by Suspension Cultures and Somatic Embryos of Daucus carota L. Measured by ¹³C NMR