Subtle Differences in Virus Composition Affect Disinfection Kinetics and Mechanisms

Sigstam, Thérèse; Gannon, Greg; Cascella, M.; Pecson, Brian M.; Wigginton, Krista; Kohn, Tamar

doi:10.1128/aem.00663-13

Cited by 80 publications

(98 citation statements)

References 43 publications

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“…Minor differences in structure and genetic composition are known to influence virus inactivation by different disinfectants (17,31). Here the observed susceptibility to RS was greatest for MS2, intermediate for EV, and lowest for HAdV and X174 (with approximately similar susceptibilities).…”

Section: Fig 3 Inactivation Kinetics Of Bacteriophages Ms2 (A) and X1mentioning

confidence: 77%

Solar Disinfection of Viruses in Polyethylene Terephthalate Bottles

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Mattle

et al. 2016

Appl Environ Microbiol

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b Solar disinfection (SODIS) of drinking water in polyethylene terephthalate (PET) bottles is a simple, efficient point-of-use technique for the inactivation of many bacterial pathogens. In contrast, the efficiency of SODIS against viruses is not well known. In this work, we studied the inactivation of bacteriophages (MS2 and X174) and human viruses (echovirus 11 and adenovirus type 2) by SODIS. We conducted experiments in PET bottles exposed to (simulated) sunlight at different temperatures (15, 22, 26, and 40°C) and in water sources of diverse compositions and origins (India and Switzerland). Good inactivation of MS2 (>6-log inactivation after exposure to a total fluence of 1.34 kJ/cm 2 ) was achieved in Swiss tap water at 22°C, while less-efficient inactivation was observed in Indian waters and for echovirus (1.5-log inactivation at the same fluence). The DNA viruses studied, X174 and adenovirus, were resistant to SODIS, and the inactivation observed was equivalent to that occurring in the dark. High temperatures enhanced MS2 inactivation substantially; at 40°C, 3-log inactivation was achieved in Swiss tap water after exposure to a fluence of only 0.18 kJ/cm 2 . Overall, our findings demonstrate that SODIS may reduce the load of single-stranded RNA (ssRNA) viruses, such as echoviruses, particularly at high temperatures and in photoreactive matrices. In contrast, complementary measures may be needed to ensure efficient inactivation during SODIS of DNA viruses resistant to oxidation.

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Section: Fig 3 Inactivation Kinetics Of Bacteriophages Ms2 (A) and X1mentioning

confidence: 77%

Solar Disinfection of Viruses in Polyethylene Terephthalate Bottles

Carratalà

Calado

Mattle

et al. 2016

Appl Environ Microbiol

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“…Because thiosulfate can back-reduce oxidized protein residues, disinfection experiments in which protein integrity was assessed were conducted by the one-time addition of ClO 2 , which was then left to evaporate as was described before (Sigstam et al, 2013). All MALDI measurements were performed with an ABI 4800 MALDI-TOF-TOF (Applied Biosystems, Rotkreuz, Switzerland), using the instrument settings and sample deposition methods described previously (Wigginton et al, 2010).…”

Section: Analysis Of the Capsid Protein By Matrix Assisted Laser Desomentioning

confidence: 99%

“…As described in previous work (Sigstam et al, 2013), chlorine dioxide inactivation kinetics of MS2 deviate from the firstorder ChickeWatson model and can be described by the Hom model according to the following equation (Haas and Joffe, 1994):…”

Section: Analysis Of Disinfection Kineticsmentioning

confidence: 99%

“…The parameters for the Hom model were fitted in Sigmaplot (version: 12.0, 2011). Model fits were compared by ANCOVA analysis as described previously (Sigstam et al, 2013). The correlation coefficient (R) for all fits varied between 0.97 and 0.99.…”

Section: Analysis Of Disinfection Kineticsmentioning

confidence: 99%

“…1, however, we demonstrate that ClO 2 consumption is not the sole reason for tailing, as this feature is evident even though the chlorine dioxide concentration in our experiments was maintained constant. Interestingly, no tailing effect was observed for the inactivation of MS2 by other oxidants, such as free chlorine, peracetic acid or singlet oxygen (Mattle et al, 2011;Sigstam et al, 2013;Wigginton et al, 2012). The tailing feature must therefore be related to the specific mode of action of ClO 2 .…”

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confidence: 93%

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On the cause of the tailing phenomenon during virus disinfection by chlorine dioxide

et al. 2014

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TailingReversible protection MALDI Resistant subpopulation a b s t r a c tThis study investigates the mechanisms underlying the deviation from ChickeWatson kinetics, namely a tailing curve, during the disinfection of viruses by chlorine dioxide (ClO 2 ). Tailing has been previously reported, but is typically attributed to the decay in disinfectant concentration. Herein, it is shown that tailing occurs even at constant ClO 2 concentrations. Four working hypothesis to explain the cause of tailing were tested, specifically changes in the solution's disinfecting capacity, aggregation of viruses, resistant virus subpopulations, and changes in the virus properties during disinfection. In experiments using MS2 as a model virus, it was possible to rule out the solution's disinfecting capacity, virus aggregation and the resistant subpopulation as reasons for tailing. Instead, the cause for tailing is the deposition of an adduct onto the virus capsid over the course of the experiment, which protects the viruses. This adduct could easily be removed by washing, which restored the susceptibility of the viruses to ClO 2 . This finding highlights an important shortcoming of ClO 2 , namely its self-limiting effect on virus disinfection. It is important to take this effect into account in treatment applications to ensure that the water is sufficiently disinfected before human consumption. ª 2013 Elsevier Ltd. All rights reserved. IntroductionChlorination is among the oldest and most commonly used disinfection process worldwide. However, over the years it has been shown that chlorine produces harmful by-products such as trihalomethanes and other halogenated compounds with potential carcinogenic effects (Xie, 2004). It is therefore of interest to investigate other disinfectants that have a similar disinfection potential but generate fewer problematic byproducts. As a good alternative, chlorine dioxide (ClO 2 ) has shown to efficiently disinfect water for human consumption (Huang et al., 1997;Jin et al., 2013;Zoni et al., 2007).Importantly, it is effective at inactivating Cryptosporidium, whereas free chlorine is not (Chauret et al., 2001). Except from exhibiting a good disinfection capacity, ClO 2 can also oxidize iron and manganese, as well as help controlling taste and odor compounds (Aieta and Berg, 1986;Li et al., 1996). The disadvantage of using chlorine dioxide is that it reacts to chlorite, which may be neurotoxic at high doses (Xie, 2004). In 1908, Chick published the first model for describing bacteria inactivation by disinfecting agents (Chick, 1908) Available online at www.sciencedirect.com journal homepage: www.else vier.com/locate /wa tres w a t e r r e s e a r c h 4 8 ( 2 0 1 4 ) 8 2 e8 90043-1354/$ e see front matter ª

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Inactivation of Pathogenic Microorganisms on Polymeric Materials through Ozonation

et al. 2023

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Ozone-based technologies have been evaluated to inactivate the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on surfaces. However, the vast diversity of information makes it difficult to establish common ground for determining the best practices for using this technology. The objective of this work is to evaluate the success of N95 mask decontamination by ozonation, determining the specific parameters for process control. To quantify the effectiveness of the process, a disinfection protocol was initially developed based on two bacterial species (Escherichia coli and Staphylococcus pseudintermedius), followed by another disinfection assay using the murine hepatitis coronavirus (MHV-3), in a laboratory-scale prototype. Ozone is an effective candidate for use against SARS-CoV-2 or other viruses to disinfect personal protection equipment (PPE).

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Subtle Differences in Virus Composition Affect Disinfection Kinetics and Mechanisms

Cited by 80 publications

References 43 publications

Solar Disinfection of Viruses in Polyethylene Terephthalate Bottles

Solar Disinfection of Viruses in Polyethylene Terephthalate Bottles

On the cause of the tailing phenomenon during virus disinfection by chlorine dioxide

Inactivation of Pathogenic Microorganisms on Polymeric Materials through Ozonation

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