2024
DOI: 10.1101/2024.03.04.583254
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Subtomogram averaging of helical filaments in cryo-electron tomography

Xiaojie Zhang,
Julia Mahamid

Abstract: Helical filaments are essential macromolecular elements in cellular organization and dynamics. Recent advances in cryo-electron tomography allow faithful imaging of isolated or in-cell helical filaments. Here, we present a protocol to generate density maps at sub-nanometer resolution of helical filaments by subtomogram averaging, exemplified with isolated mumps virus nucleocapsids and their in-cell form as an extension of the method. We detail procedures from pre-processing of tilt series frames to refinement … Show more

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