Subtractive proteomics to identify novel drug targets and reverse vaccinology for the development of chimeric vaccine against Acinetobacter baumannii

Solanki, Vandana; Tiwari, Vishvanath

doi:10.1038/s41598-018-26689-7

Cited by 222 publications

(169 citation statements)

References 78 publications

Supporting

Mentioning

169

Contrasting

Order By: Relevance

“…The optimized DNA sequences were taken and SgrA1 and SphI restriction sites were conjugated at the N-terminal and C-terminal sites, respectively. Next, the SnapGene [73] restriction cloning module was used to insert the new adapted DNA sequences between SgrA1 and SphI of pET-19b vector.…”

Section: Codon Adaptation and In Silico Cloningmentioning

confidence: 99%

Designing Novel Subunit Vaccines against Herpes Simplex Virus-1 using Reverse Vaccinology Approach

Sarkar

Ullah

2020

Preprint

View full text Add to dashboard Cite

Herpes Simplex Virus (HSV) is an infectious virus that is responsible for various types of orofacial and genital infections. Two types of HSV viruses, HSV-1 and HSV-2, are the most dangerous HSV viruses. Every year, millions of people get infected with this menacing virus, however, no satisfactory treatment or vaccine has not yet been discovered to fight against HSV. Although there are some anti-viral therapies, however, studies have showed that such anti-viral therapies may also fail to provide good impact. In this study, a possible subunit vaccine against HSV-1, strain-17, was designed using the tools and reverse vaccinology and bioinformatics. Three potential antigenic envelope glycoproteins were selected from nine envelope glycoproteins, for possible vaccine construction. Potential epitopes capable of inducing high immunogenic response and at the same time have non-allergenicity and conservancy across other strains and species, were selected by some robust analysis, for vaccine construction. Finally, three possible vaccines were designed.Each of the vaccine construct differ from each other only in their adjuvant sequences and based on molecular docking analysis, one best vaccine construct was selected for molecular dynamics simulation study and in silico codon adaptation. The experiment showed that the selected best vaccine should be good candidate against HPV-1, strain-17. However, wet lab study should be conducted on the suggested vaccine(s) for confirming their potentiality, safety and efficacy.

show abstract

Section: Codon Adaptation and In Silico Cloningmentioning

confidence: 99%

Designing Novel Subunit Vaccines against Herpes Simplex Virus-1 using Reverse Vaccinology Approach

Sarkar

Ullah

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…BglI and BglII), prokaryote ribosome-binding site and Rho independent transcription termination were kept away from the work (Grote et al, 2005).Than mRNA sequence of constructed V1 vaccine was conjugated with BglI and BglII restriction site at the C-terminal and N-terminal sites respectively. In this case, SnapGene was used for the cloning purpose (Solanki & Tiwari, 2018).…”

Section: Codon Adaptation and In Silico Cloningmentioning

confidence: 99%

Scrutinizing surface glycoproteins and poxin-schlafen protein to design a heterologous recombinant vaccine against monkeypox virus

Mni

et al. 2020

Preprint

View full text Add to dashboard Cite

Monkeypox is a zoonotic disease caused by monkeypox virus with noteworthy mortality and morbidity. Several recent outbreaks and the need of dependable reconnaissance have raised the level of concern for this developing zoonosis. In the present study, a reverse vaccinology strategy was developed to construct a peptide vaccine against monkeypox virus by exploring cell surface binding protein, Poxin-Schlafen andenvelope protein. Both humoral and cell mediated immunity induction were the main concerned properties for the designed peptide vaccine. Therefore, both T cell and B cell immunity against monkeypox virus were analyzed from the conserver region of the selected protein. Antigenicity testing, transmembrane topology screening, allergenicity and toxicity assessment, population coverage analysis and molecular docking approach were used to create the superior epitopes of moneypox virus. The subunit vaccine was constructed using highly immunogenic epitopes with appropriate adjuvant and linkers. Molecular docking examination of the refined vaccine with various MHCs and human immune receptor illustrated higher binding interaction. The designed construct was reverse transcribed and adjusted for E. coli strain K12 earlier to inclusion inside pET28a(+) vector for its heterologous cloning and expression. The study could start in vitro and in vivo studies concerning effective vaccine development against monkeypox virus.

show abstract

“…DbD2, an online tool was used to design such bonds for the constructed vaccine protein [97]. Inflammations caused by bacterial antigen are involved with TLR-2 immune receptors present over the immune cells [98][99][100]. An approach for protein-protein docking was employed to determine the binding affinity of designed subunit vaccines with different HLA alleles and TLR-2 immune receptor by using ClusPro [101], hdoc [102,103] and PatchDock server [104].…”

Section: Vaccine Tertiary Structure Prediction Disulfide Engineeringmentioning

confidence: 99%

Proteome Exploration of Legionella pneumophila for Identifying Novel Therapeutics: A Hierarchical Subtractive Genomics and Reverse Vaccinology Approach

Khan

Mahmud

Hasan

et al. 2020

Preprint

View full text Add to dashboard Cite

Legionella pneumophila, the causative agent of a serious type of pneumonia (lung infection) called Legionnaires' disease. It is emerging as an antibacterial resistant strain day by day.Hence, identification of novel drug targets and vaccine candidates is essential to fight against this pathogen. Herein attempts were taken through subtractive genomics approach on complete proteome of L. pneumophila to address the challenges of multidrug resistance. A total 2930 proteins from L. pneumophila proteome were investigated through diverse subtractive proteomics approaches, e.g., identification of human non homologous and pathogen specific essential proteins, druggability and 'anti-target' analysis, prediction of subcellular localization, human microbiome non-homology screening, protein-protein interactions studies in order to find out effective drug and vaccine targets. Only 3 were identified that fulfilled all these criteria and proposed as novel drug targets against L. pneumophila. Furthermore, outer membrane protein TolB was identified as potential vaccine target with better antigenicity score and allowed for further in silico analysis to design a unique multiepitope subunit vaccine against it. Antigenicity and transmembrane topology screening, allergenicity and toxicity assessment, population coverage analysis and molecular docking approach were adopted to generate the most potent epitopes. The final vaccine was constructed by the combination of highly immunogenic epitopes along with suitable adjuvant and linkers. The designed vaccine construct showed higher binding interaction with different MHC molecules and human immune TLR2 receptor with minimum deformability at molecular level. The translational potency and microbial expression of the vaccine protein was also analyzed using pET28a(+) vector. The present study aids in the development of novel therapeutics and vaccine candidates for efficient treatment of the infections caused by Legionella pneumophila. However, further wet lab based investigations and in vivo trials are highly recommended to experimentally validate our prediction.

show abstract

Subtractive proteomics to identify novel drug targets and reverse vaccinology for the development of chimeric vaccine against Acinetobacter baumannii

Cited by 222 publications

References 78 publications

Designing Novel Subunit Vaccines against Herpes Simplex Virus-1 using Reverse Vaccinology Approach

Designing Novel Subunit Vaccines against Herpes Simplex Virus-1 using Reverse Vaccinology Approach

Scrutinizing surface glycoproteins and poxin-schlafen protein to design a heterologous recombinant vaccine against monkeypox virus

Proteome Exploration of Legionella pneumophila for Identifying Novel Therapeutics: A Hierarchical Subtractive Genomics and Reverse Vaccinology Approach

Contact Info

Product

Resources

About