Subtype ayw variant of hepatitis B virus

Bichko, V.; Pushko, Peter; Dreilina, Dzidra; Pumpen, P.; Gren, E.J.

doi:10.1016/0014-5793(85)80771-7

Cited by 151 publications

(89 citation statements)

References 15 publications

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“…Recombinant HBcAg (subtype ayw) encompassing residues 1-183 and with an Ile at position 80 and a Gly at position 74 was designated HBcAg-Ile (Bichko et al, 1985). Full-length mutants of HBcAg-Ile lacking the regions 79-85 (HBcD79-85) and 76-85 (HBcD76-85), respectively, were expressed and purified from E. coli.…”

Section: Methodsmentioning

confidence: 99%

Priming of cytotoxic T cell responses to exogenous hepatitis B virus core antigen is B cell dependent

Lazdina

Alheim

Nyström

et al. 2003

Journal of General Virology

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The hepatitis B virus (HBV) core antigen (HBcAg) has a unique ability to bind a high frequency of naive human and murine B cells. The role of HBcAg-binding naive B cells in the immunogenicity of HBcAg is not clear. The HBcAg-binding properties of naive B cells were characterized using HBcAg particles with mutated spike region (residues 76-85) sequences. Deletion of residues 76-85 (HBcD76-85) destroyed naive B cell binding, whereas deletion of residues 79-85 did not. HBcAg particles with an Ile instead of the natural Ala at position 80 did not bind naive B cells, whereas reversion of Ile 80 RAla restored B cell binding. Destroying the B cell-binding ability of HBcAg had a marginal effect on the overall B cell immunogenicity of the different particles, suggesting that they were equally efficient in priming T helper cells. Therefore, the importance of HBcAg-binding B cells is studied with relation to the priming of HBcAg-specific cytotoxic T cells (CTLs). The role of HBcAg-binding B cells in the priming of HBcAg-specific CTLs was evaluated by immunization with endogenous HBcAg (DNA immunization) and exogenous recombinant HBcAg particles. Endogenous HBcAg primed HBcAg-specific CTLs in wild-type and B cell-deficient mice, whereas exogenous HBcAg primed HBcAg-specific CTLs only in wild-type mice. Importantly, HBcD76-85 did not prime CTLs despite the presence of B cells. Thus, the ability of exogenous HBcAg particles to prime specific CTLs is B cell dependent, suggesting a possible role for HBcAgbinding B cells in HBV infections.

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Section: Methodsmentioning

confidence: 99%

Priming of cytotoxic T cell responses to exogenous hepatitis B virus core antigen is B cell dependent

Lazdina

Alheim

Nyström

et al. 2003

Journal of General Virology

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“…The MAbs 14Ell, 14G3, 14E12, 13D9, 13B1, 14D5, 10F10, 13C9, 10C6 and 13D3 were raised against SDS-denaturated rHBcAg (drHBcAg of ayw subtype; Bichko et al, 1985Bichko et al, , 1992, which also was used for the screening of primary hybridomas. These will be referred to as MAbs against drHBcAg in the following text.…”

Section: Methodsmentioning

confidence: 99%

Immunochemical structure of the carboxy-terminal part of hepatitis B e antigen: identification of internal and surface-exposed sequences

Sällberg¹,

Pushko

Berzinsh

et al. 1993

Journal of General Virology

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“…1); clone 5, pIDW420 (Fig. 1); clone 6, pRTB299 (Sastrosoewignjo et al, 1985); clone 7, pSK619 (Okamoto et al, 1987a); clone 8, pAK66; clone 9, pIWK146; clone 10, pHBVI-1 (Kobayashi & Koike, 1984); clone 11, pHBr330 (Ono etal., 1983); clone 12, pBRHBadr4 (Fujiyama etal., 1983); clone 13, pNDR260; clone 14, pYRB259 ; clone 15, Eco HBV DNA (Galibert et al, 1979); clone 16, pHBV320 (Bichko et al, 1985); clone 17, pYWB796; clone 18, pPYW310. The sequences for clones 8, 9, 13, 17 and 18, as well as their proposed genetic organizations (unpublished data) are available upon request from the authors.…”

Section: -D 18mentioning

confidence: 99%

Typing Hepatitis B Virus by Homology in Nucleotide Sequence: Comparison of Surface Antigen Subtypes

Okamoto

Tsuda

Sakugawa

et al. 1988

Journal of General Virology

950

774

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SUMMARYThe complete nucleotide sequences of the DNA of three hepatitis B virus (HBV) genomes of subtype adw, cloned from plasma samples of asymptomatic carriers living in the mainland and Okinawa Prefecture of Japan and Indonesia were determined. All three comprised 3215 bp and differed in sequence by only 3.9 to 5.6%. When these isolates were compared with the reported sequences of two HBV genomes of the same subtype derived from American carriers, however, the differences were greater (8.3 to 9.3%) to an extent comparable with the nucleotide divergence between an HBV genome of subtype adw and that of a heterotypic subtype, such as adr, ayw or ayr. A total of 18 HBV genomes of various subtypes, including the three described here, 10 reported previously and five unpublished ones, were classified into four groups based on an inter-group divergence in nucleotide sequence of 8 ~o or greater: group A (two adw genomes), group B (four adw), group C (three adw, four adr and one ayr) and group D (four ayw). Thus, the nine genomes of HBV subtype adw were distributed into three groups with considerably different sequences. These results indicate that the four major antigenically defined subtypes of envelope polypeptide do not reflect true genotypic variation of HBV. The fact that dtoy, as well as w to r, subtypic change can be induced by an A --, G point mutation at nucleotides 365 and 479 in the S gene, respectively, supports this view.

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Subtype ayw variant of hepatitis B virus

Cited by 151 publications

References 15 publications

Priming of cytotoxic T cell responses to exogenous hepatitis B virus core antigen is B cell dependent

Priming of cytotoxic T cell responses to exogenous hepatitis B virus core antigen is B cell dependent

Immunochemical structure of the carboxy-terminal part of hepatitis B e antigen: identification of internal and surface-exposed sequences

Typing Hepatitis B Virus by Homology in Nucleotide Sequence: Comparison of Surface Antigen Subtypes

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