Subtype‐dependent postnatal development of taste receptor cells in mouse fungiform taste buds

Ohtubo, Yoshitaka; Iwamoto, Masafumi; Yoshii, Kentaro

doi:10.1111/j.1460-9568.2012.08068.x

Cited by 18 publications

(26 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the number of receptor cells as defined by PLCβ2 and Car4 staining increases proportionately as the taste bud grows. This result is consistent with a previous study in which IP 3 R3 cells (another taste receptor cell marker) increase in parallel with the total number of taste bud cells during postnatal development (Ohtubo et al, 2012). However, the proportion of Sox2+/Keratin-8- cells decreases during development.…”

Section: Discussionsupporting

confidence: 93%

“…Remodeling is not required for neurons to make initial connections with taste buds. However, following initial innervation, taste buds continue to grow and differentiate postnatally (Bigiani et al, 2002; Hosley and Oakley, 1987; Kinnamon et al, 2005; Ohtubo et al, 2012; Zhang et al, 2008), resulting in an adult taste bud that has multiple different cell types based on anatomy, function, and expression (Clapp et al, 2006; Clapp et al, 2004; Delay et al, 1986; Finger, 2005; Kataoka et al, 2008; Murray and Murray, 1967; Murray et al, 1969; Yang et al, 2000a; Yang et al, 2000b; Yee et al, 2001). Therefore, it would not be surprising if some developmental remodeling were required for taste receptor cells to be innervated by the appropriate nerve fibers.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Postnatal reduction of BDNF regulates the developmental remodeling of taste bud innervation

Huang

Krimm

2015

Developmental Biology

View full text Add to dashboard Cite

The refinement of innervation is a common developmental mechanism that serves to increase the specificity of connections following initial innervation. In the peripheral gustatory system, the extent to which innervation is refined and how refinement might be regulated is unclear. The initial innervation of taste buds is controlled by brain-derived neurotrophic factor (BDNF). Following initial innervation, taste receptor cells are added and become newly innervated. The connections between the taste receptor cells and nerve fibers are likely to be specific in order to retain peripheral coding mechanisms. Here, we explored the possibility that the down-regulation of BDNF regulates the refinement of taste bud innervation during postnatal development. An analysis of BDNF expression in BdnflacZ/+ mice and real-time reverse transcription polymerase chain reaction (RT-PCR) revealed that BDNF was down-regulated between postnatal day (P) 5 and P10. This reduction in BDNF expression was due to a loss of precursor/progenitor cells that express BDNF, while the expression of BDNF in the subpopulations of taste receptor cells did not change. Gustatory innervation, which was identified by P2X3 immunohistochemistry, was lost around the perimeter where most progenitor/precursor cells are located. In addition, the density of innervation in the taste bud was reduced between P5 and P10, because taste buds increase in size without increasing innervation. This reduction of innervation density was blocked by the overexpression of BDNF in the precursor/progenitor population of taste bud cells. Together these findings indicate that the process of BDNF restriction to a subpopulation of taste receptor cells between P5 and P10, results in a refinement of gustatory innervation. We speculate that this refinement results in an increased specificity of connections between neurons and taste receptor cells during development.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Postnatal reduction of BDNF regulates the developmental remodeling of taste bud innervation

Huang

Krimm

2015

Developmental Biology

View full text Add to dashboard Cite

show abstract

“…In fungiform papillae, small numbers of Type II and III cells are present at birth, with the majority of taste cells differentiating postnatally (Ohtubo et al, 2012;Zhang et al, 2006Zhang et al, , 2007. Although we did not detect Type III cells at E18.5, a small proportion of fungiform papillae contained Type II cells and at least a third possessed Type I cells, indicating that taste cell differentiation begins late in embryonic development.…”

Section: Stabilization Of β-Catenin Within Shhmentioning

confidence: 61%

ß-Catenin signaling regulates temporally discrete phases of anterior taste bud development

Thirumangalathu

Barlow

2015

Development

View full text Add to dashboard Cite

The sense of taste is mediated by multicellular taste buds located within taste papillae on the tongue. In mice, individual taste buds reside in fungiform papillae, which develop at mid-gestation as epithelial placodes in the anterior tongue. Taste placodes comprise taste bud precursor cells, which express the secreted factor sonic hedgehog (Shh) and give rise to taste bud cells that differentiate around birth. We showed previously that epithelial activation of β-catenin is the primary inductive signal for taste placode formation, followed by taste papilla morphogenesis and taste bud differentiation, but the degree to which these later elements were direct or indirect consequences of β-catenin signaling was not explored. Here, we define discrete spatiotemporal functions of β-catenin in fungiform taste bud development. Specifically, we show that early epithelial activation of β-catenin, before taste placodes form, diverts lingual epithelial cells from a taste bud fate. By contrast, β-catenin activation a day later within Shh + placodes, expands taste bud precursors directly, but enlarges papillae indirectly. Further, placodal activation of β-catenin drives precocious differentiation of Type I glial-like taste cells, but not other taste cell types. Later activation of β-catenin within Shh + precursors during papilla morphogenesis also expands taste bud precursors and accelerates Type I cell differentiation, but papilla size is no longer enhanced. Finally, although Shh regulates taste placode patterning, we find that it is dispensable for the accelerated Type I cell differentiation induced by β-catenin.

show abstract

“…No nucleus was immunoreactive to IP 3 R3 or SNAP‐25. These confocal images were similar to previous ones (Yang et al ., ; Ohtubo & Yoshii, ; Ohtubo et al ., ). We identified 14 type II cells, nine type III cells and 18 non‐IRCs.…”

Section: Resultsmentioning

confidence: 97%

Cell‐type‐dependent action potentials and voltage‐gated currents in mouse fungiform taste buds

Kimura

Ohtubo

Tateno

et al. 2013

Eur J of Neuroscience

Self Cite

View full text Add to dashboard Cite

Taste receptor cells fire action potentials in response to taste substances to trigger non-exocytotic neurotransmitter release in type II cells and exocytotic release in type III cells. We investigated possible differences between these action potentials fired by mouse taste receptor cells using in situ whole-cell recordings, and subsequently we identified their cell types immunologically with cell-type markers, an IP3 receptor (IP3 R3) for type II cells and a SNARE protein (SNAP-25) for type III cells. Cells not immunoreactive to these antibodies were examined as non-IRCs. Here, we show that type II cells and type III cells fire action potentials using different ionic mechanisms, and that non-IRCs also fire action potentials with either of the ionic mechanisms. The width of action potentials was significantly narrower and their afterhyperpolarization was deeper in type III cells than in type II cells. Na(+) current density was similar in type II cells and type III cells, but it was significantly smaller in non-IRCs than in the others. Although outwardly rectifying current density was similar between type II cells and type III cells, tetraethylammonium (TEA) preferentially suppressed the density in type III cells and the majority of non-IRCs. Our mathematical model revealed that the shape of action potentials depended on the ratio of TEA-sensitive current density and TEA-insensitive current one. The action potentials of type II cells and type III cells under physiological conditions are discussed.

show abstract

Subtype‐dependent postnatal development of taste receptor cells in mouse fungiform taste buds

Cited by 18 publications

References 45 publications

Postnatal reduction of BDNF regulates the developmental remodeling of taste bud innervation

Postnatal reduction of BDNF regulates the developmental remodeling of taste bud innervation

ß-Catenin signaling regulates temporally discrete phases of anterior taste bud development

Cell‐type‐dependent action potentials and voltage‐gated currents in mouse fungiform taste buds

Contact Info

Product

Resources

About