Subtyping of Magnetically Isolated Breast Cancer Cells Using Magnetic Force Microscopy

Abstract

Circulating tumor cell (CTC) which recently arisen as potential sources for monitoring and characterizing non-haematologic cancers and their metastatic derivatives. Immunomagnetic microbeads and magnetic nanoparticles (MNPs) have been extensively explored to isolate CTCs from blood samples. However, MNPs attached on the membrane protein are interrupted further analysis to distinguish the cancer subtype by consumption or blocking the target surface marker. Here, an MNP-mediated analysis method for surface marker expression profile by magnetic force microscopy (MFM) is described. Two MNPs, zinc ferrite and iron oxide, are showed distinct phase shift (-16.5° and -3.7°, respectively) signal on the MFM images. The antibody conjugated MNPs are successfully isolated target cells without giving damage to the cell. The MFM image of MNP decorated cells show clear differences between two breast cancer cell lines, MCF-7 and SK-BR-3, which proof the cancer subtyping property using MFM method. To confirmation of the surface marker consumption during the cell isolation, antibody-conjugated quantum dots and drug-loaded oleosome are treated on the cells, thereby MNP decorated cells are survived. This newly developed MFM analysis method provides a new direction to utilize the MNP for the surface marker expression phenotypes.

Microfluidic Chip-Based Cancer Diagnosis and Prediction of Relapse by Detecting Circulating Tumor Cells and Circulating Cancer Stem Cells

“…To date, diverse platforms have been developed to efficiently separate CTCs and CCSCs. Among them, immunomagnetic separation by specific antibody-functionalized magnetic nanoparticles is the most frequently used strategy [34]. This technique offers several advantages, owing to the fast magnetic response, high surface area, and good biocompatibility of magnetic nanoparticles.…”

Recent Advancements of Specific Functionalized Surfaces of Magnetic Nano- and Microparticles as a Theranostics Source in Biomedicine

“…By the use of conventional (coprecipitation, sol–gel, microemulsion) and advanced (hydrothermal, template synthesis) synthesis routes, various materials such as polymers, precious metals, and silica have been produced and evaluated as MNMP coatings, which address the above-mentioned requirements. Such coatings can entrap bioactive molecules, − e.g. , curcuma and DNA and RNA .…”

Indirect magnetic force microscopy

“…5 For superparamagnetic nanoparticles, MFM has been especially useful to evaluate magnetic moment, 6,7 magnetic anisotropy, 8 magnetization curves 9 and the effect of aggregation 10,11 in particles. Applications of MFM have also extended to evaluate biological samples 12 such as ferritin proteins, 13,14 cells labeled with magnetic particles, 15 and magnetic (iron) deposits in bacteria 16,17 and in mammalian tissues. 18,19…”