Subtyping of ST22-MRSA-IV (Barnim epidemic MRSA strain) at a university clinic in Germany from 2002 to 2005

Ghebremedhin, Beniam; König, Wolfgang; Witte, Wolfgang; Hardy, Katherine; Hawkey, Peter M.; König, B.

doi:10.1099/jmm.0.46883-0

Cited by 25 publications

(31 citation statements)

References 46 publications

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“…The SIRU method's typeability, i.e., the method's ability to assign a type to all isolates tested, was 89.4% when new primers designed for SIRU05 and SIRU16 locus amplification were used. The consistent nonamplification of SIRU05 from all strains belonging to ST1, CC22, CC45, and CC80 observed with our collection when we used the previously published SIRU05 primers is in agreement not only with previous studies focusing on SIRU typing of EMRSA-15 (CC22) isolates (29,(35)(36)(37) but also with the work of Ikawaty et al, who reported a reduced typeability for this specific locus on CC1, CC5, CC8, CC97, and CC228 isolates as well (39).…”

Section: Discussionsupporting

confidence: 80%

“…This allelic profile makes the SIRU method amenable to interlaboratory comparisons and database management, comparable to MLST. So far, the SIRU method has been applied to S. aureus isolates from nosocomial outbreaks in the United Kingdom and Germany, mainly MRSA isolates, and therefore to highly related strains (29,(35)(36)(37). Very recently, a single study evaluated a MLVA scheme including the SIRU typing loci and the sspA gene, using a European collection of contemporary S. aureus isolates (39).…”

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confidence: 99%

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Staphylococcal Interspersed Repeat Unit Typing of Staphylococcus aureus : Evaluation of a New Multilocus Variable-Number Tandem-Repeat Analysis Typing Method

2009

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The present study evaluates the performance of the staphylococcal interspersed repeat unit (SIRU) method applied to a diverse collection of 104 Staphylococcus aureus isolates previously characterized by pulsed-field gel electrophoresis (PFGE), spa typing, multilocus sequence typing (MLST), and staphylococcal cassette chromosome mec typing for methicillin-resistant S. aureus. The SIRU method distributed the 104 strains into 81 SIRU profiles that could be clustered into 12 groups and 29 singletons. The discriminatory power of the method at the profile level, translated by Simpson's index of diversity (SID), was similar to that of PFGE subtyping (SID ‫؍‬ 99.23% versus 99.85%) and slightly higher than that of spa typing (SID ‫؍‬ 97.61%). At the group level, the SIRU SID (93.24%) was lower than that of PFGE typing (95.41%) but higher than that of MLST (SID ‫؍‬ 91.77%). The adjusted Rand (AR) coefficient showed that SIRU typing at the group level had the highest congruence with MLST (AR ‫؍‬ 0.5736) and with clonal complex (CC) (AR ‫؍‬ 0.4963) but the lowest congruence with PFGE subtype (AR ‫؍‬ 0.0242). The Wallace coefficient indicated that in the present collection, two strains with the same SIRU profile have a 100% probability of belonging to the same CC, a 90% probability of sharing the same spa type, and an 83% probability of being classified in the same sequence type. The high discriminatory power of the SIRU method, along with its apparent concordance with MLST results, makes it potentially valuable for S. aureus short-term epidemiological investigations and population dynamics as well.

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Section: Discussionsupporting

confidence: 80%

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confidence: 99%

Staphylococcal Interspersed Repeat Unit Typing of Staphylococcus aureus : Evaluation of a New Multilocus Variable-Number Tandem-Repeat Analysis Typing Method

2009

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“…Within the German cohort, the MRSA prevalence was relatively low (n=2/100) which reflects earlier dataset of the University Clinic in Magdeburg. All MRSA and MSSA isolates were negative for PVL gene [11][12][13].…”

Section: Discussionmentioning

confidence: 99%

“…Overall, the study data of the two cohorts indicate geographical differences among nasal colonization by staphylococci, including MSSA and MRSA, and other Gram-positive and -negative bacterial species. S. aureus colonization was less common in Eritrean pediatric cohort; in contrast, the occurrence of this species including low number of methicillin resistant isolates was observed more frequently in the German cohort which corresponds to the local epidemiologic prevalence and clonality of MRSA in the study region [11][12][13]. Controlled, prospective clinical trials in the future might be helpful to determine whether nasal carriage of Gramnegative bacteria in such patients lead to the incidence of infection with these bacterial organisms.…”

Section: Proteus Mirabilis 1 -mentioning

confidence: 99%

“…After morphological and microscopic examination, relevant bacteria were further identified by VITEK2 ® . Antimicrobial susceptibility results were evaluated by using the Clinical and Laboratory Standards Institute (CLSI) reference methods [10], and polymerase chain reaction (PCR) assays for detection of PVL gene in S. aureus was performed as previously [11,12].…”

Section: Microbiological Studymentioning

confidence: 99%

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Comparative study of nasal bacterial carriage in pediatric patients from two different geographical regions

Ghebremedhin

Köenig

2012

European Journal of Microbiology and Immunology

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The aim of this study was to determine the prevalence of bacterial carriage in the anterior nares of two different patient cohorts, aged 5-15 years. By use of a sensitive enrichment broth, Gram-positive and -negative bacteria were cultured from the two cohorts of each 100 patients at the Referral Clinic in Eritrea and at a German University Hospital. In the German cohort, 27% of the patients were positive either for Gram-negative (n=5) or -positive bacteria, including Staphylococcus aureus (n=8; MRSA (n=2)), Staphylococcus epidermidis (n=12), Corynebacterium spp. (n=4), and Streptococcus pyogenes (n=1). In comparison, the Eritrean cohort revealed 33% bacterial carriers in the anterior nares. Among the identified species were S. aureus (n=2), S. epidermidis (n=13), Streptococcus haemolyticus (n=9), and Gram-negative rods including Klebsiella pneumoniae/oxytoca (n=5), Enterobacter agglomerans (n=4), Escherichia coli (n=2), and Pseudomonas aeruginosa (n=1). Noteworthy, none of the Eritrean patients were positive for MRSA. In both cohorts there was no co-occurrence of Gram-positive and -negative bacteria in the anterior nares. However, we observed in two subjects of the Eritrean cohort co-colonization with S. epidermidis and S. haemolyticus. The occurrence of Gram-negative bacteria was less significant by age in the German cohort, whereas in the Eritrean cohort Gram-negative bacteria were more frequently detected in carriers aged 5-9 years. Continued surveillance of S. aureus and Gram-negative bacteria carriage deserves further attention and might help to determine future trends in the characteristics of nasal carriage, subsequent incidence of infections, and the potential effectiveness of targeted population based intervention.

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On-line resources for bacterial micro-evolution studies using MLVA or CRISPR typing

et al. 2008

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Subtyping of ST22-MRSA-IV (Barnim epidemic MRSA strain) at a university clinic in Germany from 2002 to 2005

Cited by 25 publications

References 46 publications

Staphylococcal Interspersed Repeat Unit Typing of Staphylococcus aureus : Evaluation of a New Multilocus Variable-Number Tandem-Repeat Analysis Typing Method

Staphylococcal Interspersed Repeat Unit Typing of Staphylococcus aureus : Evaluation of a New Multilocus Variable-Number Tandem-Repeat Analysis Typing Method

Comparative study of nasal bacterial carriage in pediatric patients from two different geographical regions

On-line resources for bacterial micro-evolution studies using MLVA or CRISPR typing

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