Subvisual chromatin changes in cervical epithelium measured by texture image analysis and correlated with HPV

Automated analysis of immunohistochemistry images identifies candidate location biomarkers for cancers

“…Most such studies use visual examination to assess changes, a difficult and time-consuming task. With the advent of high-throughput acquisition technologies like tissue microarrays and automated slide scanners, computerized analysis of tissue images is highly desirable, and studies have shown that quantitative software can detect changes in disease states that are missed by visual inspection (7). Methods for analyzing changes in expression and pattern are well established in cultured cells (8), but histological images are typically more difficult to analyze because cellular heterogeneity and the closely packed organization of cells lead to significant cell segmentation challenges.…”

Human Papillomavirus and Related Diseases - From Bench to Bedside - A Clinical Perspective

“…The distinction of florid reactive changes, immature metaplastic patterns, and atrophic changes from HPVinduced alterations may cause difficulties. Attempts have been made to redefine the traditional criteria for lesion diagnosis, while other efforts aimed at the adoption of new, more objective methods, which might support the former (Bollmann et al, 2005;Cho et al, 2005;Guillaud et al, 2005;Prasad et al, 1994;Salvia et al, 2004;Scheurer et al, 2007). However, studies attempting to correlate HPV presence and replication to certain cytohistologic alterations are becoming less frequent and/or fruitful.…”

Immunohistochemistry in the Diagnosis of Squamous Intraepithelial Lesions of the Uterine Cervix

“…The distinction of florid reactive changes, immature metaplastic patterns, and atrophic changes from HPVinduced alterations may cause difficulties. Att e m p t s h a v e b e e n m a d e t o r e d e f i n e t h e traditional criteria for lesion diagnosis, while other efforts aimed at the adoption of new, more objective methods, which might support the former (Bollmann et al, 2005;Cho et al, 2005;Guillaud et al, 2005;Prasad et al, 1994;Salvia et al, 2004;Scheurer et al, 2007). However, studies attempting to correlate HPV presence and replication to certain cytohistologic alterations are becoming less frequent and/or fruitful.…”