Subzero 12-hour Nonfreezing Cryopreservation of Porcine Heart in a Variable Magnetic Field

Seguchi, Ryuta; Watanabe, Go; Kato, Hiroki; Yamaguchi, S

doi:10.1097/txd.0000000000000544

Cited by 10 publications

(4 citation statements)

References 28 publications

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“…There were no significant morphological, immunohistochemical, or immunofluorescent changes observed under a light microscope at I-15, I-30, or I-60 min without reperfusion. Via TEM, the mitochondria of the I-60 min without reperfusion conformed with Flameng 13 2–3 points, whereas those of the I-60 min with R-2 h conformed with Flameng 3–4 points. We were able to observe evident structural changes in the mitochondria at I-15 (Flameng 2 points).…”

Section: Resultsmentioning

confidence: 90%

Mitochondrial miR-23b-5p is a new biomarker of warm ischemic injury in donor livers and a candidate for graft evaluation: experimental studies

Kong¹,

Zhou²,

Yuan³

et al. 2023

International Journal of Surgery

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Background and aims: Warm ischaemic injury (WII) stems from incorrect energy metabolism and is the main cause of graft dysfunction. Mitochondria, as the centre of cellular metabolic activities, may be the key in identifying accurate indicators for evaluating the quality of grafts. Our research focuses on the screening, clinical application, and mechanism of the optimal WII mitochondrion biomarker. Approach and results: Using a 100% hepatic warm ischaemia mouse model, without reperfusion, transmission electron microscopy demonstrated evident morphological changes of hepatic mitochondria at 15 min of ischaemia. However, all 13 mt-mRNAs could not display continuously upregulated consistency at 0–15–30–60 min during WII. High-throughput analysis of miRNA expression in both purified mitochondria and liver tissues suggested miR-23b-5p was a potential mitochondrial microRNA (mitomiR) biomarker with high sensitivity and 0-15-30-60 min change consistency. Fluorescence in-situ hybridization and reverse transcription quantitative polymerase chain reaction (RT-qPCR) further confirmed the results. Through overexpression and inhibition, the functionality of this mitomiR during WII was identified as a protective regulator in vitro and then verified in Dicer1 fl/fl Alb Cre mice by downregulation of other miRNAs and supplementation of mature mitomiR-23b-5p. Dual-luciferase reporter assay and the Seahorse XF analyzer determined that mitomiR-23b-5p reduced mitochondrial respiratory function by silencing mt-RNR2 (16S). Clinically, mitomiR-23b-5p was positively correlated with serum alanine aminotransferase levels 3 days after the operation ( P =0.032), and the C-statistic for 90-day graft survival rate was 0.698. Conclusions: MitomiR-23b-5p plays a protective regulatory role and implements a special mitochondrial regulation mechanism not yet reported in WII. These clinical results further support the experimental result that the expression of MitomiR-23b-5p is closely related to the prognosis of clinical liver transplantation patients. This is a promising new biomarker for WII evaluation of donor livers.

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Section: Resultsmentioning

confidence: 90%

Mitochondrial miR-23b-5p is a new biomarker of warm ischemic injury in donor livers and a candidate for graft evaluation: experimental studies

Kong¹,

Zhou²,

Yuan³

et al. 2023

International Journal of Surgery

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“…This technique results in a significant reduction of anerobic metabolism and myocardial protection in the heart and is thus used for the preservation the of heart. [ 21 ]…”

Section: Different Modes Of Cryopreservationmentioning

confidence: 99%

Cryopreservation: A Comprehensive Overview, Challenges, and Future Perspectives

et al. 2023

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Cryopreservation is the most prevalent method of long‐term cell preservation. Effective cell cryopreservation depends on freezing, adequate storage, and correct thawing techniques. Recent advances in cryopreservation techniques minimize the cellular damage which occurs while processing samples. This article focuses on the fundamentals of cryopreservation techniques and how they can be implemented in a variety of clinical settings. The article presents a brief description of each of the standard cryopreservation procedures, such as slow freezing and vitrification. Alongside that, the membrane permeating and nonpermeating cryoprotectants are briefly discussed, along with current advancements in the field of cryopreservation and variables influencing the cryopreservation process. The diminution of cryoinjury incurred by the cell via the resuscitation process will also be highlighted. In the end application of cryopreservation techniques in many fields, with a special emphasis on stem cell preservation techniques and current advancements presented. Furthermore, the challenges while implementing cryopreservation and the futuristic scope of the fields are illustrated herein. The content of this review sheds light on various ways to enhance the output of the cell preservation process and minimize cryoinjury while improving cell revival.

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“…The Flameng score, an index of ultrastructural changes in mitochondria, was significantly lower in the subzero group than in the conventional group (1.28 ± 0.40 vs. 2.73 ± 0.30; P < 0.05). Altogether, subzero ice-free preservation using a variable magnetic field suppressed anaerobic metabolism and was associated with myocardial protection in both rat and porcine hearts ( 49 ). However, future studies would need to show the effect of the magnetic field and its benefit compared to CPAs or isochoric freezing, as no comparative analyses were carried out in the presented studies.…”

Section: Supercooling Principles To Extend Organ Preservationmentioning

confidence: 99%

Supercooling: a promising technique for prolonged preservation in solid organ transplantation, and early perspectives in vascularized composite allografts

Berkane,

Hayau,

Filz von Reiterdank

et al. 2023

Front. Transplant.

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Ex vivo preservation of transplanted organs is undergoing spectacular advances. Machine perfusion is now used in common practice for abdominal and thoracic organ transportation and preservation, and early results are in favor of substantially improved outcomes. It is based on decreasing ischemia-reperfusion phenomena by providing physiological or sub-physiological conditions until transplantation. Alternatively, supercooling techniques involving static preservation at negative temperatures while avoiding ice formation have shown encouraging results in solid organs. Here, the rationale is to decrease the organ's metabolism and need for oxygen and nutrients, allowing for extended preservation durations. The aim of this work is to review all advances of supercooling in transplantation, browsing the literature for each organ. A specific objective was also to study the initial evidence, the prospects, and potential applications of supercooling preservation in Vascularized Composite Allotransplantation (VCA). This complex entity needs a substantial effort to improve long-term outcomes, marked by chronic rejection. Improving preservation techniques is critical to ensure the favorable evolution of VCAs, and supercooling techniques could greatly participate in these advances.

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Subzero 12-hour Nonfreezing Cryopreservation of Porcine Heart in a Variable Magnetic Field

Abstract: Subzero nonfreezing preservation using a variable magnetic field resulted in a remarkable suppression of anaerobic metabolism and myocardial protection in porcine hearts.

Cited by 10 publications

References 28 publications

Mitochondrial miR-23b-5p is a new biomarker of warm ischemic injury in donor livers and a candidate for graft evaluation: experimental studies

Mitochondrial miR-23b-5p is a new biomarker of warm ischemic injury in donor livers and a candidate for graft evaluation: experimental studies

Cryopreservation: A Comprehensive Overview, Challenges, and Future Perspectives

Supercooling: a promising technique for prolonged preservation in solid organ transplantation, and early perspectives in vascularized composite allografts

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