Subzero 24-hr Nonfreezing Rat Heart Preservation

Kato, Hiroki; Tomita, Shigeyuki; Yamaguchi, Sumie; Ohtake, Hiroshi; Watanabe, Go

doi:10.1097/tp.0b013e3182637054

Cited by 26 publications

(8 citation statements)

References 16 publications

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“…To enhance the current quality of donor hearts, researchers continue to experiment with various methods in animals. For example, improvement of the UW solution18, a two-layer method using UW19, and super cooling with UW20 have all been attempted. These attempts have resulted in the stock solution having organ- and/or cell-protective effects because immersion in a stock solution is generally considered important to organ preservation1011.…”

Section: Discussionmentioning

confidence: 99%

Functional evaluation of rat hearts transplanted after preservation in a high-pressure gaseous mixture of carbon monoxide and oxygen

Hatayama

Inubushi

Naito

et al. 2016

Sci Rep

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We recently succeeded in resuscitating an extracted rat heart following 24–48 hours of preservation in a high-pressure gaseous mixture of carbon monoxide (CO) and oxygen (O2). This study aimed to examine the function of rat hearts transplanted after being preserved in the high-pressure CO and O2 gas mixture. The hearts of donor rats were preserved in a chamber filled with CO and O2 under high pressure for 24 h (CO24h) or 48 h at 4 °C. For the positive control (PC) group, hearts immediately extracted from donor rats were used for transplantation. The preserved hearts were transplanted into recipient rats by heterotopic cervical heart transplantation. CO toxicity does not affect the grafts or the recipients. Light microscopy and [18F]-fluorodeoxyglucose positron emission tomography revealed that there were no significant differences in the size of the myocardial infarction or apoptosis of myocardial cells in post-transplant hearts between the PC and CO24h groups. Furthermore, at 100 days after the transplantation, the heart rate, weight and histological staining of the post-transplanted hearts did not differ significantly between the PC and CO24h groups. These results indicate that the function of rat hearts is well preserved after 24 hours of high-pressure preservation in a CO and O2 gas mixture. Therefore, high-pressure preservation in a gas mixture can be a useful method for organ preservation.

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Section: Discussionmentioning

confidence: 99%

Functional evaluation of rat hearts transplanted after preservation in a high-pressure gaseous mixture of carbon monoxide and oxygen

Hatayama

Inubushi

Naito

et al. 2016

Sci Rep

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“…Kato et al . [19] reported that rat hearts preserved in a variable magnetic field at -3°C for 24 hours exhibited satisfactory hemodynamic and metabolic performance. Preservation as described above only reduces the metabolic needs of tissues, and is thus not suitable for long-term storage.…”

Section: Discussionmentioning

confidence: 99%

“…Modern cryobiological theory holds that the most dangerous temperature zone during cryopreservation is 0 to -60°C, especially 0 to -10°C, because intercellular ice accumulates at these temperatures and cell damage occurs within this temperature zone during the process of cooling or thawing [3,19,27]. Moreover, it has been reported that thawing too slowly induces recrystallization of the intercellular ice, which results in fatal injury to the cell [28].…”

Section: Discussionmentioning

confidence: 99%

“…Fasciotomy would not likely have prevented the compromised blood flow as the replanted muscles were only loosely sutured. In our prior study using a rat model of limb cryopreservation, transmission electron microscopic observation of muscle that had been cryopreserved and thawed showed that mitochondrial swelling was present within the muscle fibers, most muscle fibers were disorganized, glycogen granules were reduced, the number of mitochondrial cristae were decreased, the myofilaments became loose, the z-line became obscured or disappeared, and autolysis of myeloid bodies occurred [19]. …”

Section: Discussionmentioning

confidence: 99%

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Cryopreservation and replantation of amputated rat hind limbs

Wang

Duan

et al. 2014

Eur J Med Res

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BackgroundIn spite of the relatively high success rate of limb replantation, many patients cannot undergo replantation surgery because the preservation time of an amputated limb is only about six hours. In addition, although allotransplantation of composite tissues is being performed more commonly with increasingly greater success rates, the shortage of donors limits the number of patients that can be treated. So the purpose of this study is to examine the feasibility of cryopreservation and replantation of limbs in a rat model.MethodsTwelve five-month-old Sprague-Dawley rats were divided evenly into group A (above-knee amputation) and group B (Syme’s amputation). One hind limb was amputated from each rat. The limbs were irrigated with cryoprotectant, cooled in a controlled manner to -140°C, and placed in liquid nitrogen. Thawing and replantation were performed 14 days later.ResultsIn group A, the limbs became swollen after restoration of blood flow resulting in blood vessel compression and all replantations failed. In group B, restoration of blood flow was noted in all limbs after replantation. In one case, the rat chewed the replanted limb and replantation failed. The other five rats were followed for three months with no abnormalities noted in the replanted limbs.ConclusionsLimbs with a minimal amount of muscle tissue can be successfully cryopreserved and replanted.

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“…Slowing down metabolism and reducing high ATP consumption appears to be a key mechanism of cold storage and appears to be improved during supercooling. Preservation of organ energy status during supercooling has been confirmed in lungs (57) and hearts (58,59), where short-term supercooling preservation has been successfully performed, but transplantation has not been attempted.…”

Section: Supercoolingmentioning

confidence: 99%

Subzero organ preservation

Bruinsma

Uygun

2017

Current Opinion in Organ Transplantation

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Purpose of review Herein, we review the field of subzero organ preservation with a focus on recent developments in hepatic supercooling. Recent findings Organ preservation is making a rapid shift from the decade-old standard of storage on ice towards techniques that improve organ availability as well as preservation time. Long-term organ preservation would have tremendous benefits to the organ transplantation field, including better organ allocation, donor-recipient matching, as well as reduced preservation injury and subsequent improvement of donor organ use. The formation of ice has proven an important limiting factor and novel techniques attempt to control or prevent freezing using cryoprotective agents, and highly-controlled cooling regimens. Various techniques have been employed over the previous decades including true organ freezing, vitrification and subzero non-freezing, or supercooling. For most techniques, successful transplantation following long-term subzero preservation has remained elusive. Supercooling however recently delivered the first promising results, yielding survival after up to 4 days of supercooled preservation at −6 °C. Summary As the field of organ preservation undergoes significant development, the field of subzero preservation also receives renewed interest. While many obstacles remain to be overcome to make subzero preservation feasible, novel techniques are beginning to show their potential in achieving long-term preservation.

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Subzero 24-hr Nonfreezing Rat Heart Preservation

Abstract: The rat hearts preserved in a variable magnetic field at -3°C showed better hemodynamic and metabolic performance than those preserved using conventional storage at 4°C.

Cited by 26 publications

References 16 publications

Functional evaluation of rat hearts transplanted after preservation in a high-pressure gaseous mixture of carbon monoxide and oxygen

Functional evaluation of rat hearts transplanted after preservation in a high-pressure gaseous mixture of carbon monoxide and oxygen

Cryopreservation and replantation of amputated rat hind limbs

Subzero organ preservation

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