Success with neurotropin in treating pediatric lower extremity pain induced by spinal cord injury after epidural anesthesia

Zhu, Mengye; Zhou, Fuqing; Li, Lingchao; Yin, Qin; Qiu, Mizhen; Zhang, Yong

doi:10.2147/jpr.s135037

Cited by 8 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The drug NTP is a kind of multicomponent biological agent while NM is a chemical agent, which determines their own unique functions on microenvironment in SCI treatment. A non-protein extract isolated from inflamed rabbit skin inoculated with vaccinia virus, NTP is used to treat intractable neuropathic pain in clinical work (27,28). Analysis of its components showed that NTP was composed of more than 300 small molecular compounds and some of them have been showed neurotrophic effect, such as GABA, carnosine and adenosine.…”

Section: Discussionmentioning

confidence: 99%

Cytokine expressions of spinal cord injury treated by neurotropin and nafamostat mesylate

Sun

Duan

et al. 2021

Ann Transl Med

View full text Add to dashboard Cite

Background: Spinal cord injury (SCI) leads to severe physical disability and sensory dysfunction. Neurotropin (NTP) has been used clinically to alleviate neuropathic pain, while nafamostat mesylate (NM) used clinical on pancreatitis patients through inhibiting synthetic serine protease. Our previous studies showed that NTP and NM were able to repair SCI. However, the underlying mechanism has not been fully explored after treatment with these 2 different drugs. Methods:The drugs NTP and NM were administered on a contusion SCI Wistar rat model. Cytokine array analysis was performed to describe the changes of 67 proteins after acute SCI. Hierarchical clustering and volcano plot analysis were conducted to clarify protein change profiles. The differently expressed proteins related to biological processes were analyzed by functional protein association networks, Gene Ontology and pathway analysis. Flow cytometric analysis was detected to reflect the activation of immune system after drug intervention, while withdrawal threshold and BBB score were detected to evaluated the mechanical allodynia and functional recovery after SCI.Results: HGF, β-NGF, and activin were the 3 most upregulated proteins, while the receptor for RAGE, IL-1α, and TNF-α were the 3 most downregulated proteins after NTP treatment. Adiponectin, decorin and CTACK were the 3 most upregulated proteins, while RAGE, IL-1α, and IL-1β were the 3 most downregulated proteins in the NM group. Number of lymphocytes was decreased while BBB score was increased both in NTP and NM group. But only NTP could improve mechanical pain threshold after SCI. Conclusions:The PI3K-Akt, Jak-STAT signaling pathway and apoptosis might participate in SCI restoration by NTP, while the MAPK and NOD-like receptor signaling pathway may participated in repairing SCI with NM. We concluded that NTP regulated the microenvironment via a neuroprotective effect and inhibition of inflammation to repair SCI, while NM healed SCI through an anti-inflammatory effect. Both NTP and NM could down-regulate the activation of immune system and improve the functional recovery while only NTP could improve the pathological neuralgia after SCI. Elucidating the molecular mechanisms of these 2 clinical drugs indicates that they their expected to be effective clinical treatment for SCI.

show abstract

Section: Discussionmentioning

confidence: 99%

Cytokine expressions of spinal cord injury treated by neurotropin and nafamostat mesylate

Sun

Duan

et al. 2021

Ann Transl Med

View full text Add to dashboard Cite

show abstract

“…As NTP is prescribed to alleviate chronic pain—it activates the descending pain suppression system by enhancing noradrenergic receptors and serotonergic systems [ 6 ]—whether the drug could be used to alleviate neuropathic pain after injury warrants research. One report documented the case of a 7-year-old girl with SCI whose condition was complicated by epidural anaesthesia [ 34 ]. Moreover, NTP also reportedly features potential as an antidepressant [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Neurotropin exerts neuroprotective effects after spinal cord injury by inhibiting apoptosis and modulating cytokines

Yao

Sun

Fan

et al. 2021

Journal of Orthopaedic Translation

View full text Add to dashboard Cite

“…NTP has been widely used in Japan and China for more than 50 years as an analgesic for chronic skeletal muscle and neuropathic pain. [7][8][9] NTP contains various bioactive molecules, including nucleic acids, amino acids, and sugars. [10] Although the biologically active components responsible for its beneficial effects have not been identified, NTP exerts analgesic effects through the activation of the descending pain inhibitory system and induction of brain-derived neurotrophic factor.…”

Section: Introductionmentioning

confidence: 99%

Repurposing of the analgesic Neurotropin for MASLD/MASH treatment

Tsuchiya,

Kim,

Matsuda

et al. 2024

Hepatology Communications

View full text Add to dashboard Cite

Background: The prevalence of metabolic dysfunction–associated steatotic liver disease (MASLD) has increased in recent decades. Approximately 25% of patients with MASLD progress to metabolic dysfunction–associated steatohepatitis, which is characterized by hepatic steatosis plus hepatocyte damage, inflammation, and fibrosis. We previously reported that Neurotropin (NTP), a drug used for relieving pain in Japan and China, inhibits lipid accumulation in hepatocytes by preventing mitochondrial dysfunction. We hypothesized that inhibiting hepatic steatosis and inflammation by NTP can be an effective strategy for treating MASLD and tested this hypothesis in a MASLD mouse model. Methods: Six-week-old C57BL/6NJ male mice were fed a normal diet and normal drinking water or a high-fat diet with high fructose/glucose water for 12 weeks. During the last 6 weeks, the mice were also given high-dose NTP, low-dose NTP, or control treatment. Histologic, biochemical, and functional tests were conducted. MitoPlex, a new proteomic platform, was used to measure mitochondrial proteins, as mitochondrial dysfunction was previously reported to be associated with MASLD progression. Results: NTP inhibited the development of hepatic steatosis, injury, inflammation, and fibrosis induced by feeding a high-fat diet plus high fructose/glucose in drinking water. NTP also inhibited HSC activation. MitoPlex analysis revealed that NTP upregulated the expression of mitochondrial proteins related to oxidative phosphorylation, the tricarboxylic acid cycle, mitochondrial dynamics, and fatty acid transport. Conclusions: Our results indicate that NTP prevents the development of hepatic steatosis, injury, and inflammation by preserving mitochondrial function in the liver and inhibits liver fibrosis by suppressing HSC activation. Thus, repurposing NTP may be a beneficial option for treating MASLD/metabolic dysfunction–associated steatohepatitis.

show abstract

Success with neurotropin in treating pediatric lower extremity pain induced by spinal cord injury after epidural anesthesia

Cited by 8 publications

References 20 publications

Cytokine expressions of spinal cord injury treated by neurotropin and nafamostat mesylate

Cytokine expressions of spinal cord injury treated by neurotropin and nafamostat mesylate

Neurotropin exerts neuroprotective effects after spinal cord injury by inhibiting apoptosis and modulating cytokines

Repurposing of the analgesic Neurotropin for MASLD/MASH treatment

Contact Info

Product

Resources

About