Successful application of keratinocyte suspension using autologous fibrin spray

Johnstone, Philippa; Kwei, Johnny; Filobbos, George; Lewis, Darren; Jeffery, Steven

doi:10.1016/j.burns.2016.05.010

Cited by 21 publications

(22 citation statements)

References 13 publications

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“…One potential reason for this is that the cells themselves are delivered using a low‐viscosity spraying system and anecdotally the level of “run‐off” from the wound surface following spraying is high . Although some products now try to remedy this using fibrin glue as an adjunct, the technology has yet to find strong traction in the clinic . One of the major advantages of using a fluid gel system for the delivery of keratinocytes is that the material itself shear‐thins considerably under the rapid shear forces experienced in a spraying system.…”

Section: The Delivery Of Biological Therapeutics Using Hydrogelsmentioning

confidence: 99%

Structuring of Hydrogels across Multiple Length Scales for Biomedical Applications

et al. 2018

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The development of new materials for clinical use is limited by an onerous regulatory framework, which means that taking a completely new material into the clinic can make translation economically unfeasible. One way to get around this issue is to structure materials that are already approved by the regulator, such that they exhibit very distinct physical properties and can be used in a broader range of clinical applications. Here, the focus is on the structuring of soft materials at multiple length scales by modifying processing conditions. By applying shear to newly forming materials, it is possible to trigger molecular reorganization of polymer chains, such that they aggregate to form particles and ribbon-like structures. These structures then weakly interact at zero shear forming a solid-like material. The resulting self-healing network is of particular use for a range of different biomedical applications. How these materials are used to allow the delivery of therapeutic entities (cells and proteins) and as a support for additive layer manufacturing of larger-scale tissue constructs is discussed. This technology enables the development of a range of novel materials and structures for tissue augmentation and regeneration.

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Section: The Delivery Of Biological Therapeutics Using Hydrogelsmentioning

confidence: 99%

Structuring of Hydrogels across Multiple Length Scales for Biomedical Applications

et al. 2018

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“…Harkin et al [5], Grant et al [23], and Mittermayr et al [6] demonstrated this in pig studies using a fibrin sealant, although others failed to demonstrate a beneficial effect on graft take [7]. The latter study was, however, criticized because only small wounds were used, and the dressings used did not represent what is normally used for massive burns [16]. Although initially used as an alternative to the fibrin sealant in corneal and nerve repairs, when PRP became commercially available in the 1990s, indications rapidly expanded to include its beneficial effects on tissue healing, regeneration, and cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

“…The blood needs to be taken before the start of the surgery, as the resulting trauma will draw platelets to the operative site, diminishing the platelet concentration in the blood [15]. Activation of fibrin solidification does not require the addition of thrombin as in platelet-rich plasma but is simply accomplished by adding an alkaline buffer [16]. Where 120 ml of the patient's blood could not be provided, we have used banked whole blood to manufacture the PRF.…”

Section: Methodsmentioning

confidence: 99%

A Retrospective Study on the Use of Dermis Micrografts in Platelet-Rich Fibrin for the Resurfacing of Massive and Chronic Full-Thickness Burns

Andreone¹,

Hollander

2019

Stem Cells International

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The coverage of massive burns still represents a big challenge, even if several strategies are to date available to deal with this situation. In this study, we describe the use of a combination of platelet-rich fibrin and micrograft spray-on skin in order to increase the yield of grafted cells in patients. We treated a total of five patients, of which two were affected by massive burns and three with chronic burn wounds. Briefly, autologous micrografts were obtained by Rigenera technology using a class I medical device called Rigeneracons. The micrografts were then combined with PRF and sprayed on the wound bed by a Spraypen. Before applying PRF/micrograft spray-on skin, the wound bed was covered with an Integra® dermal template, and the wounds were dressed with a layer of antimicrobial dressing applied directly over the silicone layer. When the silicone layer of the dermal template started showing signs of separation, the wound was considered ready for grafting. In all cases, we observed a fast and complete skin graft on average after 7-10 days by PRF/micrograft spray-on skin treatment. In particular, two patients with massive burns reported rapid reepithelialization, and three patients with chronic burn wounds, two of whom had failed skin grafts before the procedure, had complete wound healing within a week. In conclusion, the results showed in this study suggest that the use of PRF/micrograft spray-on skin represents a promising approach in the management of burns or chronic burn wounds.

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“…Cultured keratinocytes can be used as cultured epithelial grafts, dermal substitutes, cultured keratinocytes can also be used in spray device systems for deep dermal burns treatment and also in aesthetical procedures and cosmetology. 9 , 10 …”

Section: Introductionmentioning

confidence: 99%

Direct differentiation of cord blood derived mesenchymal stem cells into keratinocytes without feeder layers and cAMP inducers

et al. 2020

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Objectives: The purpose of our study was isolation of umbilical cord blood derived mesenchymal stem (UCB-MSCs), their direct differentiation towards keratinocytes without using feeder layers, cAMP inducers and hormones known for morphological maintenance and proliferation of keratinocytes and characterization of UCB-MSCs through flowcytometry and keratinocytes through immunofluorescence. Methods: We have isolated and cultured UCB-MSCs (n=4) following critical parameters for successful isolation like sample processing within an hour of collection, gestational age not more than 38 weeks, no co-morbid and blood volume at least 80 ml. Cord blood mononuclear cells were isolated through ficoll based density-gradient centrifugation then cultured to isolate MSCs, defined by minimum criteria of International Society for Cellular Therapy. UCB-MSCs were then differentiated directly into keratinocytes. Differentiation was confirmed by morphology and characterized through immunofluorescence staining. UCB samples were collected from gynae/obstetric ward of OJHA campus under sterile conditions and processed at Stem cells and Regenerative medicine Lab, Dow Research Institute of Biotechnology and Biomedical Sciences, Ojha campus. The total duration of study was approximately 12 months. Results: We have successfully isolated UCB-MSCs that were plastic adherent, spindle shaped, showed trilineage mesodermal differentiation potential and were positive for CD90, CD73 and CD105 and negative for CD34 markers. UCB-MSCs were directly differentiated towards keratinocytes without using cAMP inducers, hormones or feeder layers. Differentiated keratinocytes attained typical honeycomb morphology and were stained positive on immunofluorescence for anti-pan cytokeratin antibody. Conclusion: Our study concludes possibility of direct differentiation of isolated and cultured UCB-MSCs into keratinocytes without using feeder layers and conventional keratinocyte culture media. doi: https://doi.org/10.12669/pjms.36.5.1566 How to cite this:Ghauri AK, Wahid M, Mirza T, Uddin JAA. Direct differentiation of cord blood derived mesenchymal stem cells into keratinocytes without feeder layers and cAMP inducers. Pak J Med Sci. 2020;36(5):---------. doi: https://doi.org/10.12669/pjms.36.5.1566 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Successful application of keratinocyte suspension using autologous fibrin spray

Cited by 21 publications

References 13 publications

Structuring of Hydrogels across Multiple Length Scales for Biomedical Applications

Structuring of Hydrogels across Multiple Length Scales for Biomedical Applications

A Retrospective Study on the Use of Dermis Micrografts in Platelet-Rich Fibrin for the Resurfacing of Massive and Chronic Full-Thickness Burns

Direct differentiation of cord blood derived mesenchymal stem cells into keratinocytes without feeder layers and cAMP inducers

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