Successful crosses and molecular tetrad and progeny analyses demonstrate heterothallism in Mycosphaerella graminicola

Kema, G.H.J.; Verstappen, E.C.P.; Todorova, Maria; Waalwijk, C.

doi:10.1007/s002940050129

Cited by 128 publications

(143 citation statements)

References 32 publications

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“…For this reason, the correlation between weather variables and each kind of spores was different between years. In the present work and in accordance with Shaw and Royle [31], Kema et al [32], and Hunter et al [8], the trap dates clearly showed that ascospores were formed, released, and air-dispersed all year round for the two periods.…”

Section: Discussionsupporting

confidence: 76%

Weather Conditions Associated with the Release and Dispersal of Zymoseptoria tritici Spores in the Argentine Pampas Region

Cordo

Mónaco

Altamirano

et al. 2017

International Journal of Agronomy

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The abundance of Zymoseptoria tritici ascospores and conidia in a field was examined throughout two one-year periods (1998-1999 and 1999-2000) establishing the relationship between spore release and weather variables. Radiation, temperature, intensity of rainfall, and relative humidity significantly affected the dispersal of ascospores and pycnidiospores of this pathogen. Spore traps collected both types of spores, at weekly intervals, at two different stages of the wheat crop (vegetative and wheat stubble stages) and different distances from the sources. Ascospores were the predominant sources of inoculum in the field. The numbers of ascospores and pycnidiospores declined with the increase of distance from the sources. The release of pycnidiospores was associated with the increase in rainfall intensity 7 days before the released event and the increase in radiation 60 days before the same event.Relative humidity 3 and 15 days before the release event was positively correlated with ascospores release and negatively correlated with radiation and temperature in all the sampling interval. Also for the first time, a positive correlation between radiation and pycnidiospores dispersal is reported. Understanding the relationship between environment conditions and spores dispersal event could improve the control strategies of the disease.

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Section: Discussionsupporting

confidence: 76%

Weather Conditions Associated with the Release and Dispersal of Zymoseptoria tritici Spores in the Argentine Pampas Region

Cordo

Mónaco

Altamirano

et al. 2017

International Journal of Agronomy

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“…Briefly, four Swiss wildtype strains were used to create two mapping populations, following an established protocol (Kema et al, 1996). The mapping populations were composed of 263 (ST99CH3D1 (3D1: SRS383146) × ST99CH3D7 (3D7: SRS383147)) and 261 (ST99CH1A5 (1A5: SRS383142) × ST99CH1E4 (1E4: SRS383143)) retained progeny.…”

Section: Methodsmentioning

confidence: 99%

QTL mapping of temperature sensitivity reveals candidate genes for thermal adaptation and growth morphology in the plant pathogenic fungus Zymoseptoria tritici

et al. 2016

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Different thermal environments impose strong, differential selection on populations, leading to local adaptation, but the genetic basis of thermal adaptation is poorly understood. We used quantitative trait locus (QTL) mapping in the fungal wheat pathogen Zymoseptoria tritici to study the genetic architecture of thermal adaptation and identify candidate genes. Four wild-type strains originating from the same thermal environment were crossed to generate two mapping populations with 263 (cross 1) and 261 (cross 2) progeny. Restriction site-associated DNA sequencing was used to genotype 9745 (cross 1) and 7333 (cross 2) singlenucleotide polymorphism markers segregating within the mapping population. Temperature sensitivity was assessed using digital image analysis of colonies growing at two different temperatures. We identified four QTLs for temperature sensitivity, with unique QTLs found in each cross. One QTL had a logarithm of odds score 411 and contained only six candidate genes, including PBS2, encoding a mitogen-activated protein kinase kinase associated with low temperature tolerance in Saccharomyces cerevisiae. This and other QTLs showed evidence for pleiotropy among growth rate, melanization and growth morphology, suggesting that many traits can be correlated with thermal adaptation in fungi. Higher temperatures were highly correlated with a shift to filamentous growth among the progeny in both crosses. We show that thermal adaptation has a complex genetic architecture, with natural populations of Z. tritici harboring significant genetic variation for this trait. We conclude that Z. tritici populations have the potential to adapt rapidly to climate change and expand into new climatic zones.

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“…The higher susceptibility of wheat genotypes could be mainly due to prevailing climatic conditions suitable for its development (frequent rains and moderate temperature) (Gilchrist and Dubin, 2002;Abebe et al, 2015) and have a wider virulence spectrum of the pathogen population. The population of Septoria tritici blotch is highly diverse genetically and the fungus may reproduce sexually several times during the wheat-growing season (Kema et al, 1996;McDonald et al, 1999;Abebe et al, 2015). According to Razavi (2003), a high degree of variability at the lesion sampling level and a low degree of variability at the leaf and location sampling levels suggest that the primary source of inoculum was most likely air-borne ascospores, which would have been dispersed evenly across the field.…”

Section: Prevalence and Severity Of Septoria Leaf Blotchmentioning

confidence: 99%

Evaluation of disease intensity and molecular identification of Zymoseptoria tritici causing Septoria leaf blotch on wheat in the Eastern Mediterranean Region of Turkey

Ünal¹,

Kayim²,

Ay³

et al. 2017

Turk J Agric For

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Wheat is one of the most important crops in Turkey. Production of this crop is constrained by the disease Septoria leaf blotch, which is a major bottleneck in wheat production in the Eastern Mediterranean Region of Turkey. In this study, 260 different wheat fields were surveyed for the estimation of prevalence and severity of Septoria leaf blotch. Out of 100% of the total wheat crop fields, 58.91% were infected with this disease. The mean value of Septoria leaf blotch virulence was 11.62%. Out of 142 diseased wheat crop samples, 74 isolates were used for the pathogenicity test. Forty-seven isolates from Adana, 3 isolates from Mersin, 3 isolates from Osmaniye, 14 isolates from Hatay, and 7 isolates from Kahramanmaraş provinces were cultured on PDA plates. The cause of disease agent was identified based on microscopic observation of morphological structure and molecular analysis methods. Consequently, total 15 virulent isolates, 3 isolates from each province were sequenced for 5′ and 3′ ends of ITS of rDNA and nBLAST of the ITS sequences revealed that Zymoseptoria tritici was the agent of Septoria leaf blotch. The highest disease prevalence (87.99%) was recorded at Adana and followed by Osmaniye with 87.44%, Kahramanmaraş (43.59%), Mersin (42.68%), and Hatay (26.03%).

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Successful crosses and molecular tetrad and progeny analyses demonstrate heterothallism in Mycosphaerella graminicola

Cited by 128 publications

References 32 publications

Weather Conditions Associated with the Release and Dispersal of Zymoseptoria tritici Spores in the Argentine Pampas Region

Weather Conditions Associated with the Release and Dispersal of Zymoseptoria tritici Spores in the Argentine Pampas Region

QTL mapping of temperature sensitivity reveals candidate genes for thermal adaptation and growth morphology in the plant pathogenic fungus Zymoseptoria tritici

Evaluation of disease intensity and molecular identification of Zymoseptoria tritici causing Septoria leaf blotch on wheat in the Eastern Mediterranean Region of Turkey

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