Successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders

Alçay, Selim; Çakmak, Selvinar Seven; Çakmak, İbrahim; Mülkpinar, Emine; Gökçe, Elif; Üstüner, Burcu; Şen, Hasan; Nur, Zekariya

doi:10.1016/j.cryobiol.2019.03.005

Cited by 27 publications

(29 citation statements)

References 22 publications

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“…In this study, Acrosome integrity was the highest in the first concentration as compared to the control and other concentrations . Our results agreed with Alcay et al (2019) [30] . In this study, the superior in vivo sperm fertility expressed as conception rate (CR) of the post-thawed frozen semen used in artificial insemination were given with the first , second and the third concentrations of royal jelly and decreased at the last two concentrations .…”

Section: Discussionsupporting

confidence: 94%

“…In the current study, the plasma membrane integrity parameters were higher than the control at post thaw. These results are in agreement with Alcay et al [15,30].…”

Section: Discussionsupporting

confidence: 93%

“…Royal jelly ameliorated the fatty acid peroxidation as indicated by the lowered levels of MDA (9,25). Royal jelly has antioxidant effect on goat and fish semen freezing [20,30]. Royal jelly is one of the important antioxidant additives, since it has an excellent protective functions exerted from its amino acid content [20,21 ].…”

Section: Discussionmentioning

confidence: 99%

“…[2019] [30] who found that RJ supplementation caused an obvious increase on motility .However, higher concentrations of RJ revealed a slow decline in sperm motility.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Royal Jelly Tris-enriched Extender on Buffalo Semen Cryopreservation

El-Sheshtawy¹

2020

NIDOC-ASRT

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Royal Jelly (bee's milk) is produced by the hypo-pharyngeal and mandibular glands of the worker honey bees and this is considered the primary food of the honey bee queen and honey bee larvae during the first three days of its life [5]. Royal jelly is composed of proteins, lipids, sugars, vitamins and essential amino acids [6,7]. Royal jelly has a beneficial biological activity in different cells and tissues of laboratory animals [8]. Royal jelly has antibacterial effect [9]. The objective of this study was to elucidate the protective effect of royal jelly addition in semen extender on semen quality post-cooling and postfreezing and on conception rate.

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Section: Discussionsupporting

confidence: 94%

“…In the current study, the plasma membrane integrity parameters were higher than the control at post thaw. These results are in agreement with Alcay et al [15,30].…”

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

“…[2019] [30] who found that RJ supplementation caused an obvious increase on motility .However, higher concentrations of RJ revealed a slow decline in sperm motility.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Royal Jelly Tris-enriched Extender on Buffalo Semen Cryopreservation

El-Sheshtawy¹

2020

NIDOC-ASRT

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“…In the more recent studies, sperm motility has been more frequently expressed as the percentage of motile cells, with some occasional indicators of the type of movement [20,25,74,89,94,95]. Spermatozoa may be classified as motile sperm if they present any type of active movement, freely motile if the sperm head shows displacement, and circular sperm if the sperm head and tail overlap [89].…”

Section: Sperm Motilitymentioning

confidence: 99%

Sperm Quality Assessment in Honey Bee Drones

Yániz

Silvestre

Santolaria

2020

Biology

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The quality of honey bee drone semen is relevant in different contexts, ranging from colony productivity to pathology, toxicology and biodiversity preservation. Despite its importance, considerably less knowledge is available on this subject for the honey bee when compared to other domestic animal species. A proper assessment of sperm quality requires a multiple testing approach which discriminates between the different aspects of sperm integrity and functionality. Most studies on drone semen quality have only assessed a few parameters, such as sperm volume, sperm concentration and/or sperm plasma membrane integrity. Although more recent studies have focused on a broader variety of aspects of semen quality, some techniques currently used in vertebrates, such as computer-assisted sperm analysis (CASA) or multiparametric sperm quality testing, still remain to be developed in the honey bee. This may be attributed to the particular sperm morphology and physiology in this species, requiring the development of technologies specifically adapted to it. This article reviews the present knowledge of sperm quality in honey bee drones, highlighting its peculiarities and proposing future lines of research.

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Methionine, cysteine, and butylated hydroxytoluene enhance cryosurvival of ram semen on post-thaw and post-incubation time points

Toker

Doğan

2023

Trop Anim Health Prod

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Despite there have been many experiments conducted about antioxidants, the best sole or combination use of antioxidants to include as a standard ingredient to freezing extenders yet to be found. This study conducted to investigate the different doses of methionine (2.5 and 5mM), cysteine (1 and 2mM) and butylated hydroxytoluene (BHT) (1 and 2mM) for ram semen cryopreservation on post-thaw and postincubation (6 h) time points over spermatological parameters. Semen samples collected from Kivircik rams via electro-ejaculator in breeding season. After essential spermatological evaluations, appropriate samples were pooled then split into 7 equal aliquots to create study groups (antioxidant free control, 2.5mM methionine, 5mM methionine, 1mM cysteine, 2mM cysteine, 1mM BHT and 2mM BHT). Semen samples put into French straws (0.25mL) and freezing procedure (two-step) conducted via a programmable gamete freezer. At both time points; motility, HOST, PSA-FITC and TUNEL assays were conducted to discover the impacts of cryopreservation and incubation process over sperm cells.Antioxidant supplemented groups yielded better results comparing to control groups in terms of various spermatological parameters not only at post-thaw time point but after incubation for 6 h of time. The study demonstrated that supplementing sperm freezing extenders with abovesaid antioxidants may create new approaches to cryopreservation procedures and through increasing success rate of freezing, fertility results may increase to better results in near future.

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Successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders

Cited by 27 publications

References 22 publications

Effect of Royal Jelly Tris-enriched Extender on Buffalo Semen Cryopreservation

Effect of Royal Jelly Tris-enriched Extender on Buffalo Semen Cryopreservation

Sperm Quality Assessment in Honey Bee Drones

Methionine, cysteine, and butylated hydroxytoluene enhance cryosurvival of ram semen on post-thaw and post-incubation time points

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