Successful Gene Transfer Into Murine Pancreatic Islets Using Polyamine Transfection Reagents

Kenmochi, Takashi; Asano, T.; Nakagori, Toshio; Kikuchi, Kentaro; Nakajima, Kiyokazu; Tetsu, Osamu; Jingu, Kazuhiko; Iwashita, C.; Kainuma, Osamu; Tokoro, Yoshiharu; Sugamoto, Yuji; Sakamoto, Kana; Hatakeyama, Emiko; Yamada, Katsumi; Isono, Kaichi

doi:10.1016/s0041-1345(97)01360-2

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A Nonviral Gene Delivery1

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2006

2010

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“…Although we and others have shown the feasibility of transfection of islets with nonviral vectors (Kenmochi et al, 1998;Lakey et al, 2001b;Mahato et al, 2003;Narang et al, 2004Narang et al, , 2005, transfection efficiency remains low. Further efforts are needed to investigate the role of plasmid vector and gene carrier design considerations to improve transfection efficiency.…”

Section: A Nonviral Gene Deliverymentioning

confidence: 92%