Successful immunotherapy in a murine metastasizing fibrosarcoma model

Cortés, R. Peña; Correa, Luis Alfonso; Behbehani, A; Sonis, Stephen T.; Wilson, Richard E.

doi:10.1002/jso.2930250415

Cited by 2 publications

(9 citation statements)

References 19 publications

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“…We have shown previously that immunotherapy with live tumor cells is effective in a model of minimal residual tumor bearing syngeneic mice [1,2,5,14]. The results indicated that therapy with live tumor cells improved the survival rate significantly, and was even more effective when tumor cells from metastatic sites (lung or liver) were combined with primary tumor cells.…”

Section: Introductionmentioning

confidence: 64%

“…The tumor grows progressively at the injected site and metastasizes spontaneously to liver or lung or both. Mice succumb within 30 days if untreated [2].…”

Section: Methodsmentioning

confidence: 99%

“…The method of tumor cell suspension preparation was the same as the previously used [2]. Briefly, tumors were excised, minced, and treated with trypsin.…”

Section: Methodsmentioning

confidence: 99%

“…patic metastases. The latter were determined at autopsy using the same technique as previously described [2].…”

Section: Methodsmentioning

confidence: 99%

“…The present study was designed to answer the following questions: (1) Is immunotherapy in the form of irradiated tumor cells more or less effective than live cell immunotherapy? (2) What is the optimum cell dose? * Research fellow from Zhejiang Medical University, Hongzhou, China ** Supported by the Brigham Surgical Group, Inc. Offprint requests to : Stephen Sonis …”

Section: Introductionmentioning

confidence: 99%

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Increased efficiency of immunotherapy using irradiated tumor cells

Fitzgerald

Sonis

et al. 1987

Cancer Immunol Immunother

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The efficacy of irradiated tumor cells combined with chemotherapy or non-specific immunostimulation with complete Freund's adjuvant was tested in a model of minimal residual tumor-bearing syngeneic mice. Male C57BL/6J mice were innoculated in the right rear leg with live tumor cells from a methylcholanthrene induced fibrosarcoma. The tumor was resected when it reached 0.7 cm in diameter and animals were treated with doses of irradiated tumor cells (XTC) from the primary tumor ranging in number from 1 X 10(3) to 9 X 10(3). Best survival was noted using 5 X 10(3) XTC combined with irradiated tumor cells of liver or pulmonary metastases origin, complete Freund's adjuvant or cytoxan. The combination of irradiated tumor cells of metastatic origin did not enhance the therapeutic effect of XTC alone. Freund's adjuvant was not of benefit in enhancing the efficacy of XTC. However, improved survival was noted when chemotherapy in the form of cytoxan was used to supplement XTC. Our data suggests that XTC is more efficacious as a mode of immunotherapy than are live tumor cells. The dose of XTC used is critical in determining its effect. Chemotherapy appears to enhance the benefit of XTC.

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Section: Introductionmentioning

confidence: 64%

“…The tumor grows progressively at the injected site and metastasizes spontaneously to liver or lung or both. Mice succumb within 30 days if untreated [2].…”

Section: Methodsmentioning

confidence: 99%

“…The method of tumor cell suspension preparation was the same as the previously used [2]. Briefly, tumors were excised, minced, and treated with trypsin.…”

Section: Methodsmentioning

confidence: 99%

“…patic metastases. The latter were determined at autopsy using the same technique as previously described [2].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Increased efficiency of immunotherapy using irradiated tumor cells

Fitzgerald

Sonis

et al. 1987

Cancer Immunol Immunother

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In vivoandin vitroeffects of β‐carotene and algae extracts in murine tumor models

Combs

Sonis

Fitzgerald

et al. 1989

Nutrition and Cancer

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Phycotene, an algae extract with known antineoplastic activity, was demonstrated to prolong, but not sustain, an increased survival rate in a murine fibrosarcoma model when it was combined with immunotherapy. It was further shown that splenocytes from phycotene and beta-carotene-treated survivors could not confer protection to a fresh tumor cell challenge in virgin mice after adoptive transfer. In a series of cytotoxicity assays, phycotene combined with immunization was demonstrated to enhance cell-mediated and complement-dependent cytotoxicity in the first 14-21 days. However, after 21 days, the phycotene and immunization groups exhibited a decreased ability to mediate immune cytotoxicity compared with immunization-only controls. This may serve to explain the in vivo findings that while survival was increased early on in active immunization and phycotene-treated mice, it eventually dropped to the level of the active immunization controls.

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Successful immunotherapy in a murine metastasizing fibrosarcoma model

Cited by 2 publications

References 19 publications

Increased efficiency of immunotherapy using irradiated tumor cells

Increased efficiency of immunotherapy using irradiated tumor cells

In vivoandin vitroeffects of β‐carotene and algae extracts in murine tumor models

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