Successful implantation of in vitro-matured, electro-activated oocytes in the pig

Kure-bayashi, S.; Miyake, Masakazu; Okada, Konosuke; Kato, Seishiro

doi:10.1016/s0093-691x(00)00256-9

Cited by 96 publications

(71 citation statements)

References 24 publications

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“…When mammalian oocytes are activated (emulating the fertilization process) and transferred to a surrogate mother, they are capable of surviving to day 10 of development in the mouse, day 21 for sheep, day 29 in pigs, and day 11.5 in rabbit (9)(10)(11)(12).…”

mentioning

confidence: 99%

Nonhuman primate parthenogenetic stem cells

Vrana

Hipp

Goss

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

176

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Parthenogenesis is the biological phenomenon by which embryonic development is initiated without male contribution. Whereas parthenogenesis is a common mode of reproduction in lower organisms, the mammalian parthenote fails to produce a successful pregnancy. We herein describe in vitro parthenogenetic development of monkey (Macaca fascicularis) eggs to the blastocyst stage, and their use to create a pluripotent line of stem cells. These monkey stem cells (Cyno-1 cells) are positive for telomerase activity and are immunoreactive for alkaline phosphatase, octamer-binding transcription factor 4 (Oct-4), stage-specific embryonic antigen 4 (SSEA-4), tumor rejection antigen 1-60 (TRA 1-60), and tumor rejection antigen 1-81 (TRA 1-81) (traditional markers of human embryonic stem cells). They have a normal chromosome karyotype (40 + 2) and can be maintained in vitro in an undifferentiated state for extended periods of time. Cyno-1 cells can be differentiated in vitro into dopaminergic and serotonergic neurons, contractile cardiomyocyte-like cells, smooth muscle, ciliated epithelia, and adipocytes. When Cyno-1 cells were injected into severe combined immunodeficient mice, teratomas with derivatives from all three embryonic germ layers were obtained. When grown on fibronectin/laminin-coated plates and in neural progenitor medium, Cyno-1 cells assume a neural precursor phenotype (immunoreactive for nestin). However, these cells remain proliferative and express no functional ion channels. When transferred to differentiation conditions, the nestin-positive precursors assume neuronal and epithelial morphologies. Over time, these cells acquire electrophysiological characteristics of functional neurons (appearance of tetrodotoxin-sensitive, voltage-dependent sodium channels). These results suggest that stem cells derived from the parthenogenetically activated nonhuman primate egg provide a potential source for autologous cell therapy in the female and bypass the need for creating a competent embryo

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mentioning

confidence: 99%

Nonhuman primate parthenogenetic stem cells

Vrana

Hipp

Goss

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

176

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show abstract

“…In mammals, both maternal and paternal genomes are usually required for full development. Parthenogenotes can develop to the mid-gestation stage but not to term in most cases [20][21][22][23][24][25][26]. Live mice have been reported to be produced from pathenogenotes, however, through modifications in genomic imprinting [27].…”

Section: Introductionmentioning

confidence: 99%

Nuclear reprogramming: the strategy used in normal development is also used in somatic cell nuclear transfer and parthenogenesis

et al. 2007

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Somatic cell nuclear transfer (SCNT) and parthenogenesis are alternative forms of reproduction and development, building new life cycles on differentiated somatic cell nuclei and duplicated maternal chromatin, respectively. In the preceding paper (Sun F, et al., Cell Res 2007; 17:117-134.), we showed that an "erase-and-rebuild" strategy is used in normal development to transform the maternal gene expression profile to a zygotic one. Here, we investigate if the same strategy also applies to SCNT and parthenogenesis. The relationship between chromatin and chromatin factors (CFs) during SCNT and parthenogenesis was examined using immunochemical and GFP-fusion protein assays. Results from these studies indicated that soon after nuclear transfer, a majority of CFs dissociated from somatic nuclei and were redistributed to the cytoplasm of the egg. The erasure process in oogenesis is recaptured during the initial phase in SCNT. Most CFs entered pseudo-pronuclei shortly after their formation. In parthenogenesis, all parthenogenotes underwent normal oogenesis, and thus had removed most CFs from chromosomes before the initiation of development. The CFs were subsequently re-associated with female pronuclei in time and sequence similar to that in fertilized embryos. Based on these data, we conclude that the "erase-and-rebuild" process observed in normal development also occurs in SCNT and in parthenogenesis, albeit in altered fashions. The process is responsible for transcription reprogramming in these procedures. The "erase" process in SCNT is compressed and the efficiency is compromised, which likely contribute to the developmental defects often observed in nuclear transfer (nt) embryos. Furthermore, results from this study indicated that the cytoplasm of an egg contains most, if not all, essential components for assembling the zygotic program and can assemble them onto appropriate diploid chromatin of distinct origins.

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“…Co-transfer of parthenogenetic oocytes may be an aid to successful implantation (Kawarasaki et al 2009). Porcine parthenogenotes can be implanted and can remain viable during the early stage of pregnancy, but they cannot develop beyond the 29th day after transfer (Kure-bayashi et al 2000). In the present study, because we obtained piglets only in the co-transferred group (Supplementary Table 1), co-transfer of parthenogenotes may facilitate the establishment of pregnancy and term.…”

Section: Resultsmentioning

confidence: 81%

Production of viable piglets for the first time using sperm derived from ectopic testicular xenografts

Nakai¹,

Kaneko²,

Somfai³

et al. 2010

Reproduction

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Xenografting of testicular tissue into immunodeficient mice is known to be a valuable tool for facilitating the development of immature germ cells present in mammalian gonads. Spermatogenesis in xenografts and/or in vitro embryonic development to the blastocyst stage after ICSI of xenogeneic sperm has already been reported in large animals, including pigs; however, development of the embryos to term has not yet been confirmed. Therefore, in pigs, we evaluated the in vivo developmental ability of oocytes injected after ICSI of xenogeneic sperm. Testicular tissues prepared from neonatal piglets, which contain seminiferous cords consisting of only gonocytes/spermatogonia, were transplanted under the back skin of castrated nude mice. Between 133 and 280 days after xenografting, morphologically normal sperm were recovered, and a single spermatozoon was then injected into an in vitro matured porcine oocyte. After ICSI, the oocytes were electrostimulated and transferred into estrus-synchronized recipients. Two out of 23 recipient gilts gave birth to six piglets. Here, we describe for the first time that oocytes fertilized with a sperm from ectopic xenografts have the ability to develop to viable offspring in large mammals.

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Successful implantation of in vitro-matured, electro-activated oocytes in the pig

Cited by 96 publications

References 24 publications

Nonhuman primate parthenogenetic stem cells

Nonhuman primate parthenogenetic stem cells

Nuclear reprogramming: the strategy used in normal development is also used in somatic cell nuclear transfer and parthenogenesis

Production of viable piglets for the first time using sperm derived from ectopic testicular xenografts

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