Successful intercalation of DNA into CTAB-modified clay minerals for gene protection

Hou, Yakun; Wu, Pingxiao; Huang, Zhujian; Ruan, Bo; Liu, Paiyu; Zhu, Ning

doi:10.1007/s10853-014-8435-5

Cited by 14 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, the previous transport, magnetization and specific heat measurements showed that there is no clear indication of strong electron correlations in the electronic properties of Cu pyrites. [11] It is chalcogen 𝑝 character rather than 𝑑 characteristic of Cu that dominates at the Fermi level, [11,12] which is in remarkable contrast to other 3𝑑 transition metal pyrites.…”

mentioning

confidence: 99%

Observation of Tunneling Gap in Epitaxial Ultrathin Films of Pyrite-Type Copper Disulfide

Liu

Yang

Song

et al. 2018

Chinese Phys. Lett.

View full text Add to dashboard Cite

We report scanning tunneling microscopy investigation on epitaxial ultrathin films of pyrite-type copper disulfide.Layer by layer growth of CuS2 films with a preferential orientation of (111) on SrTiO3 (001) and Bi2Sr2CaCu2O8+δ substrates is achieved by molecular beam epitaxy growth. For ultrathin films on both kinds of substrates, we observed symmetric tunneling gap around Fermi level that persists up to ~ 15 K. The tunneling gap degrades with either increasing temperature or increasing thickness, suggesting new matter states at the extreme two dimensional limit.

show abstract

mentioning

confidence: 99%

Observation of Tunneling Gap in Epitaxial Ultrathin Films of Pyrite-Type Copper Disulfide

Liu

Yang

Song

et al. 2018

Chinese Phys. Lett.

View full text Add to dashboard Cite

show abstract

“…Investigations of 16 on the effect of pH on the adsorption of DNA to clay minerals have shown that adsorption capacity decreased at higher pH [24,36]. Recently, Hou et al [47] demonstrated that clay minerals modified with CTAB have a greater capacity for DNA adsorption and intercalation of DNA into the clay galleries. This mechanism may contribute to the overall low performance of CTAB-based protocols on clay soils.…”

Section: Discussionmentioning

confidence: 99%

Improved Protocol for DNA Extraction from Subsoils Using Phosphate Lysis Buffer

et al. 2020

View full text Add to dashboard Cite

As our understanding of soil biology deepens, there is a growing demand for investigations addressing microbial processes in the earth beneath the topsoil layer, called subsoil. High clay content in subsoils often hinders the recovery of sufficient quantities of DNA as clay particles bind nucleic acids. Here, an efficient and reproducible DNA extraction method for 200 mg dried soil based on sodium dodecyl sulfate (SDS) lysis in the presence of phosphate buffer has been developed. The extraction protocol was optimized by quantifying bacterial 16S and fungal 18S rRNA genes amplified from extracts obtained by different combinations of lysis methods and phosphate buffer washes. The combination of one minute of bead beating, followed by ten min incubation at 65°C in the presence of 1 M phosphate buffer with 0.5% SDS, was found to produce the best results. The optimized protocol was compared with a commonly used cetyltrimethylammonium bromide (CTAB) method, using Phaeozem soil collected from 60 cm depth at a conventional agricultural field and validated on five subsoils. The reproducibility and robustness of the protocol was corroborated by an interlaboratory comparison. The DNA extraction protocol offers a reproducible and cost-effective tool for DNA-based studies of subsoil biology.

show abstract

“…discussed the role of clay minerals and layered double hydroxides in various biological applications (Choy et al ., 2007). Intercalation of DNA into CTAB-modified clay minerals for gene protection concluded that CTAB-clay/DNA hybrids are potential materials for the storage of genetic information (Hou et al ., 2014). Brack (2013) and Ponnamperuma et al .…”

Section: Introductionmentioning

confidence: 99%

Prebiotic studies on the interaction of zirconia nanoparticles and ribose nucleotides and their role in chemical evolution

Arora¹,

Kumar

2021

International Journal of Astrobiology

View full text Add to dashboard Cite

Studies on the interaction of biomolecules with inorganic compounds, mainly mineral surfaces, are of great concern in identifying their role in chemical evolution and origins of life. Metal oxides are the major constituents of earth and earth-like planets. Hence, studies on the interaction of biomolecules with these minerals are the point of concern for the study of the emergence of life on different planets. Zirconium oxide is one of the metal oxides present in earth's crust as it is a part of several types of rocks found in sandy areas such as beaches and riverbeds, e.g. pebbles of baddeleyite. Different metal oxides have been studied for their role in chemical evolution but no studies have been reported about the role of zirconium oxide in chemical evolution and origins of life. Therefore, studies were carried out on the interaction of ribonucleic acid constituents, 5′-CMP (cytidine monophosphate), 5′-UMP (uridine monophosphate), 5′-GMP (guanosine monophosphate) and 5′-AMP (adenosine monophosphate), with zirconium oxide. Synthesized zirconium oxide particles were characterized by using vibrating sample magnetometer, X-Ray Diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy. Zirconia particles were in the nanometre range, from 14 to 27 nm. The interaction of zirconium oxide with ribonucleic acid constituents was performed in the concentration range of 5 × 10−5–300 × 10−5 M. Interaction studies were carried out in three mediums; acidic (pH 4.0), neutral (pH 7.0) and basic (pH 9.0). At neutral pH, maximum interaction was observed. The interaction of zirconium oxide with 5′-UMP was 49.45% and with 5′-CMP 67.98%, while with others it was in between. Interaction studies were Langmurian in nature. Xm and KL values were calculated. Infrared spectral studies of ribonucleotides, metal oxide and ribonucleotide–metal oxide adducts were carried out to find out the interactive sites. It was observed that the nitrogen base and phosphate moiety of ribonucleotides interact with the positive charge surface of metal oxide. SEM was also carried out to study the adsorption. The results of the present study favour the important role of zirconium oxide in concentrating the organic molecules from their dilute aqueous solutions in primeval seas.

show abstract

Successful intercalation of DNA into CTAB-modified clay minerals for gene protection

Cited by 14 publications

References 37 publications

Observation of Tunneling Gap in Epitaxial Ultrathin Films of Pyrite-Type Copper Disulfide

Observation of Tunneling Gap in Epitaxial Ultrathin Films of Pyrite-Type Copper Disulfide

Improved Protocol for DNA Extraction from Subsoils Using Phosphate Lysis Buffer

Prebiotic studies on the interaction of zirconia nanoparticles and ribose nucleotides and their role in chemical evolution

Contact Info

Product

Resources

About