Successful intracytoplasmic sperm injection with spermatozoa from a patient with dysplasia of the fibrous sheath and chronic respiratory disease

Olmedo, S. Brugo; Nodar, F.; Chillik, Claudio F.; Chemes, H

doi:10.1093/humrep/12.7.1497

Cited by 41 publications

(18 citation statements)

References 9 publications

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“…Olmedo and colleagues used ejaculated spermatozoa from a male with a combination of dysplasia of the fibrous sheath and dynein arm deficiency. Three embryos were transferred at the twocell stage which resulted in the birth of a healthy baby girl [10]. Von Zumbusch et al treated two couples with KS.…”

Section: Discussionmentioning

confidence: 99%

“…Successful treatment of PCD with the birth of healthy babies following embryo transfer on day three or earlier has been reported using sub-zonal insemination (SUZI) [4][5][6] and intracytoplasmic sperm injection (ICSI) [7][8][9][10][11][12][13]. The hypo-osmotic swelling (HOS) test [14] was used in conjunction with ICSI of immotile spermatozoa with success in several cases as well [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

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Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Kordus

Price

Davis

et al. 2008

J Assist Reprod Genet

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Purpose To describe the ultrastructure of spermatozoa from a patient with complete asthenozoospermia that resulted in live births following blastocyst culture. Materials and methods Analyses of spermatozoa from a 36 year old patient were performed using light and electron microscopy. The hypo-osmotic swelling test was used to select spermatozoa for intracytoplasmic sperm injection. Embryos were cultured to the blastocyst stage. Results 100% of the spermatozoa had dynein arm deficiency with secondary defects varying from 3-17%. Six oocytes were injected; five fertilized normally and one was digynic. All five zygotes formed good quality blastocysts. Three blastocysts were cryopreserved and two blastocysts were transferred. Twin females were born at 37 weeks. Conclusions The hypo-osmotic swelling test can be used to select viable immotile ejaculated spermatozoa from a patient with dynein arm deficiency and can produce excellent fertilization rates and blastocyst development resulting in live births.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Kordus

Price

Davis

et al. 2008

J Assist Reprod Genet

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“…However, in the literature good fertilization rate, pregnancies, and live births were reported by few reports for DFS disorder. As reviewed by Garza and Patrizio [41] a successful pregnancy after ICSI with the birth of a healthy baby was reported by Olmedo et al, [42] for one DFS patient. Chemes and Rawe [4] described ICSI results for 12 patients presented with DFS.…”

Section: Discussionmentioning

confidence: 89%

Meiotic segregation and sperm DNA fragmentation in Tunisian men with dysplasia of the fibrous sheath (DFS) associated with head abnormalities

Ghédir

Mehri

Mogharrabi

et al. 2014

J Assist Reprod Genet

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Purpose Dysplasia of the Fibrous Sheath (DFS) is a primitive flagellar pathology for which a broad spectrum of ultrastructural flagellar abnormalities has been described responsible for a severe to total asthenozoospermia. To this phenotype other morphological abnormalities including cephalic and abnormalities in nuclear structure can be associated that could compromise embryonic development in case of use of Assisted Reproductive Technology (ART). The aim of this study was to evaluate the level of DNA fragmentation and aneuploidy rate in ejaculated spermatozoa of Tunisian men presented with DFS sperm defect associated to high percentage of head abnormalities and to compare the results with those from fertile men. Methods Sperm DNA fragmentation was evaluated by the terminal desoxynucleotidyl transferase mediated deoxyuridine triphosphate biotin nick-end labelling (TUNEL) assay. The study of meiotic segregation was performed by Fluorescence in situ hybridization (FISH) for chromosomes X, Y and 18. Results The mean DNA fragmentation index was significantly higher in patients compared to the control group. FISH revealed a significantly higher incidence of sperm aneuploidies compared with controls. All patients showed elevated frequencies of sex chromosomes disomy, disomy 18 and diploidy. Conclusions In some cases of syndromic teratozoospermia due to sperm tail structural abnormalities, such as DFS, other morphological cephalic abnormalities may be associated. In these cases we have demonstrated impaired sperm nuclear quality which will affect the results in ICSI. Hence the interest of a thorough study of the sperm nucleus in these forms of infertility in order to predict the chances of success in ART.

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“…1 There have, however, been some successful intracytoplasmic sperm injection (ICSI) fertilisations that have resulted in pregnancy and live births. 49,55,56 In view of the likely genetic origin of DFS, an accurate diagnosis is considered to be important, so that counselling can be offered to the patients. 51 The light microscopy appearance of short thickened sperm tails can be indicative of other conditions such as necrospermia.…”

Section: Dysplasia Of the Fibrous Sheathmentioning

confidence: 99%

“…The ICSI technique has now been successfully utilised to produce fertilisation using sperm with severe structural tail defects. These include dysplasia of the fibrous sheath 49,55,56 and ciliary dyskinesia. 71,72 With the emergence of such methods to overcome the natural barriers to fertilisation, the investigation of male infertility may nowadays be considered less important.…”

Section: Concluding Commentsmentioning

confidence: 99%

Electron microscopy in the investigation of asthenozoospermia

Mobberley

2010

British Journal of Biomedical Science

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Asthenozoospermia, defined as low sperm motility, is a significant cause of subfertility in men. Its origins are diverse and in some instances cannot be ascertained. However, severely reduced motility can often be associated with abnormalities in the structure of the sperm tails, which can only be detected by transmission electron microscopy (TEM). In this respect, TEM is an important adjunct to the traditional methods of semen analysis. This review examines the development of the current state of knowledge of sperm tail abnormalities. These may be genetic in origin, or they may be acquired as a result of extrinsic factors. At present, consistent molecular markers are not available to characterise many of the genetic defects. However, TEM can distinguish specific defects of genetic origin and the non-specific structural anomalies that are typical of an acquired condition. It can also differentiate sperm structural anomalies from necrospermia, or sperm death, which is another significant cause of asthenozoospermia. In this modern era of assisted reproduction, it is possible in some instances to circumvent the problems of sperm immotility and to achieve fertilisation and pregnancy using intracytoplasmic sperm injection (ICSI). However, because of the possible genetic origin of asthenozoospermia, many scientists working in the field of infertility believe that it is of the utmost importance to investigate the causes of asthenozoospermia. This review considers the continuing relevance of TEM to the evaluation of sperm tail abnormalities in the context of current reproductive techniques.

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Successful intracytoplasmic sperm injection with spermatozoa from a patient with dysplasia of the fibrous sheath and chronic respiratory disease

Cited by 41 publications

References 9 publications

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Meiotic segregation and sperm DNA fragmentation in Tunisian men with dysplasia of the fibrous sheath (DFS) associated with head abnormalities

Electron microscopy in the investigation of asthenozoospermia

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