Successful Intrasplenic Autotransplantation of Pancreatic Tissue in Totally Pancreatectomised Dogs

Mirkovitch,; Campiche, M

doi:10.1097/00007890-197603000-00014

Cited by 92 publications

(38 citation statements)

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“…10 Mincing of the pancreas causes a 50% loss of tissue insulin. 11 Incubation of the chopped pancreas in collagenase destroys more than 90% of the islet as well as the acinar tissue. Therefore, with this technique less than a 10% yield of the total islet cell mass of the original gland can be expected.…”

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confidence: 99%

Preparation of Viable islet Cells from Dogs by a New Method

1981

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confidence: 99%

Preparation of Viable islet Cells from Dogs by a New Method

1981

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“…We used the liver in this series simply because we could not perserve the spleen in this model. The major complications like severe acute portal hypertension with venous pooling in the splanchnic regions, hypotension, disseminated intravascular coagulation (Mehigan et al 1980), documented in the implantation of pancreatic fragments by Mirkowitch's method (Mirkovitch and Campiche 1976) were never encoutered after implantation of about 1 ml of pure 500-600 islets into the liver from the portal vein.…”

Section: Discussionmentioning

confidence: 99%

Effect of islet transplantation on diabetes mellitus after subtotal pancreatectomy in rat: A quantitative analysis of islet tissue.

Yamauchi¹,

Kakizaki

et al. 1985

Tohoku J. Exp. Med.

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A reproducible model of diabetic state was produced by resecting more than 90% of the pancreatic tissue in Wistar strain male rats. Five rats with diabetic state after the resection received islet transplantation into the liver through the portal vein. All these five rats showed normoglycemia one week after the injection and it lasted about six months. A morphometric analysis using 1190 serial sections of the control rat pancreas showed that the islet distribution accorded satisfactorily with Weibull and gamma distribution, when an islet was regarded as a sphere in the three dimentional space. The estimated value of radius and number of the islets was 0.0155 mm and 131.2/mm3, respecitively, whereas volume fractions of the islet tissue in the splenic and gastric segments, and parabiliary and duodenal segments were estimated at 1.45 and 0.98%, respectively. The mean half of the largest diameter of the isolated islets under dissecting microscope reached to 0.080 mm and 500 isolated islets account for about 14% of volume fraction in a whole pancreas. As the remnant pancreas possibly had approximately five percent of islet volume, the transplanted animal contained about 19% of islet volume as compared to the control animal. It could be concluded that transplantation of 500 islets controlled diabetes in the rat with subtotal pancreatectomy. But the transplanted rats eventually became diabetic about six months after the transplantation possibly because of insufficient volume of implanted islets. islet transplantation ; subtotal Pancreatectomy ; islet morphometrySince establishment of a technique of pancreatic islet purification by Lacy

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“…The problem of poor recovery of islets from a large animal dissolved by Mirkovitch and Campiche (1976). They reported There were no fragments of pancreatic exocrine tissue.…”

Section: Discussionmentioning

confidence: 99%

“…However, autotransplantation in the experimental animals with chronic pancreatitis (Mehigan et al 1980) have generally brought about little beneficial effect mainly because of an inadequate procurement of islet tissue from the scarred pancreas, although presently the experimental autograft from the normal pancreas can control the diabetic state following total pancreatectomy (Mirkovitch and Campiche 1976;Kretschmer et al 1978;Mehigan et al 1980). Similarly in patients with chronic pancreatitis, it is difficult to make islet tissue preparations from the diseased pancreas, and islet autotranstplantation played only a limited role in controlling diabetes after total or near total pancreatectomy (Naj arian et al 1980; Traverso et al 1981).…”

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confidence: 99%