Successful Isolation of Viable Adipose-Derived Stem Cells from Human Adipose Tissue Subject to Long-Term Cryopreservation: Positive Implications for Adult Stem Cell-Based Therapeutics in Patients of Advanced Age

Devitt, Sean; Carter, Cynthia M.; Dierov, Raia; Weiss, Scott L.; Gersch, Robert P.; Percec, Ivona

doi:10.1155/2015/146421

Cited by 35 publications

(37 citation statements)

References 34 publications

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“…These results suggest a shortening of the G1 phase in aging ASCs, indicating a faster progression to subsequent cell cycle phases and cell proliferation. These subtle but important age‐related cell cycle modifications are consistent with our prior data supporting robust viability and proliferative capacities specific to aging ASCs that are not present in aging differentiated cells .…”

Section: Resultssupporting

confidence: 90%

“…ASCs were isolated from 20 tissue samples of female individuals between 24 and 64 years, according to a standard collagenase protocol . The isolated ASCs were cultured in Dulbecco's modified Eagle's medium/F12 (Gibco Life Technologies Co., Norwalk, CT, https://www.thermofisher.com/us/en/home/brands/gibco.html) supplemented with 1% Penicillin/Streptomycin (Gibco Life Technologies Co.) and 10% fetal bovine serum (Serum Source International, Charlotte, NC, http://www.serumsourceintl.com/) at 37°C with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

“…First, ASCs can be easily isolated with high yield from subcutaneous adipose tissue and are readily expandable by in vitro culturing. Second, ASCs consistently maintain their concentration and proliferation rates with advancing age . Finally, ASCs represent a more biologically relevant model with which to study the natural chronological mechanisms of aging, as compared to fast replicating stem cells that age largely by replicative exhaustion and senescence.…”

Section: Introductionmentioning

confidence: 97%

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Transcriptional and Cell Cycle Alterations Mark Aging of Primary Human Adipose-Derived Stem Cells

et al. 2017

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Adult stem cells play a critical role in the maintenance of tissue homeostasis and prevention of aging. While the regenerative potential of stem cells with low cellular turnover, such as adipose-derived stem cells (ASC), is increasingly recognized, the study of chronological aging in ASCs is technically difficult, and remains poorly understood. Here, we employ our model of chronological aging in primary human ASCs to examine genome-wide transcriptional networks. We demonstrate first that the transcriptome of aging ASCs is distinctly more stable than that of age-matched fibroblasts, and further, that age-dependent modifications in cell cycle progression and translation initiation specifically characterize aging ASCs in conjunction with increased nascent protein synthesis and a distinctly shortened G1 phase. Our results reveal novel chronological aging mechanisms in ASCs that are inherently different from differentiated cells, and that may reflect an organismal attempt to meet the increased demands of tissue and organ homeostasis during aging.

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Section: Resultssupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Transcriptional and Cell Cycle Alterations Mark Aging of Primary Human Adipose-Derived Stem Cells

et al. 2017

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“…The main focus is on the reduction of the cytotoxic cryoprotectant DMSO [51] and a xenofree and chemically defined protocol [52]. Also the cryopreservation of adipose tissue for more than 2 years was successfully demonstrated [53]. …”

Section: Mesodermal Potential Of Adipose Tissue-derived Stem Cellsmentioning

confidence: 99%

Adipose Tissue-Derived Stem Cells in Regenerative Medicine

Frese

Dijkman

Hoerstrup

2016

Transfus Med Hemother

353

245

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In regenerative medicine, adult stem cells are the most promising cell types for cell-based therapies. As a new source for multipotent stem cells, human adipose tissue has been introduced. These so called adipose tissue-derived stem cells (ADSCs) are considered to be ideal for application in regenerative therapies. Their main advantage over mesenchymal stem cells derived from other sources, e.g. from bone marrow, is that they can be easily and repeatable harvested using minimally invasive techniques with low morbidity. ADSCs are multipotent and can differentiate into various cell types of the tri-germ lineages, including e.g. osteocytes, adipocytes, neural cells, vascular endothelial cells, cardiomyocytes, pancreatic β-cells, and hepatocytes. Interestingly, ADSCs are characterized by immunosuppressive properties and low immunogenicity. Their secretion of trophic factors enforces the therapeutic and regenerative outcome in a wide range of applications. Taken together, these particular attributes of ADSCs make them highly relevant for clinical applications. Consequently, the therapeutic potential of ADSCs is enormous. Therefore, this review will provide a brief overview of the possible therapeutic applications of ADSCs with regard to their differentiation potential into the tri-germ lineages. Moreover, the relevant advancements made in the field, regulatory aspects as well as other challenges and obstacles will be highlighted.

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“…Since donor tissue is not always available on demand, strategies for the long term storage of human adipose tissue are required. The cryopreservation of liposuction aspirates from adipose tissue and isolation of MSCs by enzymatic treatment from long term cryopreserved adipose tissue was demonstrated before 31,32 . However, we present an approach for the cryopreservation of whole adipose tissue portions for isolation of MSCs by explant cultivation.…”

Section: Introductionmentioning

confidence: 99%

From 3D to 3D: isolation of mesenchymal stem/stromal cells into a three-dimensional human platelet lysate matrix

Egger

Oliveira

Mallinger

et al. 2019

Preprint

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Mesenchymal stem/stromal cells (MSCs) are considered an important candidate in cell therapy and tissue engineering approaches. The culture of stem cells in a 3D environment is known to better resemble the in vivo situation and to promote therapeutically relevant effects in isolated cells. Therefore, the aim of this study was to develop an approach for the isolation of MSCs from adipose tissue into a 3D environment. Furthermore, the use of cryoprotective medium for cryopreservation of whole adipose tissue was evaluated. For the isolation of MSCs, a novel human platelet lysate-based hydrogel was used as matrix and the migration, yield, viability and metabolic activity of cells from the 3D matrix were compared to cells from 2D explant culture. Also, the surface marker profile and differentiation capacity of MSCs from the 3D matrix were evaluated and compared to MSCs from isolation by enzymatic treatment. We found that cryopreservation of whole adipose tissue is feasible, and therefore adipose tissue can be stored and is available for MSC isolation on demand. Also, we demonstrate the isolation of MSCs into the 3D matrix and that cells from this condition display a similar phenotype and differentiation capacity like MSCs derived by traditional isolation procedure. The presented approach allows, for the first time, to isolate MSCs directly into a soft 3D hydrogel environment, avoiding any contact to a 2D plastic culture surface. 3D explant isolation of MSCs 3 Significance Statement:In this paper we present a new method for the isolation of mesenchymal stem cells. Usually, these cells grow on two-dimensional plastic surfaces which is far away from their physiologic environment. Our new method allows for the first time the direct outgrowth of cells from primary tissue into a three-dimensional environment, avoiding any contact to a two-dimensional plastic surface. In future, this will allow an entirely three-dimensional in vitro cultivation of stem cells.Using 3D isolated cells will probably also increase the physiologic relevance of in vitro models. 3D explant isolation of MSCs4

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Successful Isolation of Viable Adipose-Derived Stem Cells from Human Adipose Tissue Subject to Long-Term Cryopreservation: Positive Implications for Adult Stem Cell-Based Therapeutics in Patients of Advanced Age

Cited by 35 publications

References 34 publications

Transcriptional and Cell Cycle Alterations Mark Aging of Primary Human Adipose-Derived Stem Cells

Transcriptional and Cell Cycle Alterations Mark Aging of Primary Human Adipose-Derived Stem Cells

Adipose Tissue-Derived Stem Cells in Regenerative Medicine

From 3D to 3D: isolation of mesenchymal stem/stromal cells into a three-dimensional human platelet lysate matrix

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