Successful pregnancy and childbirth after intracytoplasmic sperm injection with calcium ionophore oocyte activation in a globozoospermic patient

Tejera, A.; Mollá, Marta; Muriel, Lourdes; Remohı́, José; Pellicer, António; Pablo, J.L. De

doi:10.1016/j.fertnstert.2007.11.056

Cited by 73 publications

(46 citation statements)

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“…Although previous investigations could determine DNA fragmentation level in very few patients (Table 2), in terms of quantity, this is the largest study in fully globozoospermic men, performed to find out any correlation between DNA damage level and CMA3 positive sperm cells with round head. Several studies were so far able to evaluate DNA damage level in only one patient, using different techniques including SCSA and comet assay (Larson et al, 2001), TUNEL assay (Vicari et al, 2002;Taylor et al, 2010;Perrin et al, 2011b;Sermondade et al, 2011), SCD (Tejera et al, 2008), and AOT (Egashira et al, 2009). Compared with those of fertile controls in Larson's and Sermondade's studies, further investigations on two and six *Correction added on 26 May 2015, after first online publication: sentence structure corrected.…”

Section: Discussionmentioning

confidence: 99%

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Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

et al. 2015

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SUMMARYThe absence of the acrosome causes the situation which is called globozoospermia. There are a few studies, mostly as case reports, about correlation between levels of sperm DNA damage in patients with total round-headed spermatozoa. We investigated this correlation as well as CMA3 positive spermatozoa in 20 globozoospermic men (with more than 90% round-headed spermatozoa) attending to Royan Institute. Semen samples divided into three parts to semen analysis, to measure DNA fragmentation index (DFI) using sperm chromatin structure assay (SCSA) and to detect CMA3 + sperm cells by chromomycin A3 staining and fluorescent microscopy. Our results showed that there were significant differences in sperm concentration, total sperm motility, and normal morphology between patients and controls group (p < 0.001). Moreover, the average of DFI and CMA3 positive spermatozoa in patients group significantly increases compared with control group (p < 0.001). A significant correlation between DFI and CMA3 + in total population was also detected in patients group (r = 0.45, p = 0.046). To our knowledge, this is the largest study about correlation between DNA damage levels and CMA3 positive spermatozoa with round head sperm cells in total globozoospermic men. It seems that the increase in DNA damage may be because of defective sperm DNA compaction, as we detected CMA3 positive sperm cells in these patients.

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Section: Discussionmentioning

confidence: 99%

“…Although, there have been some studies about correlation between round head spermatozoa and high levels of sperm DNA damage and protamine deficiency (Tejera et al, 2008;Egashira et al, 2009;Brahem et al, 2011;Sermondade et al, 2011), these reports were in limited number of patients, mostly in a case report.…”

Section: Introductionmentioning

confidence: 99%

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

et al. 2015

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“…Although ICSI bypasses zona pellucida crossing, oocyte activation does not occur with these deficient sperm, and this lack of activation seems to be correlated with PLC absence. Recent reports suggest that reduced amounts or abnormal forms of PLC could be overcome by activating the sperm-injected oocytes with Ca ionophore (Kyono et al, 2008, Tejera et al, 2008, Taylor et al, 2010. Although this treatment could lead to high rates of fertilization and an ongoing pregnancy, we may wonder what the risks are from both a probable abnormal Ca signal as already discussed above and a highly abnormal sperm material since a high percentage of sperm DNA fragmentation is associated with this pathology.…”

Section: Modifications In the Number And Period Of Ca I Oscillationsmentioning

confidence: 99%

Could modifications of signalling pathways activated after ICSI induce a potential risk of epigenetic defects?

Ciapa

Arnoult²

2011

Int. J. Dev. Biol.

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A calcium signal during oocyte or egg activation is a conserved event in virtually all species analyzed so far. This signal, that is in the form of calcium oscillations in mammals, is spatially and temporally controlled and is mainly supported by calcium release from internal calcium stores, but how it is triggered after fertilization is far from understood. The sperm factor hypothesis of egg activation postulates that sperm delivers a calcium-releasing factor into the egg following sperm-egg fusion. Among the many potential sperm factors, PLCzeta is the strongest bona fide sperm factor candidate. However, how sperm-oocyte fusion occurs prior to PLCzeta delivery and oocyte activation is not entirely known. We propose in the first part of this review the possibility that other pathways such as those involving G-proteins, tyrosine kinases or integrins could be activated besides sperm factor injection and could be upstream mechanisms involved in later embryonic development. Among different assisted reproductive technologies (ARTs), intracytoplasmic sperm injection (ICSI) is considered as the best and easiest therapeutic technique to circumvent severe male infertility. Although most reports are reassuring, some recent data suggest a greater incidence of abnormalities in children conceived by ART compared with those conceived normally. Spatio-temporal signals may be missing or abnormal during ICSI, perhaps because membrane fusion and signalling events are bypassed. We discuss in the second part of this review the hypothesis that potential perturbations during the ICSI procedure may have repercussions on epigenetic processes, inducing not only alterations of embryonic development, but also diseases in young children and, perhaps, in adults. KEY WORDS: fertilization, oocyte, egg, ICSI, calcium, epigenetics Activation of the mammalian egg after fertilizationMany reviews have been published to describe how adhesion and fusion of sperm and egg at fertilization induces the formation of a zygote that develops into a new individual. In mammals, the oocyte that can be fertilized is arrested at metaphase of second meiotic division after ovulation. The metabolism of this oocyte is low, and if no fertilization occurs the oocyte will die. This arrest of the cell cycle is due to high activities of MAPK ("M-phase activating protein kinase") and MPF ("mitosis promoting factor"), the latter being a complex between cdc2 kinase and cyclin B. After fertilization, oocyte activation triggers inactivation of these two kinases, exit of meiosis, and start of embryonic development (Ducibella and Fissore, 2008). Normally, egg activation after fertilization starts from the point of sperm-egg interaction and Int.

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“…Moreover, successful fertilization and pregnancy following AOA-globozoospermic ICSI has been reported in a few case reports (Tesarik et al, 2002;Tejera et al, 2008;Karaca et al, 2014;Chansel-Debordeaux et al, 2015). Unlike other studies, Zhang et al (2015) described a successful pregnancy without assisted activation in a couple with partial globozoospermia.…”

Section: Introductionmentioning

confidence: 99%

Expression profile ofPLCζ,PAWP,andTR-KITin association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation

Tavalaee

Nasr-Esfahani

2016

Andrology

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SUMMARYSperm-mediated oocyte activation critically depends upon appropriate expression and assembly of sperm-borne oocyte-activating factors (SOAFs) during spermiogenesis. Several factors have been considered as candidate for SOAFs over the recent years. However, little is known about the expression profile of these candidates and their potential contribution to the clinical outcomes of intra-cytoplasmic sperm injection (ICSI), particularly in globozoospermia. This study investigated the expression profile of PLCf, PAWP, and TR-KIT and clinical outcomes of ICSI in 12 men with total globozoospermia and compared with 12 fertile individuals. Levels of PLCf, PAWP, and TR-KIT mRNA in the spermatozoa of fertile men were significantly higher than the corresponding values of the globozoospermic subjects. Interestingly, at protein level, expressions of these factors in the cases assessed were low in globozoospermic individuals. Fertilization rates following artificial oocyte activation (AOA) in the majority of globozoospermic couples were higher than the expected 30% cut-off value reported for individuals with failed or low fertilization rate. Clinical outcomes of ICSI-AOA were dependent on inter-individual variation in globozoospermic couples. None of the SOAFs assessed could provide a greater prediction value with respect to fertilization rate in globozoospermic couples which underwent ICSI-AOA. High fertilization (56.06%) and pregnancy (41.7%) rates accomplished in this study following ICSI-AOA indicated that expression profiles of PLCf, PAWP, and TR-KIT were low in globozoospermic individuals, and ICSI combined with artificial oocyte activation could mimic physiological calcium changes which occur during fertilization.

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Successful pregnancy and childbirth after intracytoplasmic sperm injection with calcium ionophore oocyte activation in a globozoospermic patient

Cited by 73 publications

References 45 publications

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

Could modifications of signalling pathways activated after ICSI induce a potential risk of epigenetic defects?

Expression profile ofPLCζ,PAWP,andTR-KITin association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation

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