Successful pregnancy and delivery after transfer of a single blastocyst derived from a vitrified mature human oocyte

Kyono, Koichi; Fuchinoue, Kohei; Yagi, Akiko; Nakajo, Yukiko; Yamashita, Ayako; Kumagai, Shinya

doi:10.1016/j.fertnstert.2005.04.051

Cited by 50 publications

(24 citation statements)

References 10 publications

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“…Therefore, in our IVF center, we do not routinely freeze immotile sperm from testicular biopsy samples. It remains to be confirmed whether or not testicular tissues from diagnostic biopsy should be frozen as a back up for NOA patients in case sperm cannot be retrieved on the day of oocyte retrieval [Kyono et al 2005;Chen et al 2008].…”

Section: Discussionmentioning

confidence: 99%

“…However in some countries, like China, the use of donor sperm is impossible immediately requiring other options like, emergency oocyte cryopreservation until sperm can be recovered from the husband. To date, the information available for emergency oocyte cryopreservation is very limited [Emery et al 2004;Kyono et al 2005]. The objective of this study is to report the pregnancy and obstetric outcomes in 15 women following the use of emergency oocyte vitrification.…”

Section: Introductionmentioning

confidence: 99%

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Clinical outcome of emergency egg vitrification for women when sperm extraction from the testicular tissues of the male partner is not successful

Song

Sun

Jin

et al. 2011

Systems Biology in Reproductive Medicine

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The development of an effective oocyte cryopreservation system will have a significant impact on the clinical practice of reproductive medicine. However, the important option of emergency oocyte cryopreservation has yet to be well documented. In this report, we review the cases of 15 women with male partners who were diagnosed with nonobstructive azoospermia and for whom testicular sperm extraction on the day of oocyte retrieval failed. Emergency oocyte vitrification was performed and after two months, the vitrified oocytes were warmed and the surviving oocytes inseminated with frozen-thawed donor sperm by intracytoplasmic sperm injection (ICSI). A total of 117 mature oocytes from the 15 women were vitrified and warmed. The post-warming survival rate was 84.6% (99/117), and the fertilization rate following ICSI was 83.8% (83/99). We selected 30 embryos for transfer to 15 patients, 8 of whom became pregnant. The clinical pregnancy rate was 53.3% (8/15) and the implantation rate was 30.0% (9/30). Nine healthy live births resulted from 8 pregnancies. These results indicate that emergency oocyte vitrification is an effective rescue technique that can be applied clinically with acceptable pregnancy and live birth rates when testicular sperm extraction from the male partner failed on the day of oocyte retrieval. These results also highlight another important option for oocyte cryopreservation through the use of vitrification technology.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Clinical outcome of emergency egg vitrification for women when sperm extraction from the testicular tissues of the male partner is not successful

Song

Sun

Jin

et al. 2011

Systems Biology in Reproductive Medicine

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“…Alternatively, oocytes can be cryopreserved [27] in the anticipation of medical advances (i.e., in vitro culture).…”

Section: Discussionmentioning

confidence: 99%

Seven pregnancies and deliveries from non-mosaic Klinefelter syndrome patients using fresh and frozen testicular sperm

Kyono

Uto

Nakajo

et al. 2006

J Assist Reprod Genet

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Purpose: The aim of this study was to investigate the feasibility of using frozen-thawed testicular sperm as well as the timing of testicular sperm extraction (TESE) in patients with non-mosaic Klinefelter syndrome.Methods: Intracytoplasmic sperm injection (ICSI) was performed in six of 17 (35%) patients whose sperm was recovered by TESE. Multiple biopsies of both testes were performed on the day of oocyte retrieval in all but one of the six patients.Results: Seven pregnancies and deliveries were achieved in five couples, and one couple was unsuccessful. Five pregnancies were achieved using fresh motile sperm, and two were achieved using frozen-thawed sperm. Sperm cryopreservation was not possible in one of the five couples because of the small number of recovered sperm, and possible in four other couples for subsequent ICSI. One woman whose husband had TESE performed prior to ovarian stimulation did not become pregnant. This may be due to the attainment Conclusion: The outcome of ICSI using fresh or frozenthawed testicular sperm in patients with non-mosaic Klinefelter syndrome was identical; however, TESE should be performed on the day of oocyte retrieval until such time as a procedure with a higher sperm yield from TESE is available. Moreover, an improved recovery procedure after cryopreservation-thawing of a single spermatozoon must be developed.

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“…Two blastocysts on day 5 (grade 4BC, 5BA (Fig. 1)) and one blastocyst on day 6 (grade 4BC) [2] were vitrified on a cryotop [3]. Two months later, 6 mg per day of oral estradiol (estradiol tablets, USP; Mylan Pharmaceuticals, Morgantown, USA) was administered from day 1, and 50 mg per day of progesterone (Fuji Pharmaceutical Co., Ltd., Tokyo, Japan) was administered from day 14 (blood E2 level, 415.7 pg/ml; blood progesterone level, 0.2 ng/ml; maximum follicular diameter of both ovaries, less than 10 mm; endometrial thickness, 9.1 mm).…”

Section: Casementioning

confidence: 99%

A successful birth of healthy monozygotic dichorionic diamniotic (DD) twins of the same gender following a single vitrified-warmed blastocyst transfer

Shibuya

Kyono

2012

J Assist Reprod Genet

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Purpose To report the world's first case of live monozygotic dichorionic diamniotic (DD) twins after a single vitrifiedwarmed blastocyst transfer in a cycle during which hormone supplements were given. Methods and results The patient was a 39-year-old woman with tubal factor infertility. Six oocytes were retrieved and three blastocysts were vitrified after insemination with her husband's sperm. Two months later, a single warmed blastocyst was transferred on the fifth day after progesterone supplementation during the luteal phase. Two gestational sacs and fetuses with heartbeats showing lambda sign under ultrasonography were recognized at 7 weeks of gestation and the patient gave birth to two female infants at gestational 33 weeks. The pathological examination demonstrated that the DD twin placentas fused in the third trimester. Conclusions This report suggests that the splitting of the transferred embryo took place after the blastocyst stage and the popular credo that the timing of embryo division governs the placental configuration of monozygotic twins (MZT) must be re-examined as to its veracity.

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Successful pregnancy and delivery after transfer of a single blastocyst derived from a vitrified mature human oocyte

Cited by 50 publications

References 10 publications

Clinical outcome of emergency egg vitrification for women when sperm extraction from the testicular tissues of the male partner is not successful

Clinical outcome of emergency egg vitrification for women when sperm extraction from the testicular tissues of the male partner is not successful

Seven pregnancies and deliveries from non-mosaic Klinefelter syndrome patients using fresh and frozen testicular sperm

A successful birth of healthy monozygotic dichorionic diamniotic (DD) twins of the same gender following a single vitrified-warmed blastocyst transfer

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