Successful preservation of capercaillie (Tetrao urogallus L.) semen in liquid and frozen states

Kowalczyk, Artur; Łukaszewicz, Ewa; Rzońca, Zenon

doi:10.1016/j.theriogenology.2011.09.015

Cited by 23 publications

(36 citation statements)

References 28 publications

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“…In the presented experiment, one male (no 72) of four examined had a low SQF (8.6 on average), mainly due to low number of live normal sperm (33.3%). The ejaculates produced by the remained males were at a high level (SQF from 40.4 to 74.3), comparable to those presented in our previous publications (Kowalczyk & Łukaszewicz, ; Kowalczyk et al., ).…”

Section: Discussionsupporting

confidence: 90%

“…The decrease of live, morphologically normal spermatozoa with simultaneous increase in deformed ones, has been described in roosted semen stored in vitro up to 24 h at 4°C (Blesbois et al., ; Siudzińska & Łukaszewicz, ), but our earlier experiment carried out on capercaillie semen (Kowalczyk et al., ) indicated, similarly as in the presented one, that changes observed in spermatozoa morphology were not significant. It might suggest that capercaillie spermatozoa are more resistant to lipid peroxidation and antioxidant imbalance caused by the oxidative stress occurring during in vitro liquid semen storage than other Galliformes species.…”

Section: Discussionsupporting

confidence: 57%

“…Kowalczyk et al. () and Ciereszko et al. (), using basic EK diluent to store capercaillie semen for 24 hr, did not find its negative effect on sperm motility.…”

Section: Discussionmentioning

confidence: 95%

“…We documented that in case of capercaillie, for successful AI purposes, the ejaculate quality has to be at minimum 10 SQF. Males whose ejaculates are scored at 10 SQF and more can be recognized as sufficiently good semen donors (Kowalczyk & Łukaszewicz, ; Kowalczyk et al., ; Łukaszewicz & Kowalczyk, ). In the presented experiment, one male (no 72) of four examined had a low SQF (8.6 on average), mainly due to low number of live normal sperm (33.3%).…”

Section: Discussionmentioning

confidence: 99%

“…By the Decree of Polish Ministry of the Environment (“The protection of animal species,” October 12, 2011), the capercaillie ex situ in vivo breeding centres and reintroduction programmes were established (Merta, Kobielski, Krzywiński, & Rzońca, ; Rzońca, Łukaszewicz, & Kowalczyk, ; Strzała, Kowalczyk, & Łukaszewicz, ). An increasing the reproductive efficiency of captive kept capercaillie by the use of biotechnological methods contributes significantly to the achievement of this goal (Ciereszko, Dietrich, Liszewska, Krzywiński, & Kobus, ; Kowalczyk & Łukaszewicz, ; Kowalczyk, Łukaszewicz, & Rzońca, ; Łukaszewicz & Kowalczyk, ; Łukaszewicz, Kowalczyk, & Rzońca, ). The possibility of short‐term semen storage will expand the applicability of assisted reproduction technology in capercaillie breeding centres.…”

Section: Introductionmentioning

confidence: 99%

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Effect of selenium and vitamin E addition to the extender on liquid stored capercaillie (Tetrao urogallus) semen quality

Kowalczyk

Klećkowska‐Nawrot

Łukaszewicz

2017

Reprod Domestic Animals

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Captive breeding has become an important tool in species conservations programmes, maintaining genetic diversity and restoring wild, endangered populations. In order to improve the reproductive efficiency of captive kept capercaillie, the purpose of the study was to determine the effect of selenium and vitamin E addition to semen extender on sperm characteristic during short-term storage. Ejaculates collected individually from four capercaillie were divided into two parts, diluted threefold with basic EK extender and EK enriched with 1 mg/ml of organic selenium and 8 mg/ml of vitamin E (EK+Se+E) and stored 24 hr at temp. +4°C. Spermatozoa morphology, motility and motility parameter were evaluated in net, diluted and stored semen samples. Significant (p < .05) differences between individual males were stated in relation to the majority of traits evaluated in the freshly collected semen. Comparing to the fresh semen, a significant (p < .05) decrease in percentage of live sperm in total (by 3.8% points on average) has been observed in samples diluted by EK extender, while in semen diluted with EK+Se+E extender this decrease was lower (1.5%pts on average) and not significant. Also per cent of motile sperm in EK+Se+E extender was higher (p < .05) then in EK (71.6% vs. 58.9%), but taking into account the values of individual males, both extender and male effect on liquid semen storage become apparent. Obtained data allow concluding that selenium and vitamin E addition to EK extender had positive effect on morphology and motility of capercaillie semen stored 24 hr at 4°C and can be recommended for similar studies carried out on other Galliformes species.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 57%

“…Kowalczyk et al. () and Ciereszko et al. (), using basic EK diluent to store capercaillie semen for 24 hr, did not find its negative effect on sperm motility.…”

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of selenium and vitamin E addition to the extender on liquid stored capercaillie (Tetrao urogallus) semen quality

Kowalczyk

Klećkowska‐Nawrot

Łukaszewicz

2017

Reprod Domestic Animals

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Use of native chicken breeds (Gallus gallus domesticus) for the development of suitable methods of Cantabrian capercaillie (Tetrao urogallus cantabricus) semen cryopreservation

O’Brien

Castaño

Toledano-Dı́az

et al. 2022

Veterinary Medicine & Sci

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Background:The Cantabrian capercaillie (Tetrao urogallus cantabricus) is critically endangered. This subspecies has the lowest genetic variability and it is in regression. It belongs to Phasianidae family; therefore, the domestic chicken (Gallus gallus domesticus) could be a good model for developing reproductive technologies for use in capercaillie populations with low availability of animals.Objectives: In this study, we analyzed the response of capercaillie sperm to the freezing-thawing process for contributing to the development of a semen cryobank of Cantabrian capercaillie. Methods:We used domestic chicken as the animal model in order to obtain the freezing protocol before applying on capercaillie. In the first experiment, two different extenders (EK and LR84) and different concentrations [4% and 6% dimethyl-acetamide (DMA) v:v] of cryoprotectants were evaluated using in-straw freezing method in domestic chickens. A pilot study in capercaillie males, using the same conditions evaluated in chicken, was performed. Results:In chicken, we found that the LR84-4% DMA media provided the best results for freezing semen. In capercaillie study, LR84 extender seemed to be the most appropriate diluent and 4% was the better dose of DMA cryoprotectant agent. Further, based on previous studies carried out in rooster samples, we also tested the glycerol (8% v/v) as a cryoprotectant for capercaillie semen cryopreservation. Conclusions:Our results suggest that sperm from both domestic and wild species had a similar response to freezing-thawing processes. Mediterranean chickens may be used as a suitable model for developing sperm freezing protocols that can be extrapolated to threatened capercaillie populations. In addition, LR84 media with glycerol was the most efficient extender to freeze capercaillie sperm native.

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Successful use of artificial insemination in the production of red-legged partridges (Alectoris rufa)

Abouelezz¹,

Castaño²,

Toledano-Dı́az³

et al. 2015

Eur J Wildl Res

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Successful preservation of capercaillie (Tetrao urogallus L.) semen in liquid and frozen states

Cited by 23 publications

References 28 publications

Effect of selenium and vitamin E addition to the extender on liquid stored capercaillie (Tetrao urogallus) semen quality

Effect of selenium and vitamin E addition to the extender on liquid stored capercaillie (Tetrao urogallus) semen quality

Use of native chicken breeds (Gallus gallus domesticus) for the development of suitable methods of Cantabrian capercaillie (Tetrao urogallus cantabricus) semen cryopreservation

Successful use of artificial insemination in the production of red-legged partridges (Alectoris rufa)

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