Successful selective production of vanillic acid from depolymerized sulfite lignin and its application to poly(ethylene vanillate) synthesis

Higuchi, Y; Ishimaru, Hiroya; Yoshikawa, Takuya; Masuda, Toshio; Sakamoto, Chiho; Kamimura, Naofumi; Masai, Eiji; Takeuchi, Daisuke; Sonoki, Tomonori

doi:10.1016/j.biortech.2023.129450

Cited by 9 publications

(1 citation statement)

References 45 publications

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“…Lastly, with the development of synthetic biology, the production of high-value-added secondary metabolites by engineered microorganisms, defined as “natural” production, has become a more economical and environmentally friendly method, but it still has huge development prospects. For example, VA can be de novo synthesized from precursor protocatechuic acid (PCA), another important phenolic acid, which was developed as a conventional Chinese pharmaceutical for the treatment of bronchitis. − In addition, VA can be obtained via the biotransformation from lignin-derived monomers (including syringol, isoeugenol, ferulic acid, and vanillin) by engineered microorganisms. − However, the low yield, titer, and productivity of engineered strains have limited the development of industrial applications. Owing to a series of advantages, including short production cycle, simple separation and purification process, low costs, and ease of large-scale production, engineering microbes for VA production has been highlighted as an attractive candidate .…”

Section: Introductionmentioning

confidence: 99%

Metabolic Engineering of Pseudomonas putida KT2440 for De Novo Biosynthesis of Vanillic Acid

Li,

Fu,

Shang

et al. 2024

J. Agric. Food Chem.

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Vanillic acid (VA), as a plant-derived phenolic acid compound, has widespread applications and good market prospects. However, the traditional production process cannot meet market demand. In this study, Pseudomonas putida KT2440 was used for de novo biosynthesis of VA. Multiple metabolic engineering strategies were applied to construct these P. putida-based cell factories, including the introduction of a Hs-OMT opt , engineering the cofactor S-adenosylmethionine supply pathway through the overexpression of metX and metH, reforming solubility of Hs-OMT opt , increasing a second copy of Hs-OMT opt , and the optimization of the fermentation medium. The resulting strain, XCS17, de novo biosynthesized 5.4 g/L VA from glucose in a fed-batch fermentation system; this is the highest VA production titer reported up to recently. This study showed that P. putida KT2440 is a robust platform for achieving the effective production of phenolic acids.

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