Successful use of <i>Nicotiana tabacum</i> hairy roots for the recombinant production of Cecropin A peptide

Hashemi, Seyed Yaser; Niazi‎, Ali; Baghizadeh, Amin; Taghizadeh, Mohammad Sadegh

doi:10.1002/bab.2158

Cited by 12 publications

(5 citation statements)

References 29 publications

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“…However, our findings indicate that desired peptides are excellent candidates for developing new natural food preservative or drugs in the treatment of bacterial or cancerous diseases and will open new avenues for designing more selective and effective drugs. Furthermore, these peptides could recombinantly be produced in the plant expression systems to use as a fresh functional food [ 51 , 52 ].…”

Section: Resultsmentioning

confidence: 99%

In vitro and in silico studies for the identification of anti-cancer and antibacterial peptides from camel milk protein hydrolysates

2023

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Today, breast cancer and infectious diseases are very worrying that led to a widespread effort by researchers to discover natural remedies with no side effects to fight them. In the present study, we isolated camel milk protein fractions, casein and whey proteins, and hydrolyzed them using pepsin, trypsin, and both enzymes. Screening of peptides with anti-breast cancer and antibacterial activity against pathogens was performed. Peptides derived from whey protein fraction with the use of both enzymes showed very good activity against MCF-7 breast cancer with cell viability of 7.13%. The separate use of trypsin and pepsin to digest whey protein fraction yielded peptides with high antibacterial activity against S. aureus (inhibition zone of 4.17 ± 0.30 and 4.23 ± 0.32 cm, respectively) and E. coli (inhibition zone of 4.03 ± 0.15 and 4.03 ± 0.05 cm, respectively). Notably, in order to identify the effective peptides in camel milk, its protein sequences were retrieved and enzymatically digested in silico. Peptides that showed both anticancer and antibacterial properties and the highest stability in intestinal conditions were selected for the next step. Molecular interaction analysis was performed on specific receptors associated with breast cancer and/or antibacterial activity using molecular docking. The results showed that P3 (WNHIKRYF) and P5 (WSVGH) peptides had low binding energy and inhibition constant so that they specifically occupied active sites of protein targets. Our results introduced two peptide-drug candidates and new natural food additive that can be delivered to further animal and clinical trials.

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Section: Resultsmentioning

confidence: 99%

In vitro and in silico studies for the identification of anti-cancer and antibacterial peptides from camel milk protein hydrolysates

2023

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“…The advancement in molecular farming has also led to the development of plant cell suspension cultures, extendable to include other clonally propagated platforms in containment, including plant organ cultures like hairy roots, which have been explored for AMP production (Chahardoli et al ., 2018; Hashemi et al ., 2022; Khademi et al ., 2019; Shams et al ., 2019). Most importantly, the platform can integrate good manufacturing practices from the pharmaceutical industry and is scalable for large‐scale production.…”

Section: Molecular Farming Of Ampsmentioning

confidence: 99%

Molecular farming for sustainable production of clinical‐grade antimicrobial peptides

Chaudhary,

Ali,

Mahfouz

2024

Plant Biotechnology Journal

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SummaryAntimicrobial peptides (AMPs) are emerging as next‐generation therapeutics due to their broad‐spectrum activity against drug‐resistant bacterial strains and their ability to eradicate biofilms, modulate immune responses, exert anti‐inflammatory effects and improve disease management. They are produced through solid‐phase peptide synthesis or in bacterial or yeast cells. Molecular farming, i.e. the production of biologics in plants, offers a low‐cost, non‐toxic, scalable and simple alternative platform to produce AMPs at a sustainable cost. In this review, we discuss the advantages of molecular farming for producing clinical‐grade AMPs, advances in expression and purification systems and the cost advantage for industrial‐scale production. We further review how ‘green’ production is filling the sustainability gap, streamlining patent and regulatory approvals and enabling successful clinical translations that demonstrate the future potential of AMPs produced by molecular farming. Finally, we discuss the regulatory challenges that need to be addressed to fully realize the potential of molecular farming‐based AMP production for therapeutics.

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“…2). Among all selected papers, that strain produced the greatest induction rate (90%; [37,57]), high transformation e ciency (67%; [24]), and short root-drop time (5 days; [47]), in addition to advantages for root growth [7]. Nevertheless, other R. rhizogenes lines perform as well as ATCC 15834, including LBA 9402 and A4 [40].…”

Section: Transformation Proceduresmentioning

confidence: 99%

Nicotiana hairy roots for recombinant protein expression, where to start? A systematic review

et al. 2023

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Hairy roots are a plant-tissue culture raised by Rhizobium rhizogenes infection (formerly known as Agrobacterium rhizogenes). Nowadays, these roots have been gaining more space in biotechnology due to their bene ts for the recombinant expression of valuables proteins; it includes simpli ed downstream processing, protein rhizosecretion, and scalability in bioreactors. However, due to methodological inconsistency among reports, the tissue platform is still a disruptive technology. In the current paper, we propose the rst step to overcome this issue through a systematic review of studies that employ Nicotiana hairy roots for recombinant expression. We conducted a qualitative synthesis of 36 out of 387 publications initially selected. Following the PRISMA procedure, all papers were assessed for exclusion and inclusion criteria. Multiple points of root culture were explored, including transformation methods, root growth curve, external additives, and scale-up with bioreactors to determine which approaches performed best and what is still required to achieve a robust protocol. The information presented here may help researchers who want to work with hairy roots in their laboratories trace a successful path to high recombinant expression.

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Successful use of Nicotiana tabacum hairy roots for the recombinant production of Cecropin A peptide

Cited by 12 publications

References 29 publications

In vitro and in silico studies for the identification of anti-cancer and antibacterial peptides from camel milk protein hydrolysates

In vitro and in silico studies for the identification of anti-cancer and antibacterial peptides from camel milk protein hydrolysates

Molecular farming for sustainable production of clinical‐grade antimicrobial peptides

Nicotiana hairy roots for recombinant protein expression, where to start? A systematic review

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