Succession of Microbial Community in Gray Forest Soil during the Decomposition of Different Organic Compounds

Khomutova, T. E.; Dushchanova, Kamilla; Смирнов, В. Э.; Борисов, А. В.

doi:10.1134/s1064229319080088

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…This is consistent with past SIP work (Franklin and Mills, 2006) including in aquatic systems where a wide variety of planktonic bacteria became more enriched with 13 C from amino acids than from glucose (Bryson et al ., 2017). It is also consistent with laboratory experiments of forest soil in which the succession of microbial communities during the decomposition of proteins, lipids and polysaccharides was studied (Khomutova et al ., 2019).…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic organization in the assimilation of chemically distinct substrates by soil bacteria

Dang

Walkup

Hungate

et al. 2021

Environmental Microbiology

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Soils are among the most biodiverse habitats on earth and while the species composition of microbial communities can influence decomposition rates and pathways, the functional significance of many microbial species and phylogenetic groups remains unknown. If bacteria exhibit phylogenetic organization in their function, this could enable ecologically meaningful classification of bacterial clades. Here, we show non-random phylogenetic organization in the rates of relative carbon assimilation for both rapidly mineralized substrates (amino acids and glucose) assimilated by many microbial taxa and slowly mineralized substrates (lipids and cellulose) assimilated by relatively few microbial taxa. When mapped onto bacterial phylogeny using ancestral character estimation this phylogenetic organization enabled the identification of clades involved in the decomposition of specific soil organic matter substrates. Phylogenetic organization in substrate assimilation could provide a basis for predicting the functional attributes of uncharacterized microbial taxa and understanding the significance of microbial community composition for soil organic matter decomposition.

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Section: Discussionmentioning

confidence: 99%

Phylogenetic organization in the assimilation of chemically distinct substrates by soil bacteria

Dang

Walkup

Hungate

et al. 2021

Environmental Microbiology

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“…The reaction of solutions of carboxylic acids and amino acids was brought to pH = 6-7 by adding 1 n NaOH or 1 n HCl. The MST analysis was performed similarly to C-SIR determination [23]. Weighted portions of each soil sample (1 g) were placed in tubes (15 mL) with plastic plugs and incubated during 12 hours at 22°C.…”

Section: Carbon Of Microbial Biomass Which Gives a Respiratory Respon...mentioning

confidence: 99%

“…It is proposed to use the ratio between respiratory responses to added amino acids and carboxylic acids to detect the presence of organic substances in gray forest and chestnut soils [23]. Soils on different elements of catenas significantly differ in the ratios between respiratory responses to glycine and succinic acid, alanine and ascorbic acid, and arginine and ascorbic acid (Fig.…”

Section: Forest Arable Landmentioning

confidence: 99%

Soil Microbial Biomass and Functional Diversity of Microbial Communities in Native and Arable Soils of the Belogor’e Reserve

et al. 2022

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Microbial biomass and functional diversity of soil microbial communities in forest soils of the Belogor'e Nature Reserve and in arable soils formed under similar geomorphologic and lithological conditions were determined. We have analyzed the content of carbon of total microbial biomass (C-TMB), of the biomass of living microorganisms by the content of soil phospholipids (C-PL.), and of biomass of microbial cells capable of glucose assimilation (C-SIR). The respiratory response of the soil microbial community to the introduction of different groups of organic compounds-inductors (amino acids and carboxylic acids) was assessed by the method of multisubstrate testing (MST). It is shown that anthropogenic transformation of natural ecosystems results in a decrease in the total microbial biomass, biomass of living cells, and cells that give a respiratory response to glucose. The functional diversity of microbial communities in soils in natural and transformed ecosystems significantly differs. Soil plowing has resulted in a significant decrease in the ability of the soil microbial community to assimilate low-molecular organic compounds. This is most typical for amino acids: arginine, alanine, and glycine (a 2.7-, 5.4-, and 7.1-time decrease, respectively, as compared to native soils). Among carboxylic acids, the decrease in the respiratory response to the introduction of succinic acid is the most pronounced (8.7 times). It has been revealed that the geomorphologic position in natural ecosystems does not affect the biomass of the microbial community and its functional diversity, while the arable soils are characterized by a clear tendency to an increase in these parameters on the lower part of the slope.

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Microbiological Approach to Reconstruction of the Original Content of Pots from the Burials

Khomutova

Dushchanova

Борисов

2020

NAV

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In a model soil experiment, microbial decomposition nature and its rate applying to various organic substrates were studied. It is shown that the decomposition of various materials changes the functional features of microbial communities. These changes are assessed using multisubstrate testing of respiratory activity of microbial communities – this testing assumes application of low- molecular respiratory inducers (aminoacids and carboxylic acids) to the soil and registration the changes of the intensity of carbon dioxide release by microorganisms. Indicative compounds such as ascorbate, lactate, acetate (from the group of carboxylic acid salts) and cysteine (from the group of aminoacids) have been identified to be promising for use in reconstructing the original contents of ritual vessels. As an example, the article presents the results of reconstruction of one of bronze age burial pots original content, obtained by multisubstrate testing. High microbial biomass in the bottom layer of the vessel and the specific microbial communities’ respiratory responses to carboxylic acids salts (ascorbate, lactate and acetate) and aminoacid cysteine addition, indicate that the vessel originally contained a nutritional product, the decomposition of which led both to increase of microbial biomass and to changes in the functional structure of microbial community in the soil filling of the bottom layer. The results of statistical analysis of the reactions of soil microbial communities from the pot and from soils of the model experiment with known materials decomposed indicate that the vessel initially contained a protein product with a possible component of oil and starch.

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Succession of Microbial Community in Gray Forest Soil during the Decomposition of Different Organic Compounds

Cited by 3 publications

References 7 publications

Phylogenetic organization in the assimilation of chemically distinct substrates by soil bacteria

Phylogenetic organization in the assimilation of chemically distinct substrates by soil bacteria

Soil Microbial Biomass and Functional Diversity of Microbial Communities in Native and Arable Soils of the Belogor’e Reserve

Microbiological Approach to Reconstruction of the Original Content of Pots from the Burials

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