Successive cycles of utilization of novozym 435 in three different reaction systems

Lerin, Lindomar Alberto; Ceni, Giovana Cristina; Richett, A.; Kubiak, Gabriela Busnello; Oliveira, J. Vladimir; Toniazzo, Geciane; Treichel, Helen; Oestreicher, Enrique G.; Oliveira, Débora de

doi:10.1590/s0104-66322011000200002

Cited by 31 publications

(23 citation statements)

References 20 publications

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“…The sub‐products of esterification/transesterification using TFMA, VMA and MA are 2,2,2‐trifluoroethanol, acetaldehyde and water, respectively. While 2,2,2‐trifluoroethanol and water can be easily removed from the reaction medium, acetaldehyde can poison the enzyme, thereby decreasing enzyme reusability …”

Section: Resultsmentioning

confidence: 99%

Enzymatic synthesis and structural characterization of methacryloyl‐D‐fructose‐ and methacryloyl‐D‐glucose‐based monomers and poly(methacryloyl‐D‐fructose)‐based hydrogels

Perin

Felisberti

2018

J of Chemical Tech & Biotech

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BACKGROUND Carbohydrates are important renewable raw materials and their modification by enzymatic reactions with polymerizable groups is of great importance due the possibility of production of polymers with properties for biomedical applications. In this study, D‐fructose‐ and D‐glucose‐based monomers were prepared by enzymatic reactions and structurally characterized. Moreover, hydrogels based on poly(methacryloyl‐D‐fructose) were also prepared and characterized. RESULTS The conversions of 99% and 34% for D‐fructose and D‐glucose, respectively, were achieved using 2,2,2‐trifluoroethyl methacrylate as the methacrylic donor and commercial lipase Novozyme® 435 (EC 3.1.1.3) as enzymatic catalyst in t‐butanol. The molar ratios of D‐fructose and D‐glucose mono‐ and dimethacrylate were 57/43 and 87/13, respectively. These monomers are an isomeric mixture related to the tautomeric equilibrium of the carbohydrates in solution. Methacryloyl‐D‐fructose was polymerized in the presence of a controlled amount of ethyleneglycol dimethacrylate as crosslinker achieving hydrogels with different crosslinking densities which are mechanically stable when subject to compression–decompression cycles and have a high water swelling capability. CONCLUSION The combination of 2,2,2‐trifluoroethylmethacrylate and Novozym® 435 in a heterogeneous media lead to the continuous carbohydrate solubilization and reaction with the formation of carbohydrates‐based monomers. The control of the monomer functionality enables the synthesis of hydrogels with different crosslinking densities that tune their properties. © 2017 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Enzymatic synthesis and structural characterization of methacryloyl‐D‐fructose‐ and methacryloyl‐D‐glucose‐based monomers and poly(methacryloyl‐D‐fructose)‐based hydrogels

Perin

Felisberti

2018

J of Chemical Tech & Biotech

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“…Lipase enzyme can act as biocatalyst in the hydrolysis of vegetable oil and it possesses chemoselectivity, regioselectivity and stereoselectivity (Lerin et al, 2011;Tan et al, 2004;Saxena et al, 2003). At oil-water interface, it hydrolyzes carboxyl ester bond to release fatty acids and organic alcohols (Pereira et al, 2003;Leal et al, 2002;Kamimura et al, 1999;Merçon et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Effects of process variables and additives on mustard oil hydrolysis by porcine pancreas lipase

Goswami

Basu

2012

Braz. J. Chem. Eng.

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-Selective hydrolysis of brown mustard oil (from Brassica juncea) with regioselective porcine pancreas lipase was studied in this work. Buffer and oil phase were considered as the continuous and dispersed phases, respectively. Effects of speed of agitation, pH of the buffer phase, temperature, buffer-oil ratio and enzyme concentration on hydrolysis were observed. The best combination of process variables was: 900 rpm, pH 9, 35 ºC, buffer-oil ratio of 1:1 and enzyme concentration of 10 mg/g oil. These standard conditions led to 50% hydrolysis and selective production of 55% erucic acid in 6 h. Cations like Mg 2+ and Ca 2+ increased hydrolysis, but Cu 2+ strongly inhibited it. Organic solvents decreased hydrolysis, though the decrease was minimum for isooctane. A mixed surfactant comprising of Span 80 and Tween 80 increased erucic acid production by 57% at a buffer-oil ratio of 0.2:1.

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“…These finding also is supported by [26] which claim that sonication process does not affect the active site of an enzyme due to the different heating concept of ultrasound wave which generate from periodic pressure fluctuation unlike traditional heating. As suggested by [26], overall effect of ultrasonic on enzyme mainly depend on the energy input and irradiation period as these factor will lead to enzyme aggregation, thereby obstructing the active site and decreasing enzyme stability.…”

Section: Activity and Stability Of Enzymes In Ultrasonic Reactionmentioning

confidence: 58%

“…As suggested by [26], overall effect of ultrasonic on enzyme mainly depend on the energy input and irradiation period as these factor will lead to enzyme aggregation, thereby obstructing the active site and decreasing enzyme stability. Thus, in application of the ultrasonic in enzymatic reaction, appropriate wave duty cycles, acoustic power, prolong of exposure and support materials are important in order to retain the stability and activity of the enzyme.…”

Section: Activity and Stability Of Enzymes In Ultrasonic Reactionmentioning

confidence: 99%

Ultrasonic assisted enzymatic reaction: An overview on ultrasonic mechanism and stability-activity of the enzyme

Fazlena

Norsuraya

Nadiah

2013

2013 IEEE Business Engineering and Industrial Applications Colloquium (BEIAC)

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The aim of this study is to review on mechanism of ultrasonic irradiation in enzymatic esterification reaction, the effect on enzyme activity and stability and also rate of reaction in ultrasonic assisted enzymatic reaction. Conventional enzymatic esterification system using immobilized enzyme generally have a limitation in internal mass transfer that might plausible to reduce rate of ester formation. However, this obstacle can be overcome using ultrasonic system that offers different movement of the molecule. The mechanism of ultrasonic is based on the high energy waves that create cavitations in the liquid solution. Better cavitations are attained at high amplitudes, nucleation sites, lower temperature, external pressure and dissolved gas. While for the activity and stability of the enzyme during ultrasonic irradiation process, appropriate wave duty cycles, acoustic power, prolong of exposure and support materials are required in order to retain the stability and activity of the enzyme. Application of ultrasonic irradiation in enzymatic esterification reaction enhanced mass transfer and diffusion of substrate and product within the reaction medium. This factor results in higher reaction rate as compared to the mechanical agitation.

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Successive cycles of utilization of novozym 435 in three different reaction systems

Cited by 31 publications

References 20 publications

Enzymatic synthesis and structural characterization of methacryloyl‐D‐fructose‐ and methacryloyl‐D‐glucose‐based monomers and poly(methacryloyl‐D‐fructose)‐based hydrogels

Enzymatic synthesis and structural characterization of methacryloyl‐D‐fructose‐ and methacryloyl‐D‐glucose‐based monomers and poly(methacryloyl‐D‐fructose)‐based hydrogels

Effects of process variables and additives on mustard oil hydrolysis by porcine pancreas lipase

Ultrasonic assisted enzymatic reaction: An overview on ultrasonic mechanism and stability-activity of the enzyme

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