Successive Solvent Extraction of Polyphenols and Flavonoids from Cistus creticus L. Leaves

Palaiogiannis, Dimitrios; Chatzimitakos, Theodoros; Athanasiadis, Vassilis; Bozinou, Eleni; Makris, Dimitris P.; Lalas, Stavros

doi:10.3390/oxygen3030018

Cited by 12 publications

(5 citation statements)

References 38 publications

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“…However, they require further confirmation through more advanced techniques. The results obtained in our research are worth comparing with those from our previous study [31], given that the same analyses were conducted in both cases, in which solvents of increasing polarity were employed to extract bioactive compounds from C. creticus leaves. The same compounds were determined in the aqueous solutions, but since the extraction conditions were not optimized, the concentrations differed between the two studies.…”

Section: Parametersmentioning

confidence: 53%

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Extraction of Bioactive Compounds from Cistus creticus Leaves and Their Use in the Preparation of Yogurt Desserts

Palaiogiannis,

Athanasiadis,

Chatzimitakos

et al. 2024

Oxygen

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The objective of this study was to determine the optimal conditions for the recovery of bioactive and antioxidant compounds in aqueous solutions of Cistus creticus leaves and then employ the optimal extract for the enrichment of yogurt samples. The optimal conditions were established by a response surface methodology and were determined to be a liquid-to-solid ratio of 48 mL/g at 76 °C for 41 min. The optimum extract yielded TPC 157.17 mg GAE/g dw and TFC 2.38 mg QE/g dw, while FRAP and DPPH values were 1258.52 and 933.67 μmol AAE/g dw, respectively. HPLC‒DAD was utilized to identify and quantify specific polyphenols, like myricetin rhamnoside, in the extract. The optimal extract was then added to yogurt desserts during their preparation at three different concentrations to study how the physicochemical characteristics of the yogurt, as well as the antioxidant capacity added during enrichment, were affected. Statistical analysis of the results was carried out in order to obtain more valid data. It seems that the most suitable concentration for yogurt fortification was 0.1% w/v of the extract as, at this concentration, the yogurts exhibited higher antioxidant capacity, and their physicochemical characteristics were improved.

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Section: Parametersmentioning

confidence: 53%

“…The determination of TPC was carried out using a previously described method [31]. Briefly, 100 µL of C. creticus extracts were combined with 100 µL of Folin-Ciocalteu reagent in a 1.5 mL Eppendorf tube.…”

Section: Quantification Of Total Polyphenol Content (Tpc)mentioning

confidence: 99%

Extraction of Bioactive Compounds from Cistus creticus Leaves and Their Use in the Preparation of Yogurt Desserts

Palaiogiannis,

Athanasiadis,

Chatzimitakos

et al. 2024

Oxygen

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“…The extraction of bioactive compounds from plant material is typically conducted using a single solvent or a solvent mixture. Commonly utilized solvents include water, ethanol, and methanol, as they have proven to be efficient in extracting polyphenols from plants to obtain phenolic compounds that exhibit high solubility in these solvents [ 45 ]. In the present study, ethanol was selected as the solvent for maceration extraction process.…”

Section: Resultsmentioning

confidence: 99%

Anti-Melanogenic Activity of Ethanolic Extract from Garcinia atroviridis Fruits Using In Vitro Experiments, Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation

Tedasen,

Chiabchalard,

Tencomnao

et al. 2024

Antioxidants

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Melanin, the pigment responsible for human skin color, increases susceptibility to UV radiation, leading to excessive melanin production and hyperpigmentation disorders. This study investigated the ethanolic extract of Garcinia atroviridis fruits for its phenolic and flavonoid contents, antioxidant activity, and impact on melanogenesis pathways using qRT-PCR and Western blot analysis. Utilizing network pharmacology, molecular docking, and dynamics simulations, researchers explored G. atroviridis fruit extract’s active compounds, targets, and pharmacological effects on hyperpigmentation. G. atroviridis fruit extract exhibited antioxidant properties, scavenging DPPH• and ABTS•+ radicals radicals and chelating copper. It inhibited cellular tyrosinase activity and melanin content in stimulated B16F10 cells, downregulating TYR, TRP-1, phosphorylated CREB, CREB, and MITF proteins along with transcription levels of MITF, TYR, and TRP-2. LC-MS analysis identified thirty-three metabolites, with seventeen compounds selected for further investigation. Network pharmacology revealed 41 hyperpigmentation-associated genes and identified significant GO terms and KEGG pathways, including cancer-related pathways. Kaempferol-3-O-α-L-rhamnoside exhibited high binding affinity against MAPK3/ERK1, potentially regulating melanogenesis by inhibiting tyrosinase activity. Stable ligand–protein interactions in molecular dynamics simulations supported these findings. Overall, this study suggests that the ethanolic extract of G. atroviridis fruits possesses significant antioxidant, tyrosinase inhibitory, and anti-melanogenic properties mediated through key molecular targets and pathways.

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“…The most effective solvent in the case of thermally modified samples (mainly at a temperature of 180 • C), which extracted the highest percentage of compounds from wood, was ethanol. The high content of ethanol extractives is because it is a more polar solvent than acetone that can dissolve the most represented polyphenols [70]. For that reason, the absorption spectra of the extractives dissolved in ethanol were also analyzed and are presented in Figure 7.…”

Section: In Vitro Color Measurement Of Extractive Compounds Using Uv-...mentioning

confidence: 99%

Chromophores’ Contribution to Color Changes of Thermally Modified Tropical Wood Species

Jurczyková,

Šárovec,

Kačík

et al. 2023

Polymers

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This work examines the effect of thermal modification temperature (180, 200, and 220 °C) in comparison with reference (untreated) samples on selected optical properties of six tropical wood species—Sp. cedar (Cedrala odorata), iroko (Chlorophora excelsa), merbau (Intsia spp.), meranti (Shorea spp.), padouk (Pterocarpus soyauxii), and teak (Tectona grandis). The main goal is to expand the existing knowledge in the field of wood thermal modification by understanding the related degradation mechanisms associated with the formation of chromophoric structures and, above all, to focus on the change in the content of extractive substances. For solid wood, the CIELAB color space parameters (L*, a*, b*, and ΔE*), yellowness (Y), ISO brightness, and UV-Vis diffuse reflectance spectra were obtained. Subsequently, these wood samples were extracted into three individual solvents (acetone, ethanol, and ethanol-toluene). The yields of the extracted compounds, their absorption spectra, and again L*, a*, b*, ΔE*, and Yi parameters were determined. With increasing temperatures, the samples lose brightness and darken, while their total color difference grows (except merbau). The highest yield of extractives (mainly phenolic compounds, glycosides, and dyes) from thermally modified samples was usually obtained using ethanol. New types of extractives (e.g., 2-furaldehyde, lactones, formic acid, some monomer derivatives of phenols, etc.) are already created around a temperature of 180 °C and may undergo condensation reactions at higher temperatures. For padouk, merbau, teak, and partially iroko modified at temperatures of 200 and 220 °C, there was a detected similarity in the intensities of their UV-Vis DR spectra at the wavelength regions corresponding to phenolic aldehydes, unsaturated ketones, quinones, stilbenes, and other conjugated carbonyl structures. Overall, a statistical assessment using PCA sorted the samples into five clusters. Cluster 3 consists of almost all samples modified at 200 and 220 °C, and in the other four, the reference and thermally modified samples at 180 °C were distributed. The yellowness of wood (Y) has a very high dependence (r = 0.972) on its brightness (L*) and the yellowness index of the extractives in acetone Yi(Ac), whose relationship was described by the equation Y = −0.0951 × Y(Ac) + 23.3485.

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Successive Solvent Extraction of Polyphenols and Flavonoids from Cistus creticus L. Leaves

Cited by 12 publications

References 38 publications

Extraction of Bioactive Compounds from Cistus creticus Leaves and Their Use in the Preparation of Yogurt Desserts

Extraction of Bioactive Compounds from Cistus creticus Leaves and Their Use in the Preparation of Yogurt Desserts

Anti-Melanogenic Activity of Ethanolic Extract from Garcinia atroviridis Fruits Using In Vitro Experiments, Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation

Chromophores’ Contribution to Color Changes of Thermally Modified Tropical Wood Species

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