Succinic acid production from duckweed (Landoltia punctata) hydrolysate by batch fermentation of Actinobacillus succinogenes GXAS137

Shen, Naikun; Wang, Qingyan; Zhu, Jing; Qin, Yan; Liao, Saihu; Yi, Li; Zhu, Qixia; Jin, Yanling; Du, Liqin; Huang, Ribo

doi:10.1016/j.biortech.2016.03.036

Cited by 45 publications

(22 citation statements)

References 24 publications

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“…The succinic acid concentration and yield obtained in our work based on the batch fermentation in CSTR using CG as the carbon source are in the same range to those reported in previous works (Table 3). A. succinogenes was studied for succinic acid production from different renewable carbon sources, for examples, glucose, oil palm trunk sap, carob pods, bagasse, duckweed and fresh cassava roots which resulted in the final succinic acid concentration and product yield of 9.4-151.4 g/L and 0.4-1.5 g/g., respectively [10][11][12][13][14][15][16][17][18]. The product titer and yield obtained in our study were also comparable to those previously reported using A. succinogenes with glycerol as the substrate in batch fermentation which was 12.8 g/L and 0.9 g/g [18].…”

Section: Effect Of Dissolved Co 2 From Initial Co 2 Purging On Batch supporting

confidence: 84%

“…Production of bio-succinic acid from diverse substrates has been reported using different microorganisms and mode of operations in fermentation. These microbes include Mannheimia succinoproducens, Yarrowia lipolytica and Actinobacillus succinogenes which resulted in the final succinic acid titer in the range of 9.4-151.4 g/L with the product yield ranging from 0.4-1.5 g/g [10][11][12][13][14][15][16][17][18]. Among various microbes, A. succinogenes is a potent succinic acid producer with advantages on its capability to produce high concentrations of succinic acid and high tolerance to acid produced during fermentation [19] However, studies on utilization of CG by A. succinogenes for succinic acid production have been limited to only very few reports [19] while its relevant metabolic pathways are not well characterized.…”

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confidence: 99%

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Optimization of bio-succinic fermentation process from crude glycerol by Actinobacillus succinogenes

Kanchanasuta

Champreda

Pisutpaisal

et al. 2020

Environmental Engineering Research

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Bio-succinic acid is a commodity chemical with potent application in bioplastic and food industries which can be produced from renewable resources. In this study, bioprocess for production of bio-succinic from glycerol by Actinobacillus succinogenes was studied. The maximum succinic acid concentration in small-scale serum bottle experiments was 6.8 and 6.5 g/L using pure and crude glycerol, respectively, with supplemented MgCO3. The ratio of acetic acid to succinic acid (AA/SA) implied the increased carbon flux to the C4 pathway using crude glycerol supplemented with MgCO3 compared to that with CaCO3.The carbonate salts tended to induce C3 metabolic pathway in fermentation using pure glycerol which was in accordance with the ratio of acetic acid to glycerol (AA/GL). The highest succinic acid concentration of 17.9 g/L from crude glycerol was achieved from batch fermentation in a lab-scale fermenter with the maximum glycerol utilization of 99.9% which were higher than those obtained from fed- batch and semi-continuous processes. Acetic acid tended to increase throughout the fermentation process in fed-batch and semi-continuous operations, which resulted in the lower product yield and substrate utilization efficiency. The time for initial purging of CO2 showed effects on succinic acid production and internal metabolic pathways. This work provided a basis for process development on bio-succinic acid production from crude glycerol in industry.

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Section: Effect Of Dissolved Co 2 From Initial Co 2 Purging On Batch supporting

confidence: 84%

mentioning

confidence: 99%

Optimization of bio-succinic fermentation process from crude glycerol by Actinobacillus succinogenes

Kanchanasuta

Champreda

Pisutpaisal

et al. 2020

Environmental Engineering Research

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“…Bacterial culture was then used to inoculate fermentation medium (5% (v/v)) with the following composition (g/L): carbon source (100); yeast extract (5); peptone (5); urea (2); K 2 HPO 4 (1), MgSO 4 × 7H 2 O (0,2); CaCl 2 (0,5); MgCO 3 (60). MgCO 3 was added as a pH buffer of the fermentation broth 64 . Carbon (C) and nitrogen (N) sources were sterilized separately for 20 min at 121 °C before use and then were mixed together aseptically.…”

Section: Methodsmentioning

confidence: 99%

Genome analysis of a wild rumen bacterium Enterobacter aerogenes LU2 - a novel bio-based succinic acid producer

Szczerba

Komoń-Janczara

Krawczyk

et al. 2020

Sci Rep

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Enterobacter aerogenes LU2 was isolated from cow rumen and recognized as a potential succinic acid producer in our previous study. Here, we present the first complete genome sequence of this new, wild strain and report its basic genetic features from a biotechnological perspective. The MinION singlemolecule nanopore sequencer supported by the Illumina MiSeq platform yielded a circular 5,062,651 bp chromosome with a GC content of 55% that lacked plasmids. A total of 4,986 genes, including 4,741 protein-coding genes, 22 rRNA-, 86 tRNA-, and 10 ncRNA-encoding genes and 127 pseudogenes, were predicted. The genome features of the studied strain and other Enterobacteriaceae strains were compared. Functional studies on the genome content, metabolic pathways, growth, and carbon transport and utilization were performed. The genomic analysis indicates that succinic acid can be produced by the LU2 strain through the reductive branch of the tricarboxylic acid cycle (TCA) and the glyoxylate pathway. Antibiotic resistance genes were determined, and the potential for bacteriocin production was verified. Furthermore, one intact prophage region of length ~31,9 kb, 47 genomic islands (GIs) and many insertion sequences (ISs) as well as tandem repeats (TRs) were identified. No clustered regularly interspaced short palindromic repeats (CRISPRs) were found. Finally, comparative genome analysis with well-known succinic acid producers was conducted. The genome sequence illustrates that the LU2 strain has several desirable traits, which confirm its potential to be a highly efficient platform for the production of bulk chemicals. Enterobacter aerogenes LU2 is a gram-negative, wild bacterium that was isolated from cow rumen as a part of environmental screening for succinic acid-producing bacteria identification. Succinic acid (SA) plays an important role as a metabolic intermediate in the rumen by increasing propionate production, a crucial energy source for the ruminant 1,2. Anaerobic conditions and the presence of carbon dioxide, methane and trace amounts of hydrogen create an excellent environment for the biosynthesis of succinate by some bacteria existing in the rumen 3. In reports of the U.S. Department of Energy (DOE) from 2004 and 2010, SA was recognized as one of the top 10 most promising C4-building chemical platforms for the production of high-value commodity and specialty chemicals with great industrial potential 2,4. Succinate is widely applied as an additive in food, pharmaceuticals, detergents, solvents, and surfactants as well as in biodegradable polymer production 5,6. Until recently, industrial production of succinate was based on chemical synthesis. However, petroleum-based production of SA from n-butane through maleic anhydrate requires the use of high pressure, high temperature and costly catalysts 7. Therefore, because of the current global trend regarding sustainable development, including the support of green technologies, rational waste biomass management and pollution-reducing standards, the bio-based production of...

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“…Until recently, succinate was commercially produced from n-butane through maleic anhydrate by a chemical process requiring the use of costly catalysts, and this process contributed to environmental problems [7,8]. Therefore, because of pollution-reducing standards and global trends towards rational waste biomass management, the establishment of sustainable processes for the microbial production of SA from renewable feedstock has become a focal point of global interest [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

A novel biocatalyst, Enterobacter aerogenes LU2, for efficient production of succinic acid using whey permeate as a cost-effective carbon source

Szczerba

Komoń-Janczara

Dudziak

et al. 2020

Biotechnol Biofuels

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Background: Succinic acid (SA), a valuable chemical compound with a broad range of industrial uses, has become a subject of global interest in recent years. The bio-based production of SA by highly efficient microbial producers from renewable feedstock is significantly important, regarding the current trend of sustainable development. Results: In this study, a novel bacterial strain, LU2, was isolated from cow rumen and recognized as an efficient producer of SA from lactose. Proteomic and genetic identifications as well as phylogenetic analysis were performed, and strain LU2 was classified as an Enterobacter aerogenes species. The optimal conditions for SA production were 100 g/L lactose, 10 g/L yeast extract, and 20% inoculum at pH 7.0 and 34 °C. Under these conditions, approximately 51.35 g/L SA with a yield of 53% was produced when batch fermentation was conducted in a 3-L stirred bioreactor. When lactose was replaced with whey permeate, the highest SA concentration of 57.7 g/L was achieved with a yield and total productivity of 62% and 0.34 g/(L*h), respectively. The highest productivity of 0.67 g/(L*h) was observed from 48 to 72 h of batch fermentation, when E. aerogenes LU2 produced 16.23 g/L SA. Conclusions: This study shows that the newly isolated strain E. aerogenes LU2 has great potential as a new biocatalyst for producing SA from whey permeate.

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Succinic acid production from duckweed (Landoltia punctata) hydrolysate by batch fermentation of Actinobacillus succinogenes GXAS137

Cited by 45 publications

References 24 publications

Optimization of bio-succinic fermentation process from crude glycerol by Actinobacillus succinogenes

Optimization of bio-succinic fermentation process from crude glycerol by Actinobacillus succinogenes

Genome analysis of a wild rumen bacterium Enterobacter aerogenes LU2 - a novel bio-based succinic acid producer

A novel biocatalyst, Enterobacter aerogenes LU2, for efficient production of succinic acid using whey permeate as a cost-effective carbon source

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