Succinyl‐CoA Synthetase Structure‐Function Relationships and Other Considerations

Nishimura, Jonathan S.

doi:10.1002/9780470123041.ch4

Cited by 22 publications

(17 citation statements)

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“…N -3 PUFA was previously found to regulate the metabolic function of liver effectively by increasing MDH enzyme activity of rat liver [59,72]. SCS catalyzes the reversible reaction of succinyl-CoA to succinate, and is the only mitochondrial enzyme capable of ATP production via substrate level phosphorylation in the absence of oxygen [73]. SCS plays a key role in the citric acid cycle, ketone metabolism and heme synthesis [74].…”

Section: Resultsmentioning

confidence: 99%

Novel regulatory roles of omega-3 fatty acids in metabolic pathways: a proteomics approach

et al. 2014

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BackgroundOmega-3 polyunsaturated fatty acids (n-3 PUFA) have been shown to alleviate the symptoms of metabolic disorders, such as heart disease, diabetes, obesity and insulin resistance. Several putative mechanisms by which n-3 PUFA elicit beneficial health effects have been proposed; however, there is still a shortage of knowledge on the proteins and pathways that are regulated by n-3 PUFA.MethodsUsing two dimensional polyacrylamide gel electrophoresis (2D-PAGE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, we investigated the effects of diets high or low in n-3 PUFA on hepatic proteomic profile of C57BL/6 mice.ResultsThe findings show for the first time that high dietary n-3 PUFA reduced the expression of regucalcin, adenosine kinase and aldehyde dehydrogenase. On the other hand, diets high in n-3 PUFA increased the expression of apolipoprotein A-I, S-adenosylmethionine synthase, fructose-1, 6-bisphosphatase, ketohexokinase, malate dehydrogenase, GTP-specific succinyl CoA synthase, ornithine aminotransferase and protein disulfide isomerase-A3.ConclusionsOur findings revealed for the first time that n-3 PUFA causes alterations in several novel functional proteins involved in regulating lipid, carbohydrate, one-carbon, citric acid cycle and protein metabolism, suggesting integrated regulation of metabolic pathways. These novel proteins are potential targets to develop therapeutic strategies against metabolic disorders.

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Section: Resultsmentioning

confidence: 99%

Novel regulatory roles of omega-3 fatty acids in metabolic pathways: a proteomics approach

et al. 2014

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“…The molecular mass of the ␣ and ␤ subunits are ϳ30 and ϳ40 kDa, respectively (19), and there are fundamental differences in the quaternary structures of the bacterial and mammalian forms of succinyl-CoA synthetases. The Escherichia coli enzyme functions as an ␣ 2 ␤ 2 tetramer with no activity associated with a heterodimeric form, whereas the enzyme isolated from pig heart functions only as the ␣␤ dimer (20).…”

Section: Dementioning

confidence: 99%

Proteomics Analysis of Thermoplasma acidophilum with a Focus on Protein Complexes

Sun

Beck

Knispel

et al. 2007

Molecular & Cellular Proteomics

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Two-dimensional gel electrophoresis (2DE) and MALDI-TOF MS were used to obtain a global view of the cytoplasmic proteins expressed by Thermoplasma acidophilum. In addition, glycerol gradient ultracentrifugation coupled to 2DE-MALDI-TOF MS analysis was used to identify subunits of macromolecular complexes. With the 2DE proteomics approach, over 900 spots were resolved of which 271 proteins were identified. A significant number of these form macromolecular complexes, among them the ribosome, proteasome, and thermosome, which are expressed at high levels. In the glycerol gradient heavy fractions, 10 as yet uncharacterized proteins (besides the well known ribosomal subunits, translation initiation factor eIF-6-related protein, elongation factor 1, and DNA-dependent RNA polymerase) were identified that are putative building blocks of protein complexes. These proteins belong to the categories of hypothetical or conserved hypothetical proteins, and they are present in the cytosol at low concentrations. Although these proteins exhibit homology to known sequences, their structures, subunit compositions, and biological functions are not yet known. Molecular & Cellular Proteomics 6: 492-502, 2007.

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“…Succinyl-CoA synthetases (SucCD; EC 6.2.1.4 or EC 6.2.1.5) occur in prokaryotes and eukaryotes and are widely known for catalyzing the only substrate-level phosphorylation in the citric acid cycle (7,31). Therein, the conversion of succinyl-CoA to succinate yields nucleoside triphosphates during aerobic metabolism.…”

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confidence: 99%

Novel Reaction of Succinyl Coenzyme A (Succinyl-CoA) Synthetase: Activation of 3-Sulfinopropionate to 3-Sulfinopropionyl-CoA in Advenella mimigardefordensis Strain DPN7 ^T during Degradation of 3,3′-Dithiodipropionic Acid

et al. 2011

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The sucCD gene of Advenella mimigardefordensis strain DPN7 T encodes a succinyl coenzyme A (succinyl-CoA) synthetase homologue (EC 6.2.1.4 or EC 6.2.1.5) that recognizes, in addition to succinate, the structural analogues 3-sulfinopropionate (3SP) and itaconate as substrates. Accumulation of 3SP during 3,3-dithiodipropionic acid (DTDP) degradation was observed in Tn5::mob-induced mutants of A. mimigardefordensis strain DPN7T disrupted in sucCD and in the defined deletion mutant A. mimigardefordensis ⌬sucCD. These mutants were impaired in growth with DTDP and 3SP as the sole carbon source. Hence, it was proposed that the succinyl-CoA synthetase homologue in A. mimigardefordensis strain DPN7T activates 3SP to the corresponding CoA-thioester (3SP-CoA). The putative genes coding for A. mimigardefordensis succinyl-CoA synthetase (SucCD Am ) were cloned and heterologously expressed in Escherichia coli BL21(DE3)/pLysS. Purification and characterization of the enzyme confirmed its involvement during degradation of DTDP. 3SP, the cleavage product of DTDP, was converted into 3SP-CoA by the purified enzyme, as demonstrated by in vitro enzyme assays. The structure of 3SP-CoA was verified by using liquid chromatography-electrospray ionization-mass spectrometry. SucCD Am is Mg 2؉ or Mn 2؉ dependent and unspecific regarding ATP or GTP. In kinetic studies the enzyme showed highest enzyme activity and substrate affinity with succinate (V max ‫؍‬ 9.85 ؎ 0.14 mol min ؊1 mg ؊1 , K m ‫؍‬ 0.143 ؎ 0.001 mM). In comparison to succinate, activity with 3SP was only ca. 1.2% (V max ‫؍‬ 0.12 ؎ 0.01 mol min ؊1 mg ؊1 ) and the affinity was 6-fold lower (K m ‫؍‬ 0.818 ؎ 0.046 mM). Based on the present results, we conclude that SucCD Am is physiologically associated with the citric acid cycle but is mandatory for the catabolic pathway of DTDP and its degradation intermediate 3SP.3,3Ј-Dithiodipropionic acid (DTDP) is an organic disulfide and a precursor for the production of polythioesters (PTEs) by bacteria (25). Further applications for DTDP are thermodynamic studies (40), development of secondary batteries (52), amino acid analysis (53), and the construction of self-assembling monolayers (10). Microbial production of PTEs from simple carbon sources and inorganic sulfur is currently not possible. Knowledge of the catabolism of organic sulfur compounds in bacteria could provide a reasonable strategy to engineer strains suitable for PTE production. A first step in this direction was the isolation of bacteria able to utilize DTDP as the sole source of carbon and energy. Advenella mimigardefordensis strain DPN7T , a betaproteobacterium, found in mature compost in a waste management facility was one of the isolates (15,56

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Succinyl‐CoA Synthetase Structure‐Function Relationships and Other Considerations

Cited by 22 publications

References 96 publications

Novel regulatory roles of omega-3 fatty acids in metabolic pathways: a proteomics approach

Novel regulatory roles of omega-3 fatty acids in metabolic pathways: a proteomics approach

Proteomics Analysis of Thermoplasma acidophilum with a Focus on Protein Complexes

Novel Reaction of Succinyl Coenzyme A (Succinyl-CoA) Synthetase: Activation of 3-Sulfinopropionate to 3-Sulfinopropionyl-CoA in Advenella mimigardefordensis Strain DPN7 ^T during Degradation of 3,3′-Dithiodipropionic Acid

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Succinyl‐CoA Synthetase Structure‐Function Relationships and Other Considerations

Cited by 22 publications

References 96 publications

Novel regulatory roles of omega-3 fatty acids in metabolic pathways: a proteomics approach

Novel regulatory roles of omega-3 fatty acids in metabolic pathways: a proteomics approach

Proteomics Analysis of Thermoplasma acidophilum with a Focus on Protein Complexes

Novel Reaction of Succinyl Coenzyme A (Succinyl-CoA) Synthetase: Activation of 3-Sulfinopropionate to 3-Sulfinopropionyl-CoA in Advenella mimigardefordensis Strain DPN7 T during Degradation of 3,3′-Dithiodipropionic Acid

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Novel Reaction of Succinyl Coenzyme A (Succinyl-CoA) Synthetase: Activation of 3-Sulfinopropionate to 3-Sulfinopropionyl-CoA in Advenella mimigardefordensis Strain DPN7 ^T during Degradation of 3,3′-Dithiodipropionic Acid