Sucrose

Bergmeyer, Hans‐Ulrich; Klotzsch, Helmut

doi:10.1016/b978-0-12-395630-9.50019-0

Cited by 42 publications

(22 citation statements)

References 3 publications

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“…Other analyses. Glucose was analysed by an enzymic method using hexokinase and glucose-6-phosphate dehydrogenase (Bergmeyer & Klotsch, 1965). Protein from NaOH-hydrolysed cells (0.2 M NaOH, 100 "C, 15 min) was assayed by the Lowry method.…”

Section: Introductionmentioning

confidence: 99%

The ability of Escherichia coli O157:H7 to decrease its intracellular pH and resist the toxicity of acetic acid

1997

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Batch cultures of Escherichia coli K-12 grew well in an anaerobic glucose medium a t pH 5.9, but even small amounts of acetate (20 mM) inhibited growth and fermentation. E. coli 0157: H7 was a t least fourfold more resistant to acetate than K-12. Continuous cultures of E. coli K-12 (pH 5.9, dilution rate 0085 h'l) did not wash out until the sodium acetate concentration in the input medium was 80 mM, whereas E. coli 01 57 : H7 persisted until the sodium acetate concentration was 160 mM. E. coli K-12 cells accumulated as much as 500 mM acetate, but the intracellular acetate concentration of 01 57 : H7 was never greater than 300 mM. Differences in acetate accumulation could be explained by intracellular pH and the transmembrane pH gradient (ApH). E. coli K-12 maintained a more or less constant ApH (intracellular pH 68), but E. coli 0157:H7 let its ApH decrease from 0.9 to 0.2 units as sodium acetate was added to the medium. Sodium acetate increased the rate of glucose consumption, but there was little evidence to support the idea that acetate was creating a futile cycle of protons. Increases in glucose consumption rate could be explained by increases in D-lactate production and decreases in ATP production. lntracellular acetate was initially lower than the amount predicted by ApH, but intracellular acetate and ApH were in equilibrium when the external acetate concentrations were high. Based on these results, the acetate tolerance of 0157: H7 can be explained by fundamental differences in metabolism and intracellular pH regulation. By decreasing the intracellular pH and producing large amounts of D-lactate, 0157:H7 is able to decrease ApH and prevent toxic accumulations of intracellular acetate anion.

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Section: Introductionmentioning

confidence: 99%

The ability of Escherichia coli O157:H7 to decrease its intracellular pH and resist the toxicity of acetic acid

1997

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“…The supernatant was used to analyze soluble sugars. Glucose, fructose and sucrose in the supernatant were determined by coupled enzymatic assay (Bergmeyer and Bernt, 1974).…”

Section: Analysis Of Sugars and Starchmentioning

confidence: 99%

“…The washed pellet was suspended in distilled water, and the suspension was heated at 90ºC for 2 hr. Gelatinized starch was digested with amyloglucosidase at 55ºC for 30 min and resultant glucose determined based on the method mentioned above (Bergmeyer and Bernt, 1974). Starch content was expressed as the amount of glucose per unit fresh weight of shoot or root.…”

Section: Analysis Of Sugars and Starchmentioning

confidence: 99%

Seedling Growth and Dry-Matter Production under Drained Conditions in Rice Direct-Sown into Puddled and Leveled Soil

Satô

Maruyama

2005

Plant Production Science

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“…Determination of carbohydrate and chlorophyll contents and canopy net-photosynthesis Samples of approximately 50 mg dry weight were powdered in liquid nitrogen with a mortar and pestle and extracted twice with 80% (v/v) ethanol at 80°C. After centrifugation at 2000Xg for 5 min, the supernatants were collected, dried under vacuum, and used for the enzymatic determination of sucrose and glucose contents (Bergmeyer and Bernt, 1974). For the determination of the starch content, the pellets from centrifugation were boiled in distilled water for 2 h and digested with amyloglucosidase for 15 min at 55°C.…”

Section: Growth Conditionsmentioning

confidence: 99%

Effects of Elevated CO₂Concentration on Photosynthetic Carbon Metabolism in Flag-Leaf Blades of Rice before and after Heading

Aoki

Ono

Sasaki

et al. 2003

Plant Production Science

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Sucrose

Cited by 42 publications

References 3 publications

The ability of Escherichia coli O157:H7 to decrease its intracellular pH and resist the toxicity of acetic acid

The ability of Escherichia coli O157:H7 to decrease its intracellular pH and resist the toxicity of acetic acid

Seedling Growth and Dry-Matter Production under Drained Conditions in Rice Direct-Sown into Puddled and Leveled Soil

Effects of Elevated CO₂Concentration on Photosynthetic Carbon Metabolism in Flag-Leaf Blades of Rice before and after Heading

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Sucrose

Cited by 42 publications

References 3 publications

The ability of Escherichia coli O157:H7 to decrease its intracellular pH and resist the toxicity of acetic acid

The ability of Escherichia coli O157:H7 to decrease its intracellular pH and resist the toxicity of acetic acid

Seedling Growth and Dry-Matter Production under Drained Conditions in Rice Direct-Sown into Puddled and Leveled Soil

Effects of Elevated CO2Concentration on Photosynthetic Carbon Metabolism in Flag-Leaf Blades of Rice before and after Heading

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Effects of Elevated CO₂Concentration on Photosynthetic Carbon Metabolism in Flag-Leaf Blades of Rice before and after Heading