Sucrose Density Gradient Analysis of 1,25-Dihydroxyvitamin D<sub>3</sub>Binding in Human Breast Tumors*

Christakos, Sylvia; Sori, Alan J.; Greenstein, Stuart; Murphy, Thomas F.

doi:10.1210/jcem-56-4-686

Cited by 6 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The milk, although altered in composition, appears to be nutritionally adequate (41), but the full nutritional needs of the rachitic pups are apparently not always met (42)(43)(44). The presence of specific receptors for vitamin D in normal and neoplastic breast tissues (11)(12)(13)(14)(15)(16)(17) has prompted investigations by several laboratories into a direct role of vitamin D on mammary gland function. Human breast tumor cells in long term tissue culture have been shown to respond to the addition of vitamin FlG.…”

Section: Discussionmentioning

confidence: 99%

“…Binding specificity for these receptors parallels known biological potencies of vitamin D analogs (15). The cytoplasmic receptor proteins are highly analogous to those of steroid hormones (17,20) and are known to translocate to the nucleus (20). Thawmount autoradiographic analysis of binding to normal rat mammary gland tissue has clearly demonstrated that the specific nuclear concentration of 1,25-(OH) 2 D 3 is in the epithelial cells of mammary alveoli and cells of the excretory ducts, but not connective tissue cells and myoepithelial cells (11).…”

mentioning

confidence: 98%

“…Normal mammary glands from rats (11) and mice (12) as well as human breast tumors (13) and established cell lines (14)(15)(16)(17) have specific receptors for the active vitamin D metabolite 1,25-dihydroxycholecalciferol [1,25-(OH) 2 D 3 ]. The receptors found in the breast tissue are very similar in physical characteristics and binding properties to those shown in other target tissues, such as intestinal mucosa (18,19).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Milk Protein Synthesis by Mammary Glands of Vitamin D-Deficient Mice

1987

View full text Add to dashboard Cite

Mammary glands from second generation vitamin D-deficient mice and rats were examined for their ability to make the major milk proteins, casein and a-lactalbumin, both in vivo and in vitro. The glands from the rachitic animals were morphologically indistinguishable from those of age-matched controls. When placed in explant culture, glands from vitamin D-deficient mice and rats underwent DNA synthesis at a rate comparable to that of glands from the vitamin D-replete controls. However, the hormonally induced synthesis of casein and a-lactalbumin was significantly reduced in explants of glands from rachitic us. control animals. The reduction in caseinsynthesizing ability by mouse mammary gland explants was not reversed by the addition of 10~8 or 10~6 M 1,25-dihydroxycholecalciferol to the culture medium, but could be reversed by pretreating the vitamin D-deficient mice with the D metabolite in vivo for 10 days before the onset of culture. The decrease in milk protein synthesis in culture is paralleled in vivo by a decrease in milk protein content in the milk and lactating mammary glands of vitamin D-deficient mice and rats. Both in vivo and in vitro, it is the two highest mol wt caseins that are most affected by the lack of vitamin D in the diet. These data suggest that vitamin D does not play a fundamental role in growth and morphological development of the normal mammary gland, but, rather, it is important in maintenance of full hormonally induced functional differentiation of the mature gland. (Endocrinology 121:865-874,1987)

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

See 1 more Smart Citation

Milk Protein Synthesis by Mammary Glands of Vitamin D-Deficient Mice

1987

View full text Add to dashboard Cite

show abstract

1α-hydroxyvitamin D3, hypercalcemia, and growth suppression of 7,12-dimethylbenz[a]anthracene-induced rat mammary tumors

Iino

Yoshida

Sugamata

et al. 1992

Breast Cancer Res Tr

View full text Add to dashboard Cite

1 alpha-hydroxyvitamin D3 [1 alpha(OH)D3] was administered to female Sprague-Dawley rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary tumors. 1 alpha(OH)D3 suppressed the growth of the rat mammary tumors dose-dependently, and in the high dose groups treated with 0.5-1.0 micrograms/kg of 1 alpha(OH)D3, significant inhibition of tumor growth was observed. But daily oral administration of 1 alpha(OH)D3 for four consecutive weeks caused side effects such as hypercalcemia and weight loss. We compared 0.5 microgram/kg of 1 alpha(OH)D3 three times weekly with the same dose six times weekly to discover whether or not the side effects can be reduced by treatment schedule. Both groups showed a significant oncostatic effect, compared with the control group, while the side effects were relieved in the three times weekly group. Regarding estrogen receptors (ER) in the tumors, there was no significant difference among the groups. These results suggested that the antitumor effect of 1 alpha(OH)D3 on DMBA-induced mammary tumors was not related to ER status. Combined use of 1 alpha(OH)D3 with 5-fluorouracil (5-FU) or medroxyprogesterone acetate (MPA) was also examined. No significant augmentation of the antitumor effect was seen in the two combinations, although the combined therapy with MPA showed a significant inhibition of weight loss in the rats.

show abstract

Vitamin D and Breast Cancer

et al. 1989

View full text Add to dashboard Cite

Sucrose Density Gradient Analysis of 1,25-Dihydroxyvitamin D₃Binding in Human Breast Tumors*

Cited by 6 publications

References 0 publications

Milk Protein Synthesis by Mammary Glands of Vitamin D-Deficient Mice

Milk Protein Synthesis by Mammary Glands of Vitamin D-Deficient Mice

1α-hydroxyvitamin D3, hypercalcemia, and growth suppression of 7,12-dimethylbenz[a]anthracene-induced rat mammary tumors

Vitamin D and Breast Cancer

Contact Info

Product

Resources

About

Sucrose Density Gradient Analysis of 1,25-Dihydroxyvitamin D3Binding in Human Breast Tumors*

Cited by 6 publications

References 0 publications

Milk Protein Synthesis by Mammary Glands of Vitamin D-Deficient Mice

Milk Protein Synthesis by Mammary Glands of Vitamin D-Deficient Mice

1α-hydroxyvitamin D3, hypercalcemia, and growth suppression of 7,12-dimethylbenz[a]anthracene-induced rat mammary tumors

Vitamin D and Breast Cancer

Contact Info

Product

Resources

About

Sucrose Density Gradient Analysis of 1,25-Dihydroxyvitamin D₃Binding in Human Breast Tumors*