Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum

Todorović, Slađana; Grubišić, Dragoljub; Giba, Zlatko; Mišić, Danijela; Konjević, Radomir

doi:10.1007/s10535-006-0128-8

Cited by 11 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among that, 2% showed maximum flower production (22.8) and good response (92.73%). The results of this study are in agreement with earlier reports [22] , [23] .…”

Section: Discussionsupporting

confidence: 94%

In vitro flowering in Oldenlandia umbellata L.

Behera

Chandrasekaran

Payas

et al. 2018

Journal of Ayurveda and Integrative Medicine

View full text Add to dashboard Cite

BackgroundOldenlandia umbellata L. (Indian madder) is an antique Ayurvedic Indian herb and a source of various anthraquinone derivatives. The red dye from its roots has been used in diverse applications since ancient times.ObjectivesTo establish reliable and effective protocols for in vitro flowering of O. umbellata.Materials and methodsFor in vitro flowering, organogenic calli were subcultured onto Murashige and Skoog (MS) medium supplemented with various concentrations of Naphthalene acetic acid (NAA) (0.15–1.0 mg/l) and Benzyladenine(BA) (0.5–1.5 mg/l) with and without 0.4% of coconut milk (CM).ResultsThe highest number of in vitro flowers (22.8%) and best response (92.73%) was achieved on MS medium supplemented with 0.7 mg/l NAA + 1.5 mg/l BA with 0.4% CM. It was found that MS medium devoid of BA promoted best root development (47.3 per calli) as well as response (100%). It was also observed that when embryogenic calli grown in depletion of required nutrition transferred to fresh media induced more flowering. In vivo and in vitro floral comparative analysis revealed that in vitro flower induction was required for short time duration (20.67 days) than in vivo flower.ConclusionTo the best of our knowledge, this is the first report on in vitro flowering and this study will help to overcome problems associated with flower development and seed production. As a result, this study may be a potent conservation tool to restore innate population size in its natural habitat.

show abstract

“…Among that, 2% showed maximum flower production (22.8) and good response (92.73%). The results of this study are in agreement with earlier reports [22] , [23] .…”

Section: Discussionsupporting

confidence: 94%

In vitro flowering in Oldenlandia umbellata L.

Behera

Chandrasekaran

Payas

et al. 2018

Journal of Ayurveda and Integrative Medicine

View full text Add to dashboard Cite

show abstract

“…Seed washing preparation, sowing method, and in vitro germination culture were conducted using some modifications of the protocols previously reported [12][13][14] . Dandelion seeds (Taraxacum platycarpum H. Dablstaed) were washed, and only viable seeds, determined by floatation method, were used.…”

Section: Plant Seed Materialsmentioning

confidence: 99%

Optimization of in vitro seed germination of Taraxacum platycarpum

Lee¹,

Kim²,

Oh³

et al. 2009

Korean Journal of Environmental Agriculture

View full text Add to dashboard Cite

Dandelion (Taraxacum platycarpum) has been widely utilized for medicinal purposes. However, the dandelion seeds are relatively difficult to germinate under cultivation conditions, which hampers seedling propagation of dandelion plants and reduces the opportunity of usage of such a useful medicinal plant. Thus, in this study, in vitro conditions for the dandelion seed germination were optimized to enhance the germination rate. In seed washing steps, the sequential treatments with 20% of ethanol, 20% of NaOCl, and distilled water avoided microbial contamination with the highest in vitro germination rate (67.5%) from seeds sown in germination media. The media supplemented with 1.4 g/L of MS salts and 1% of sucrose significantly enhanced the germination rate compared to the media with 4.4 g/L of MS and 3% of sucrose. Sowing the seeds vertically in the optimized media supplement conditions, 1.4 g/L of MS salts and 1% of sucrose, gave the maximum in vitro germination rate (61%), which was almost three times higher than sowing seeds on a soil pot (23%). Our results indicate that the seed washing and sowing methods including germination medium supplements can be optimized to enhance in vitro seed germination of dandelion.

show abstract

“…Beside different concentrations of phytohormones that affect the development of in vitro cultures, important components are carbohydrates as sources of energy and carbon and osmotic agents that regulate the uptake of other culture constituents. In addition, the concentration and type of a carbohydrate in a medium can affect organogenesis and the development of an in vitro culture (Mohamed and Alsadon, 2010;Nowak et al, 2004;Todorović et al, 2006;Thorpe et al, 2008). Although sucrose is the most widely used carbohydrate and the cheapest one (Thorpe et al, 2008), other carbohydrates, mainly glucose and fructose, had a better effect on the development of in vitro cultures of some species, i.e.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of the Endangered and Decorative Specie <i>Dianthus serotinus</i> Waldst. et Kit.

Marković

Grbić

Djukić

2013

Not Bot Hort Agrobot Cluj

View full text Add to dashboard Cite

During past decades, great attention has been paid to propagation of endangered plant taxa in order to preserve biodiversity. The aim of this study was to optimize a protocol for in vitro propagation of the critically endangered and decorative species Dianthus serotinus Waldst. et Kit. The effects of different concentration of MS salt (Murashige and Skoog) of the culture, medium pH and different carbohydrates (sucrose, glucose, and fructose) on shoot multiplication were examined. The best results were obtained on half-strength MS (Murashige and Skoog) medium, whose pH was 5.8, with sucrose supplied at a concentration of 3%, when shoots with 1-2 nodes or shoot tips (with terminal buds only) were used as explants. The shoots were rooted (76.7%) on half-strength MS medium containing 0.5 mg∙L-1 NAA (1-naphthaleneacetic acid). The obtained plantlets were successfully acclimatized (89%) in a 4:1 mixture of peat and sand and they flowered the following year. Presented protocol enables successful in vitro propagation of D. serotinus.

show abstract

Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum

Cited by 11 publications

References 22 publications

In vitro flowering in Oldenlandia umbellata L.

In vitro flowering in Oldenlandia umbellata L.

Optimization of in vitro seed germination of Taraxacum platycarpum

Micropropagation of the Endangered and Decorative Specie <i>Dianthus serotinus</i> Waldst. et Kit.

Contact Info

Product

Resources

About