Sucrose Matters. The Need to Make Groups Truly Comparable When Assessing Changes Associated with Insulin Sensitivity. Comment on “Consumption of Cooked Black Beans Stimulates a Cluster of Some Clostridia Class Bacteria Decreasing Inflammatory Response and Improving Insulin Sensitivity.” Nutrients 2020, 12(4), 1182

Abstract

The recent paper by Sanchez-Tapia et al [...]

Dietary pulses as a means to improve the gut microbiome, inflammation, and appetite control in obesity

“…This study design, particularly the dietary intervention, has been critiqued for differences in the composition of diets between groups. 51 For example, the high-fat control diet and the HFD supplemented with black beans contained 7.8 and 0 g of sucrose (per 100 g), respectively. The differences in sucrose between was critiqued due to the possibility of consequential changes to intestinal microbiota from high sucrose levels in the diet.…”

“…The differences in sucrose between was critiqued due to the possibility of consequential changes to intestinal microbiota from high sucrose levels in the diet. 51,52 It must be noted that to measure gut microbiota composition and function, some studies use DNA from cecal contents collected at necropsy, while others extract DNA from fecal samples. Diversity and composition of the microbiome can differ depending on the location of the gastrointestinal tract, 53 meaning location of the microbiota samples should be taken into consideration when comparing results.…”

“…The fat content of the HFD was not reported. This study design, particularly the dietary intervention, has been critiqued for differences in the composition of diets between groups 51 . For example, the high‐fat control diet and the HFD supplemented with black beans contained 7.8 and 0 g of sucrose (per 100 g), respectively.…”

“…For example, the high‐fat control diet and the HFD supplemented with black beans contained 7.8 and 0 g of sucrose (per 100 g), respectively. The differences in sucrose between was critiqued due to the possibility of consequential changes to intestinal microbiota from high sucrose levels in the diet 51,52 …”

Reply: “Comment on ”Consumption of Cooked Black Beans Stimulates a Cluster of Some Clostridia Class Bacteria Decreasing Inflammatory Response and Improving Insulin Sensitivity.” Nutrients 2020, 12(4), 1182”

“…We appreciate the interest of Dr. Cervantes for his/her comments [1] on our article [2] and for providing us with the opportunity to extend the discussion regarding diet composition in our interpretation of the findings of gut microbiota and fecal butyrate concentration. The following is our response to his/her concerns.…”