Sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process

Sasaki, Kengo; Tsuge, Yota; Kawaguchi, Hideo; Yasukawa, Masahiro; Sasaki, Daisuke; Sazuka, Takashi; Kamio, Eiji; Ogino, Chiaki; Matsuyama, Hideto; Kondo, Akihiko

doi:10.1007/s00253-017-8316-3

Cited by 15 publications

(7 citation statements)

References 22 publications

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“…43 Repeated use of cells for multiple production cycles reduces the costs and time of downstream processing. 44,45 The use of an oxygen-responsive metabolic switch demonstrated in the current study offers a new approach for an efficient production of compounds for improved titer and yield, while also saving the production costs and time by allowing a repeated use of cells for aerobic and anaerobic production.…”

Section: Modulation Of the Split Ratio Of Carbon Flux Between Glycoly...mentioning

confidence: 99%

Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum

et al. 2020

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Controlling the carbon flux into a desired pathway is important for improving product yield in metabolic engineering. After entering a cell, glucose is channeled into glycolysis and the pentose phosphate pathway (PPP), which decreases the yield of target products whose synthesis relies on NADPH as a cofactor. Here, we demonstrate redirection of carbon flux into PPP under aerobic conditions in Corynebacterium glutamicum, achieved by replacing the promoter of glucose 6-phosphate isomerase gene (pgi) with an anaerobic-specific promoter of the lactate dehydrogenase gene (ldhA). The promoter replacement increased the split ratio of carbon flux into PPP from 39 to 83% under aerobic conditions. The titer, yield, and production rate of 1,5-diaminopentane, whose synthesis requires NADPH as a cofactor, were increased by 4.6-, 4.4-, and 2.6-fold, respectively. This is the largest improvement in the production of 1,5-diaminopentane or its precursor, lysine, reported to date. After aerobic cell growth, pgi expression was automatically induced under anaerobic conditions, altering the carbon flux from PPP to glycolysis, to produce succinate in a single metabolically engineered strain. Such an automatic redirection of metabolic pathway using an oxygen-responsive switch enables two-stage fermentation for efficient production of two different compounds by a single strain, potentially reducing the production costs and time for practical applications.

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Section: Modulation Of the Split Ratio Of Carbon Flux Between Glycoly...mentioning

confidence: 99%

Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum

et al. 2020

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“…In this study, wildtype and no.7 displayed around 8.5~10.2 g•L −1 and 92% as the yield and percent yield of ethanol. According to previous reports [23], the ethanol fermentation of BY4741 is 104.4 ± 3.1 g•L −1 , and the percent yield of ethanol was 84.4-89.6%. Comparing our results with the previously reported ones, the values of ethanol fermentation in this study were better or higher.…”

Section: Time-course Profiles Of Carbon Source Consumption and Ethano...mentioning

confidence: 60%

Estimation of Carbon Metabolism in Saccharomyces cerevisiae Acclimatized to Glycerol Assimilation with Quantitative PCR

2022

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Saccharomyces cerevisiae has the potential to produce value-added chemicals; however, this strain is restricted by using glycerol as a carbon source. Although acclimatization of S. cerevisiae as a glycerol-assimilating strain was confirmed so far, the reason why S. cerevisiae can be acclimatized was not clear in detail with limited information on the metabolic changes. In this report, glycerol-assimilating strains from S. cerevisiae BY4741 were isolated, and the biomass production, ethanol fermentation, and transcription levels related to glycolysis and the tricarboxylic acid cycle under aerobic and slightly anaerobic conditions were analyzed. As the results show, although µmax was equal to 0.15 h−1 between wildtype and glycerol-assimilating strains in an aerobic culture including glucose, the differences in max biomass production and percentage yields of ethanol and transcription levels between the two strains were shown. In slightly anaerobic culture, the differences in transcription levels downstream of glycolysis were also displayed. In the case of the glycerol-assimilating strain with glycerol under aerobic conditions, although the transcription levels related to ethanol production were sufficient, the ethanol production was not detected. Additionally, the biomass production reached a plateau even in the culture containing sufficient glycerol, indicating that the redox imbalance even in the cells of the glycerol-acclimatized strain could disturb the utilization of glycerol. The obtained knowledge will promote the use of glycerol resources with the glycerol-acclimatized S. cerevisiae in view of carbon recycling.

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“…The primary hydrolysate from alkali-pretreated EFB using concentrated XYN10Ks_480 endoxylanase produced by recombinant S. lividans 1326 was subjected to sequential membrane NFs to refine and concentrate the XOS from xylose. The liquor was first subjected to an RS50 UF membrane to remove the macromolecules and to recover the sugar solution [28]. The UF permeate contained 8 g.L −1 xylose and 11.2 g.L −1 xylobiose (xylopentaose was a minuscule fraction, less than 1 g.L −1 ).…”

Section: Concentration and Separation Of Xylobiose By Nanofiltrationmentioning

confidence: 99%

High Enzymatic Recovery and Purification of Xylooligosaccharides from Empty Fruit Bunch via Nanofiltration

et al. 2020

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Xylooligosaccharides (XOS) are attracting an ever-increasing amount of interest for use as food prebiotics. In this study, we used efficient membrane separation technology to convert lignocellulosic materials into a renewable source of XOS. This study revealed a dual function of nanofiltration membranes by first achieving a high yield of xylobiose (a main component of XOS) from alkali-pretreated empty fruit bunch (EFB) hydrolysate, and then by achieving a high degree of separation for xylose as a monosaccharide product. Alkali pretreatment could increase the xylan content retention of raw EFB from 23.4% to 26.9%, which eventually contributed to higher yields of both xylobiose and xylose. Nanofiltration increased the total amount of XYN10Ks_480 endoxylanase produced from recombinant Streptomyces lividans 1326 without altering its specific activity. Concentrated XYN10Ks_480 endoxylanase was applied to the recovery of both xylobiose and xylose from alkali-pretreated EFB hydrolysate. Xylobiose and xylose yields reached 41.1% and 17.3%, respectively, and when unconcentrated XYN10Ks_480 endoxylanase was applied, those yields reached 35.1% and 8.3%, respectively. The last step in nanofiltration was to separate xylobiose over xylose, and 41.3 g.L−1 xylobiose (90.1% purity over xylose) was achieved. This nanofiltration method should shorten the processes used to obtain XOS as a high-value end product from lignocellulosic biomass.

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Sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process

Cited by 15 publications

References 22 publications

Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum

Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum

Estimation of Carbon Metabolism in Saccharomyces cerevisiae Acclimatized to Glycerol Assimilation with Quantitative PCR

High Enzymatic Recovery and Purification of Xylooligosaccharides from Empty Fruit Bunch via Nanofiltration

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