Sucrose synthase activity and carbohydrates content in relation to phosphorylation status of Vicia faba root meristems during reactivation from sugar depletion

Polit, Justyna Teresa; Ciereszko, Iwona

doi:10.1016/j.jplph.2012.04.017

Cited by 15 publications

(10 citation statements)

References 44 publications

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“…6 and 7), we could suppose that both forms of intracellular invertase were active. This assumption is confirmed by data identifying the cytoplasmic invertase activity in the root cells of Vicia faba [19]. Indeed, biochemical analysis of various forms of invertase showed a small unreliable difference between the activity of the vacuolar and cytoplasmic invertases in the meristematic zone.…”

Section: Discussionmentioning

confidence: 53%

“…The above mentioned data and also the absence of staining in the control sections performed without sucrose in the incubation medium characterize the applicability of the method used for the detection of the enzyme activity. Some other authors use this technique to analyze the activity of invertase even in the root tips [14,19].…”

Section: Intracellular Localization Of Invertasementioning

confidence: 99%

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Histochemical Examination of Invertase Activities in the Growing Tip of the Plant Root

Lunkova¹,

Бурмистрова²,

Krasavina³

2017

TOPSJ

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Background:A growing part of the root is one of the most active sinks for sucrose coming from source leaves through the phloem. In the root, sucrose is unloaded from conducting bundles and is distributed among the surrounding cells. To be involved in the metabolism, sucrose should disintegrate into hexoses by means of degrading enzymes. Aims:The aim of this research was to explore the possibility of the involvement of one such enzymes, invertase, in phloem unloading as well as distribution of its activity in the functionally different tissues of the plant root tips. Method:To estimate the enzyme activities in root tissues, we applied two techniques: the histochemical method using nitro blue tetrazolium. The localization of phloem unloading was studied with carboxyfluorescein, a fluorescent marker for symplastic transport. Results:Invertase activity was not detected in the apical part of the meristem. It appeared only between the basal part of this zone and the beginning of the elongation zone. There is the root phloem unloading in that area. Invertase activity increased with increasing the distance from the root tip and reached the highest values in the region of cell transition to elongation and in the elongation zone. The activities of the enzyme varied in different tissues of the same zone and sometimes in the neighboring cells of the same tissue. Biochemical determination of invertase activity was made in the maize root segments coincident to the zones of meristem, cell elongation and differentiation. The results of both methods of determination of invertase activity were in agreement. Conclusion:It was concluded that phloem unloading correlated with invertase activity, possibly because of the activation of invertase by unloaded sucrose. Invertase is one of the factors involved in the processes preparing the cells for their transition to elongation because the concentration of osmotically active hexoses increases after cleavage of sucrose, that stimulates water entry into the cells, which is necessary for elongation growth.

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Section: Discussionmentioning

confidence: 53%

Section: Intracellular Localization Of Invertasementioning

confidence: 99%

Histochemical Examination of Invertase Activities in the Growing Tip of the Plant Root

Lunkova¹,

Бурмистрова²,

Krasavina³

2017

TOPSJ

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“…Phosphorus is a critical macronutrient required for plant development and metabolism, including processes such as organic compound biosynthesis, photosynthesis, respiration, energy transfer, coordination of gene expression and enzymes activity, or signal transduction [1][2][3][4][5]. The P availability is one of the most limiting factors for crop plant growth and yield production, mainly due to its immobilization.…”

Section: Introductionmentioning

confidence: 99%

“…Phosphorus in soil exists predominantly as sparingly soluble inorganic phosphates and insoluble organic phosphates, which are not directly available to plants. The available forms of P are H 2 PO 4 − and HPO 3 − ions (Pi), usually present in the soil solution in very small concentrations [6]. Plants have evolved various morphological, physiological, and biochemical adaptations to counteract phosphate deficiency.…”

Section: Introductionmentioning

confidence: 99%

“…Alternatively, plants are developing specialized cluster/proteoid roots or can activate root colonization by mycorrhizal fungi and/or rhizosphere bacteria [5,12,[18][19][20][21]. Phosphate starvation often affects metabolic processes in shoots, e.g., photosynthesis and respiration or causes alterations in the partitioning of assimilated carbon into sucrose/starch pathways through the regulation of gene expression and/or protein phosphorylation [2,4,22,23]. Most of these reactions of plants are believed to be a systemic response, including metabolic adjustments for more efficient Pi recycling under deficient conditions [14,16,24].…”

Section: Introductionmentioning

confidence: 99%

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The effects of diversified phosphorus nutrition on the growth of oat (Avena sativa L.) and acid phosphatase activity

et al. 2018

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We studied the effect of differential phosphorus (P) supply on the development of oat seedlings (Avena sativa L. ' Arab') as well as localization and activity of acid phosphatases in tissues and root exudates. Plants were grown for 1-3 weeks on nutrient media with inorganic phosphate (+P, control), reduced Pi (0.1 P), phytic acid (PA) as organic P source, and without P addition (−P), in standard conditions or in a split-root culture system. Phosphate starvation reduced shoot growth but increased root elongation and root/shoot ratio, whereas 0.1 P and PA oat plants had similar growth parameters to +P plants. The growth on −P medium significantly decreased Pi content in all tissues, but only a slight Pi decrease was observed in plants grown on 0.1 P and PA media or various split-root system conditions. Pi starvation led to an increase in acid phosphatase (APase) activity in root exudates when compared to +P, 0.1 P, and PA plant samples. APase activity was especially intensive in root cross sections in rhizodermis and around/in vascular tissues of −P plants. For plants grown on 0.1 P medium and on phytic acid, APase activity did not change when compared to the control. Three major isoforms of APases were detected in plant tissues (similar in all studied conditions, with a higher activity of one isoform under Pi deficit). Generally, lowered Pi content (0.1 P) was not stressful to oat plants for up to 3 weeks of culture. Oat plants grew equally well on nutrient media with Pi and on media with phytate, although phytate was considered not available for other plants. The oat plants activated mainly extracellular APases, but also intracellular enzymes, rather via nonlocal signals, to acquire Pi from external/ internal sources under Pi deficiency.

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Early‐stage sugar beet taproot development is characterized by three distinct physiological phases

et al. 2020

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Despite the agronomic importance of sugar beet ( Beta vulgaris L.), the early‐stage development of its taproot has only been poorly investigated. Thus, the mechanisms that determine growth and sugar accumulation in sugar beet are largely unknown. In the presented study, a physiological characterization of early‐stage sugar beet taproot development was conducted. Activities were analyzed for fourteen key enzymes of carbohydrate metabolism in developing taproots over the first 80 days after sowing. In addition, we performed in situ localizations of selected carbohydrate‐metabolic enzyme activities, anatomical investigations, and quantifications of soluble carbohydrates, hexose phosphates, and phytohormones. Based on the accumulation dynamics of biomass and sucrose, as well as on anatomical parameters, the early phase of taproot development could be subdivided into three stages—prestorage, transition, secondary growth and sucrose accumulation stage—each of which was characterized by distinct metabolic and phytohormonal signatures. The enzyme activity signatures corresponding to these stages were also shown to be robustly reproducible in experiments conducted in two additional locations. The results from this physiological phenotyping approach contribute to the identification of the key regulators of sugar beet taproot development and open up new perspectives for sugar beet crop improvement concerning both physiological marker‐based breeding and biotechnological approaches.

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Sucrose synthase activity and carbohydrates content in relation to phosphorylation status of Vicia faba root meristems during reactivation from sugar depletion

Cited by 15 publications

References 44 publications

Histochemical Examination of Invertase Activities in the Growing Tip of the Plant Root

Histochemical Examination of Invertase Activities in the Growing Tip of the Plant Root

The effects of diversified phosphorus nutrition on the growth of oat (Avena sativa L.) and acid phosphatase activity

Early‐stage sugar beet taproot development is characterized by three distinct physiological phases

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