Sucrose Synthase Expression during Cold Acclimation in Wheat

Abstract

When wheat (Triticum aestivum) seedlings are exposed to a cold temperature (2-40C) above 00C, sucrose accumulates and sucrose synthase activity increases. The effect of a cold period on the level of sucrose synthase (SS) was investigated. Using antibodies against wheat germ SS, Western blots studies showed that the amount of the SS peptide increased during 14 days in the cold, when plants were moved from 230C to 40C. The level of SS diminished when plants were moved back to 230C. Northern blots of poly(A)+ RNA, confirmed a five-to sixfold induction of SS in wheat leaves during cold acclimation. These results indicate that SS is involved in the plant response to a chilling stress.

Molecular Cloning of Abscisic Acid-Responsive mRNAs Expressed during the Induction of Freezing Tolerance in Bromegrass (Bromus inermis Leyss) Suspension Culture

“…It has been suggested that there is a requirement for the up-regulation of genes involved with sugar metabolism during cold acclimation (Guy, 1990). Evidence for this was demonstrated in winter wheat, where Suc synthase polypeptide and poly(A)+ RNA levels increased with lowtemperature treatment (Crespi et al, 1991). Results of this study also suggest that a phosphoglucomutase gene, encoding an enzyme of the glycolysis/gluconeogenesis pathway, has a higher leve1 of expression during ABA-induced hardening.…”

Differential Interactions of Promoter Elements in Stress Responses of the Arabidopsis Adh Gene

“…More recently it was shown that another maize anaerobic protein, Suc synthase, is also inducible by low-temperature conditions (Crespi et al, 1991). Although it is not known whether a11 maize anaerobically inducible genes are also induced by cold stress, the question arises whether regulation of gene expression by both environmental stresses (anaerobiosis and cold) are mediated through the same signal-transduction pathway .…”

Detection of Sugar Accumulation and Expression Levels of Correlative Key Enzymes in Winter Wheat (Triticum aestivum) at Low Temperatures

“…15) In contrast, regulation of the enzymes involved in sucrose synthesis is more complicated under cold stress, 16) often giving conflicting results when various studies are compared. 17,18) We are also interested in the transport of fixed carbon from photosynthesis and the level of glycolysis in cells. The expression levels of two key enzymes, triose phosphate translocator (TPT) and pyruvate kinase (PK; EC 2.7.1.40) have been determined.…”