Sucrose ‘Versus’ Trehalose Cryoprotectant Modification in Oocyte Vitrification : A Study of Embryo Development

Lestari, Silvia Werdhy; Ilato, Khairunnisa F.; Pratama, Muhammad Iqbal Adi; Fitriyah, Nurin N.; Pangestu, Mulyoto; Pratama, Gita; Margiana, Ria

doi:10.13005/bpj/1351

Cited by 10 publications

(10 citation statements)

References 29 publications

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“…It is used alone or associated with an internal cryoprotectant. Di- and trisaccharides are well known external cryoprotectants, mainly employed with success in the vitrification of mammalian cells and embryos (Eroglu et al., 2002; Kuwayama et al., 2005; Emeka 2015; Lestari et al., 2018). Sucrose, trehalose, or raffinose have been usually employed at concentrations (mean 500 mmol) that share a high osmotic effect in order to increase the dehydration of the cells during freezing; and in embryos, to decrease the osmotic shocks at thawing.…”

Section: Discussionmentioning

confidence: 99%

Sucrose increases the quality and fertilizing ability of cryopreserved chicken sperms in contrast to raffinose

Thananurak¹,

Chuaychu-noo²,

Thélie³

et al. 2019

Poultry Science

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Chicken semen conservation is an important tool for programs of genetic diversity management and of endangered breeds’ conservation. However, the method still needs to be improved in order to be applied in a wide variety of environments and breeds. Our objective was to compare the effects of 2 external cryoprotectants saccharides (sucrose and raffinose) on the sperm freezability of a Thai local breed, Pradu Hang Dum, in which semen was frozen with a simple freezing method using nitrogen vapors and dimethyl formamide (DMF). Thirty-six males were selected on their motility vigor score for the experiments. In a first experiment, a large range of sucrose and raffinose doses were tested. Semen quality was evaluated after incubation at 5°C or after cryopreservation in straws in the saline Blumberger Hahnen Sperma Verdünner diluent + DMF (6% v/v) with or without sucrose/raffinose. The best targeted doses of sucrose and raffinose were then kept for experiment 2 that was focused on cryopreserved semen. In this experiment, semen quality was measured on frozen-thawed sperm: different objective motility data evaluated by computer-assisted sperm analysis (CASA), membrane integrity, acrosome integrity, mitochondria function evaluated using flow cytometry, lipid peroxide production assessed by the thiobarbituric acid test. Fertility obtained with frozen-thawed semen supplemented or not with sucrose or raffinose was also evaluated after artificial insemination of laying hens. The presence of sucrose at the osmotically inactive dose 1 mmol significantly increased the vigor motility, membrane integrity, acrosome integrity, and mitochondrial functions of frozen-thawed sperm ( P < 0.05), and showed the highest levels of fertility after sperm cryopreservation (91% vs. control 86%, P < 0.001). Raffinose showed negative effects on in vitro semen quality from 1 to 100 mmol. Fertility was also negatively ( P < 0.001) affected by raffinose (fertility rate 66 to 70%). We thus showed in the present study the high success of a simple chicken sperm cryopreservation method with an external cryoprotectant easily available and cheap, the sucrose, used at an osmotically inactive low concentration.

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Section: Discussionmentioning

confidence: 99%

Sucrose increases the quality and fertilizing ability of cryopreserved chicken sperms in contrast to raffinose

Thananurak¹,

Chuaychu-noo²,

Thélie³

et al. 2019

Poultry Science

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“…It is, therefore, clear that significantly lower amounts of viable and motile sperms are found in cigarette smokers compared to cigarette non-smokers. [1] In furthering, the evidence of the degree of damage that smoking causes on sperms, the studies highlighted surmountable evidence that a higher number of cigarette smokers are asthenozoospermic.…”

Section: Discussionmentioning

confidence: 99%

“…The structure of the sperm DNA keeps nuclear chromatins compact and highly stable; conversely, pro-mutagenic change is caused by DNA damage which in severe degrees affect the germline quality, preventing embryo development. [1] Studies reveal high levels of negative association between multiple reproductive indices such as the rate of fertilization, embryo cleavage, implantation, and the degree of DNA fragmentation or damage. Furthermore, researchers established recently that severe damage to spermatozoa DNA may be consequential to recurrent spontaneous abortion.…”

Section: Introductionmentioning

confidence: 99%

Untitled

Margiana¹

2018

AJP

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Background: The negative deleterious effects of cigarette smoking are widely known, especially the relationship its influence on semen quality and sperm DNA fragmentation. Male cigarette smoking was highlighted and hypothesized to not only affect cause DNA fragmentation but also affect the early embryo development. To summarize various literature studies, a systematic review of relevant observational studies was conducted to determine the relationship between cigarette smoking among men and DNA fragmentation and the ultimate influence on embryo development. Methods: A methodical explore of the observational literature on the subject was carried out on Google Scholar. All papers relevant observational papers reporting on the tie of cigarette smoking with sperm DNA fragmentation and embryo growth were included. The retrieval of pertinent reports was done and subsequently the systematic search of reference lists to help in the identification of studies qualifying for inclusion. Results: Of the 14 studies, 4 papers were retrieved reporting observational findings that cigarette smoking causes adverse DNA damage which is evidentially correlated with poor embryo growth. However, the remaining studies had no information implying any existent relationship between cigarette smoking, DNA damage, and development of the embryo. Conclusions: The literature asserts that cigarette smoking through sperm DNA fragmentation affects early embryonic development. Well-designed research literature with evidence-based, predefined criteria for subject selection, the definition of cigarette smoking, and its contents are essential in achieving consistent affirmation on the result of smoking on DNA fragmentation and ultimately, embryo development.

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“…However, the highest vitrification efficiency for immature horse oocytes was reached when a vitrification medium containing trehalose was used (42% maturation (10/24), 80% cleavage (8/10) and 10% blastocysts (1/10)), whereas no blastocysts were obtained from sucrose-containing media (Canesin et al 2017). In humans and mice, oocyte vitrification in trehalose-containing medium is also associated with higher blastocyst rates than vitrification with sucrose (Coello et al 2016), but the difference is not always statistically significant Lestari et al 2018), probably due to variations in the protocols used. Injection of sugars into the oocyte can avoid osmotic stress associated with the use of non-permeating CPAs (Woelders et al 2018).…”

Section: Equilibration Solutionmentioning

confidence: 99%

Cryopreservation of equine oocytes: looking into the crystal ball

Coster

Angel‐Velez

Soom

et al. 2020

Reprod. Fertil. Dev.

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In vitro embryo production has evolved rapidly in the horse over the past decade, but blastocyst rates from vitrified equine oocytes remain quite poor and further research is needed to warrant application. Oocyte vitrification is affected by several technical and biological factors. In the horse, short exposure of immature oocytes to the combination of permeating and non-permeating cryoprotective agents has been associated with the best results so far. High cooling and warming rates are also crucial and can be obtained by using minimal volumes and open cryodevices. Vitrification of in vivo-matured oocytes has yielded better results, but is less practical. The presence of the corona radiata seems to partially protect those factors that are necessary for the construction of the normal spindle and for chromosome alignment, but multiple layers of cumulus cells may impair permeation of cryoprotective agents. In addition to the spindle, the oolemma and mitochondria are also particularly sensitive to vitrification damage, which should be minimised in future vitrification procedures. This review presents promising protocols and novel strategies in equine oocyte vitrification, with a focus on blastocyst development and foal production as most reliable outcome parameters.

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Sucrose ‘Versus’ Trehalose Cryoprotectant Modification in Oocyte Vitrification : A Study of Embryo Development

Cited by 10 publications

References 29 publications

Sucrose increases the quality and fertilizing ability of cryopreserved chicken sperms in contrast to raffinose

Sucrose increases the quality and fertilizing ability of cryopreserved chicken sperms in contrast to raffinose

Untitled

Cryopreservation of equine oocytes: looking into the crystal ball

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