Suction Strainer Technique in Obtaining Rumen Fluid Samples from Intact Lambs

Raun, Ned S.; Burroughs, Wise

doi:10.2527/jas1962.213454x

Cited by 135 publications

(56 citation statements)

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“…On d 16 of each period, ruminal fluid samples were collected every 3 h starting at 0900 using the suction strainer technique (Raun and Burroughs, 1962). Ruminal fluid samples (50 mL) were immediately measured for pH using a combination electrode and then frozen (−20°C) for further analysis.…”

Section: Methodsmentioning

confidence: 99%

Effect of corn processing on starch digestion and bacterial crude protein flow in finishing cattle

Cooper

Milton

Klopfenstein

et al. 2002

Journal of Animal Science

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Six ruminally and duodenally cannulated yearling steers (523 kg) were used in a replicated 3 × 3 Latin square design experiment to study the effects of corn processing on nutrient digestion, bacterial CP production, and ruminal fermentation. Dietary treatments consisted of 90% concentrate diets that were based on dry-rolled (DRC), high-moisture (HMC), or steam-flaked (SFC) corn. Each diet contained 2.0% urea (DM basis) as the sole source of supplemental nitrogen. Each period lasted 17 d, with d 1 through 14 for diet adaptation and d 15 through 17 for fecal, duodenal, and ruminal sampling. Dry matter and OM intakes were similar for DRC and SFC but were approximately 15% higher (P < 0.05) for HMC. True ruminal OM digestibilities were 18 and 10% greater (P < 0.05) for HMC than for DRC or SFC, respectively. Ruminal starch digest-

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Section: Methodsmentioning

confidence: 99%

Effect of corn processing on starch digestion and bacterial crude protein flow in finishing cattle

Cooper

Milton

Klopfenstein

et al. 2002

Journal of Animal Science

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“…Labeling procedures for corn and forage were the same as outlined by Sindt et al (1993). Samples of ruminal fluid and particulate matter were taken at 0 h and at 6-h intervals for 24 h following dosing using the suction strainer technique (Raun and Burroughs, 1962). Particulate matter samples were a composite of samples taken Table 3.…”

Section: Metabolism Trialmentioning

confidence: 99%

The effect of forage source and particle size on finishing yearling steer performance and ruminal metabolism.

Shain

Stock

Klopfenstein

et al. 1999

Journal of Animal Science

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ABSTRACT:Two finishing trials and a metabolism trial were conducted to evaluate the effect of forage source and particle size in dry-rolled corn finishing diets. In Exp. 1, 224 crossbred yearling steers (BW = 342 ± 11 kg) were used in a randomized complete block design consisting of seven treatments. Treatments were an all-concentrate diet or diets containing equal NDF levels provided by alfalfa hay or wheat straw (three treatments each) with each forage source ground to pass through a .95-, 7.6-, or 12.7-cm screen. Steers fed diets containing forage had greater ( P < .05) DMI than steers fed an allconcentrate diet. Steers fed alfalfa diets gained faster ( P < .05) with a greater ( P < .05) concentrate efficiency than steers fed either all-concentrate or straw diets. In Exp. 2, 120 crossbred yearling steers (BW = 307 ± 2 kg) were used in a completely randomized design and fed dry-rolled corn diets containing 10% alfalfa ground to pass through either a .95-or 7.6-cm screen. Alfalfa particle size had no effect on performance or carcass measurements. In Exp. 3, six ruminally fistulated steers (BW = 508 ± 34 kg) were used in a 6 × 6 Latin square design and fed an all-concentrate diet or diets containing equal NDF levels provided by alfalfa hay, wheat straw, or ground corncobs with alfalfa and straw ground to pass through either a 2.54-or 12.7-cm screen. Steers fed straw diets spent more time ( P < .10) chewing than those receiving the other diets. In conclusion, forage particle size had no effect on finishing cattle performance or ruminal metabolism data. However, cattle consuming different forage sources in dry-rolled corn finishing diets may not respond similarly in animal performance.

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“…No entanto, na etapa de coleta do conteúdo ruminal para inocular as amostras de alimento, é fundamental que sejam preservadas as mesmas condições ruminais, o que exige uma retirada criteriosa e rápida. Dependendo do método de coleta, poderão ocorrer desvantagens que irão interferir nos resultados, principalmente dos parâmetros ruminais, tais como: pH, temperatura, concentração de ácidos graxos voláteis e nitrogênio amoniacal (Raun & Burroughs, 1962;Lane et al, 1966;Wolt et al, 1976;Ortolani, 1981;Kolb, 1984;Lavezzo et al, 1988) e microbiológicos, como o número de protozoários no fluido ruminal. Ainda podem ser citadas como fontes de variação o local de amostragem no rúmen e a diluição do conteúdo ruminal decorrente da ingestão de alimentos, de saliva e de água (Hungate, 1966;Lane et al, 1968;Mukherjee & Sinha, 1990, citados por Freitas, 1995.…”

Section: Introductionunclassified

“…Por outro lado, o método de coleta por meio da sonda esofageana poderia contaminar o conteúdo com excesso de saliva, proporcionando, com isso, elevação no pH (Raun & Burroughs, 1962;Ortolani, 1981) e diminuição nas concentrações de nitrogênio amoniacal (Wolt et al, 1976) e de ácidos graxos voláteis (Raun & Burroughs, 1962). Segundo Lane et al (1968), a análise do conteúdo ruminal proveniente de um só local proporcionou uma idéia irreal das condições prevalentes no rúmen.…”

Section: Introductionunclassified

“…Ainda que os métodos de coleta de conteúdo ruminal, via sonda, proporcionem alterações nos parâmetros ruminais, segundo Raun & Burroughs (1962), quanto ao pH e ácidos graxos voláteis, as diferenças não foram relevantes. Todavia, a proporção molar do ácido acético foi maior em amostras coletadas pelo método manual em relação ao da sonda esofageana (Putnam et al, 1969).…”

Section: Introductionunclassified

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Efeito de métodos de coleta de fluido ruminal em bovinos sobre alguns parâmetros ruminais e microbiológicos

Oliveira

Sampaio

Vieira

et al. 1999

Pesq. agropec. bras.

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RESUMO -Estudou-se o efeito de três métodos de coleta (manual, bomba a vácuo e sonda nasoesofageana) sobre os teores de ácidos graxos voláteis totais (AGVt), nitrogênio amoniacal e número de protozoários no fluido ruminal de bovino. Utilizou-se o delineamento inteiramente casualizado, com três tratamentos e cinco repetições. Os resultados revelaram que o método da sonda nasoesofageana proporcionou valores menores dos ácidos propiônico, butírico, AGVt e de nitrogênio amoniacal no fluido ruminal. O número total de protozoários não foi afetado pelos métodos de coleta de conteúdo ruminal. O método de coleta manual e por meio de bomba a vácuo mostraram-se eficientes para os parâmetros estudados.Termos para indexação: ácido propiônico, bomba a vácuo, nitrogênio amoniacal, protozoários. EFFECT OF COLLECTION METHODS OF BOVINE RUMINAL FLUID ON RUMINAL AND MICROBIOLOGICAL PARAMETHERSABSTRACT-An experiment was conducted to determine the effects of three methods of collection on volatile fatty acids concentrations, amoniacal nitrogen levels and protozoa number in ruminal fluid of bovine. Three methods of collection were used: manual, vacuum-pump and with a nasal-esophagic tube. It was used a completely randomized design with three treatments and five replications. The data showed that the nasal-esophagic tube method resulted in lower values of propionic acid, butiric acid, volatile fatty acids and amoniacal nitrogen in ruminal fluid. The total number of protozoa do not affected by the methods of fluid collection. The manual and the vacuum-pump method showed an indicated methods for the determinations of the studied parameters.

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Suction Strainer Technique in Obtaining Rumen Fluid Samples from Intact Lambs

Cited by 135 publications

References 0 publications

Effect of corn processing on starch digestion and bacterial crude protein flow in finishing cattle

Effect of corn processing on starch digestion and bacterial crude protein flow in finishing cattle

The effect of forage source and particle size on finishing yearling steer performance and ruminal metabolism.

Efeito de métodos de coleta de fluido ruminal em bovinos sobre alguns parâmetros ruminais e microbiológicos

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