Suffruticosol A elevates osteoblast differentiation targeting <scp>BMP2‐Smad</scp>/1/5/<scp>8‐RUNX2</scp> in pre‐osteoblasts

Yun, Hyung-Mun; Kim, Bomi; Jeong, Yun Hee; Hong, Jin Tae; Park, Kyung-Ran

doi:10.1002/biof.1878

Cited by 6 publications

(10 citation statements)

References 67 publications

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“…By stimulating osteoblast differentiation, anabolic medicines derived from natural compounds can be utilized to treat and prevent bone diseases [ 23 , 24 , 25 , 26 ]. Recently, we reported the beneficial effects of various natural compounds isolated from plants on osteogenic cells [ 3 , 26 , 27 , 28 , 29 , 30 , 31 ]. For osteoblast differentiation in in vitro research, native bone marrow-derived osteoblasts and other cell sources were studied [ 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Vanillin Promotes Osteoblast Differentiation, Mineral Apposition, and Antioxidant Effects in Pre-Osteoblasts

Yun,

Kim,

Kwon

et al. 2024

Pharmaceutics

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Antioxidant vanillin (4-hydroxy-3-methoxybenzaldehyde) is used as a flavoring in foods, beverages, and pharmaceuticals. Vanillin possesses various biological effects, such as antioxidant, anti-inflammatory, antibacterial, and anticancer properties. This study aimed to investigate the biological activities of vanillin purified from Adenophora triphylla var. japonica Hara on bone-forming processes. Vanillin treatment induced mineralization as a marker for mature osteoblasts, after stimulating alkaline phosphatase (ALP) staining and activity. The bone-forming processes of vanillin are mainly mediated by the upregulation of the bone morphogenetic protein 2 (BMP2), phospho-Smad1/5/8, and runt-related transcription factor 2 (RUNX2) pathway during the differentiation of osteogenic cells. Moreover, vanillin promoted osteoblast-mediated bone-forming phenotypes by inducing migration and F-actin polymerization. Furthermore, we validated that vanillin-mediated bone-forming processes were attenuated by noggin and DKK1. Finally, we demonstrated that vanillin-mediated antioxidant effects prevent the death of osteoblasts during bone-forming processes. Overall, vanillin has bone-forming properties through the BMP2-mediated biological mechanism, indicating it as a bone-protective compound for bone health and bone diseases such as periodontitis and osteoporosis.

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Section: Discussionmentioning

confidence: 99%

Vanillin Promotes Osteoblast Differentiation, Mineral Apposition, and Antioxidant Effects in Pre-Osteoblasts

Yun,

Kim,

Kwon

et al. 2024

Pharmaceutics

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“…The P838 voucher specimen ( P. suffruticosa dried fruit) was deposited at the Natural Products Bank (NIKOM, Gyeongsan, Korea). The isolation of Suffruticosol B (Suf-B) was carried out in the same experimental procedure as previously described [ 63 ], and 82 mg Suf-B (99.99%, brown powder, C 42 H 32 O 9 ) was isolated from 130.0 g P. suffruticosa dried fruit.…”

Section: Methodsmentioning

confidence: 99%

“…The MTT assay was performed as previously described [ 63 ]. Briefly, MTT (Sigma-Aldrich) solution (5 mg/mL stock in 1× PBS) was directly added to MC3T3-E1 pre-osteoblasts for 2 h. Formazan was dissolved in 200 μL of 100% dimethyl sulfoxide (DMSO) (Sigma-Aldrich), and the absorbance was detected at a 540 nm wavelength in a Multiskan GO Microplate Spectrophotometer (Thermo Fisher Scientific).…”

Section: Methodsmentioning

confidence: 99%

“…ALP staining and activity were analyzed by using an ALP reaction solution (Takara Bio Inc., Tokyo, Japan) and ALP activity colorimetric assay kit (Biovision, Milpitas, CA, USA), respectively, as previously described [ 63 ]. ARS was analyzed by using a 2% ARS (Sigma-Aldrich) solution (pH 4.2) to monitor mineralization, as previously described [ 49 , 63 ].…”

Section: Methodsmentioning

confidence: 99%

“…An autophagy detection kit (Dojindo, Kumamoto, Japan) was used to monitor autophagosome formation, which was observed by using a fluorescence microscope. Autophagic signaling proteins were detected by Western blot analysis, as described previously [ 63 , 66 ].…”

Section: Methodsmentioning

confidence: 99%

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Suffruticosol B Is an Osteogenic Inducer through Osteoblast Differentiation, Autophagy, Adhesion, and Migration

Yun

Lee

Kim

et al. 2022

IJMS

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Suffruticosol B (Suf-B) is a stilbene found in Paeonia suffruticosa ANDR., which has been traditionally used in medicine. Stilbenes and their derivatives possess various pharmacological effects, such as anticancer, anti-inflammatory, and anti-osteoporotic activities. This study aimed to explore the bone-forming activities and mechanisms of Suf-B in pre-osteoblasts. Herein, >99.9% pure Suf-B was isolated from P. suffruticosa methanolic extracts. High concentrations of Suf-B were cytotoxic, whereas low concentrations did not affect cytotoxicity in pre-osteoblasts. Under zero levels of cytotoxicity, Suf-B exhibited bone-forming abilities by enhancing alkaline phosphatase enzyme activities, bone matrix calcification, and expression levels with non-collagenous proteins. Suf-B induces intracellular signal transduction, leading to nuclear RUNX2 expression. Suf-B-stimulated differentiation showed increases in autophagy proteins and autophagosomes, as well as enhancement of osteoblast adhesion and transmigration on the ECM. These results indicate that Suf-B has osteogenic qualities related to differentiation, autophagy, adhesion, and migration. This also suggests that Suf-B could have a therapeutic effect as a phytomedicine in skeletal disorders.

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A Biomimetic Multifunctional Scaffold for Infectious Vertical Bone Augmentation

Zhang,

Li,

Guo

et al. 2024

Advanced Science

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The regenerative treatment of infectious vertical bone defects remains difficult and challenging today. Current clinical treatments are limited in their ability to control bacteria and infection, which is unfavorable for new bone formation and calls for a new type of material with excellent osteogenic and antibacterial properties. Here a multifunctional scaffold is synthesized that mimics natural bone nanostructures by incorporating silver nanowires into a hierarchical, intrafibrillar mineralized collagen matrix (IMC/AgNWs), to achieve the therapeutic goals of inhibiting bacterial activity and promoting infectious alveolar bone augmentation in rats and beagle dogs. An appropriate concentration of 0.5 mg mL−1 AgNWs is selected to balance biocompatibility and antibacterial properties. The achieved IMC/AgNWs exhibit a broad spectrum of antimicrobial properties against Gram‐negative Porphyromonas gingivalis and Gram‐positive Streptococcus mutans. When the IMC/AgNWs are cocultured with periodontal ligament stem cells, it possesses excellent osteoinductive activities under both non‐inflammatory and inflammatory conditions. By constructing a rat mandibular infected periodontal defect model, the IMC/AgNWs achieve a near‐complete healing through the canonical BMP/Smad signaling. Moreover, the IMC/AgNWs enhance vertical bone height and osseointegration in peri‐implantitis in beagle dogs, indicating the clinical translational potential of IMC/AgNWs for infectious vertical bone augmentation.

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Suffruticosol A elevates osteoblast differentiation targeting BMP2‐Smad/1/5/8‐RUNX2 in pre‐osteoblasts

Cited by 6 publications

References 67 publications

Vanillin Promotes Osteoblast Differentiation, Mineral Apposition, and Antioxidant Effects in Pre-Osteoblasts

Vanillin Promotes Osteoblast Differentiation, Mineral Apposition, and Antioxidant Effects in Pre-Osteoblasts

Suffruticosol B Is an Osteogenic Inducer through Osteoblast Differentiation, Autophagy, Adhesion, and Migration

A Biomimetic Multifunctional Scaffold for Infectious Vertical Bone Augmentation

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