Sugar-based crosslinker forms a stable atelocollagen hydrogel that is a favorable microenvironment for 3D cell culture

Kamimura, Wataru; Koyama, Hiroyuki; Miyata, Tetsuro; Takato, Tsuyoshi

doi:10.1002/jbm.a.35106

Cited by 16 publications

(16 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are chemical, physical and biological methods available to improve collagen properties. Chemical crosslinking is mostly accomplished by glutaraldehyde [ 13 , 14 , 15 ], ethyl-3(3-dimethylamino) propyl carbodiimide/ N-hydroxysuccinimide (EDC/NHS) [ 11 , 16 , 17 ], diisocyanates [ 18 , 19 ] or oxidized sugar [ 20 , 21 ]. However, chemical crosslinking is often time consuming due to the required wash steps, potentially leaves toxic residues and may reduce biocompatibility [ 18 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Rose Bengal Crosslinking to Stabilize Collagen Sheets and Generate Modulated Collagen Laminates

Eckes

Braun

Wack

et al. 2020

IJMS

View full text Add to dashboard Cite

For medical application, easily accessible biomaterials with tailored properties are desirable. Collagen type I represents a biomaterial of choice for regenerative medicine and tissue engineering. Here, we present a simple method to modify the properties of collagen and to generate collagen laminates. We selected three commercially available collagen sheets with different thicknesses and densities and examined the effect of rose bengal and green light collagen crosslinking (RGX) on properties such as microstructure, swelling degree, mechanical stability, cell compatibility and drug release. The highest impact of RGX was measured for Atelocollagen, for which the swelling degree was reduced from 630% (w/w) to 520% (w/w) and thickness measured under force application increased from 0.014 mm to 0.455 mm, indicating a significant increase in mechanical stability. Microstructural analysis revealed that the sponge-like structure was replaced by a fibrous structure. While the initial burst effect during vancomycin release was not influenced by crosslinking, RGX increased cell proliferation on sheets of Atelocollagen and on Collagen Solutions. We furthermore demonstrate that RGX can be used to covalently attach different sheets to create materials with combined properties, making the modification and combination of readily available sheets with RGX an attractive approach for clinical application.

show abstract

Section: Introductionmentioning

confidence: 99%

Rose Bengal Crosslinking to Stabilize Collagen Sheets and Generate Modulated Collagen Laminates

Eckes

Braun

Wack

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…The molecular size of oxidized oligosaccharides depends on the size of their precursors, so mono- and oligosaccharide-derived ones are reasonably small and could access the deep part of protein hydrogel by free diffusion from a heterogeneous phase. However, to the best of our knowledge, so far this type of compound has only been used in highly dispersed systems by premixing, such as solutions of chitosan [ 12 ], corn starch [ 13 ], atelocollagen [ 14 ] and keratin [ 15 ], but no study on a heterogeneous system has been reported. Furthermore, compared with normal polyaldehydes, oxidized oligosaccharides bear abundant hydroxyl groups which provide higher water solubility and biocompatibility [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Oxidized Oligosaccharides Stabilize Rehydrated Sea Cucumbers against High-Temperature Impact

Liu¹,

Xu²,

Xia³

et al. 2020

IJMS

View full text Add to dashboard Cite

Small-molecule crosslinkers could diffuse into and stabilize protein hydrogels without damaging their appearance, but they are absent from the food industry due to the high safety and efficacy requirements for foods. Oxidized oligosaccharides are non-toxic small polyaldehydes previously found capable of crosslinking proteins by premixing. In this study, we managed to diffuse various oxidized oligosaccharides into the protein wall of rehydrated sea cucumbers, and the texture profile analysis, total soluble material assay and SEM (scanning electron microscope) images all suggested the treated sea cucumbers acquired significantly enhanced stability against high-temperature-promoted deterioration. The stabilization was positively correlated with the aldehyde content of oxidized oligosaccharides but negatively correlated with molecular size. The mechanism of stabilization was found to include both covalent and hydrogen bond crosslinking. These results have demonstrated that oxidized oligosaccharides could enter food protein hydrogel by free diffusion and stabilize the 3D network effectively and thereby has great potential in food-related industry.

show abstract

“…Alternatively, click reactions, formed by the mixing of click group‐modified ECM solutions, can rapidly form drug depots with tunable mechanical properties for in vivo applications . In recent years, click reactions (e.g., copper‐catalyzed azide–alkyne cycloaddition or photoclick reactions) have been used to rapidly form ECM hydrogel depots at physiologically relevant conditions .…”

Section: Introductionmentioning

confidence: 95%

An Injectable, Click‐Cross‐Linked Small Intestinal Submucosa Drug Depot for the Treatment of Rheumatoid Arthritis

Kim

Park

et al. 2016

Adv Healthcare Materials

View full text Add to dashboard Cite

Here, a click-cross-linked small intestine submucosa (SIS) drug depot is described for the treatment of rheumatoid arthritis (RA). To the best of the knowledge, there have been no studies related to the intra-articular injection of methotrexate (Met)-loaded click-cross-linkable SIS (Met-loaded Cx-SIS) for RA treatment. As the key objective of this work, injectable formulations of tetrazine-modified SIS (TE-SIS) and transcyclooctene-modified SIS (TC-SIS) are employed as drug depots. Within a few seconds, the simple mixing of equal amounts of TE-SIS and TC-SIS suspensions forms a gelatinous click-cross-linked SIS (Cx-SIS) drug depot in vitro and in vivo. The formed Cx-SIS depot is maintained in the articular joint over an extended period, while SIS alone rapidly disappears. Injectable formulations of Met-loaded Cx-SIS and Met-loaded SIS are prepared and then injected into articular joints to form drug depots. Compared to animals treated with Met-loaded SIS, RA animals treated with Met-loaded Cx-SIS show effective RA repair, as well as extensive regeneration of chondrocytes and glycosaminoglycan deposits. Collectively, these results indicate that the Met-loaded Cx-SIS depot is successfully formed after intra-articular injection of click-cross-linkable SIS, and that this formulation induces long-lasting Met release and allows Met to act effectively in the articular joint, resulting in RA repair.

show abstract

Sugar-based crosslinker forms a stable atelocollagen hydrogel that is a favorable microenvironment for 3D cell culture

Cited by 16 publications

References 19 publications

Rose Bengal Crosslinking to Stabilize Collagen Sheets and Generate Modulated Collagen Laminates

Rose Bengal Crosslinking to Stabilize Collagen Sheets and Generate Modulated Collagen Laminates

Oxidized Oligosaccharides Stabilize Rehydrated Sea Cucumbers against High-Temperature Impact

An Injectable, Click‐Cross‐Linked Small Intestinal Submucosa Drug Depot for the Treatment of Rheumatoid Arthritis

Contact Info

Product

Resources

About