Sugar-binding sites with specificity for glucosamine in the guinea pig middle ear mucosa

Tanimura, Fumiko; Morioka, Hiroyuki; Murakami, Yasushi

doi:10.1007/bf00180388

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…It is well recognized that cell free endotoxin is significantly more biologically functional than cell bound endotoxin and antibiotics, particularly those that act as inhibitors of cell wall biosynthesis, induce enormous amount of endotoxin release during treatment [7]. Plenty of experimental evidences from in vitro, in vivo and ex vivo models have advocated that antibiotics vary in their ability to trigger endotoxin release from gram-negative microbes [7], [8], [9]. Further, ex vivo evaluation of whole mouse blood has established that there is a correlation between amount of endotoxin release following antibiotic exposure and pro-inflammatory cytokine production [7].…”

Section: Introductionmentioning

confidence: 99%

Zingerone Suppresses Liver Inflammation Induced by Antibiotic Mediated Endotoxemia through Down Regulating Hepatic mRNA Expression of Inflammatory Markers in Pseudomonas aeruginosa Peritonitis Mouse Model

2014

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Antibiotic-induced endotoxin release is associated with high mortality rate even when appropriate antibiotics are used for the treatment of severe infections in intensive care units. Since liver is involved in systemic clearance and detoxification of endotoxin hence it becomes a primary target organ for endotoxin mediated inflammation. Currently available anti-inflammatory drugs give rise to serious side effects. Hence, there is an urgent need for safe and effective anti-inflammatory therapy. It is likely that anti-inflammatory phytochemicals and neutraceutical agents may have the potential to reduce the endotoxin mediated inflammation and complications associated with endotoxin release. Keeping this in mind, the present study was planned to evaluate the hepatoprotective potential of zingerone (active compound of zingiber officinale) against liver inflammation induced by antibiotic mediated endotoxemia. The selected antibiotics capable of releasing high content of endotoxin were employed for their in vivo efficacy in P.aeruginosa peritonitis model. Released endotoxin induced inflammation and zingerone as co-anti-inflammatory therapy significantly reduced inflammatory response. Improved liver histology and reduced inflammatory markers MDA, RNI, MPO, tissue damage markers (AST, ALT, ALP) and inflammatory cytokines (MIP-2, IL-6 and TNF-α) were indicative of therapeutic potential of zingerone. The mechanism of action of zingerone may be related to significant inhibition of the mRNA expression of inflammatory markers (TLR4, RelA, NF-kB2, TNF- α, iNOS, COX-2) indicating that zingerone interferes with cell signalling pathway and suppresses hyper expression of cell signaling molecules of inflammatory pathway. Zingerone therapy significantly protected liver from endotoxin induced inflammatory damage by down regulating biochemical as well as molecular markers of inflammation. In conclusion, this study provides evidence that zingerone is a potent anti-inflammatory phytomedicine against hepatic inflammation induced by antibiotic mediated endotoxemia. These results thus suggest that zingerone treatment can be used as a co-therapy with antibiotics to reduced endotoxin induced inflammation during treatment of severe P.aeruginosa infections.

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Section: Introductionmentioning

confidence: 99%

Zingerone Suppresses Liver Inflammation Induced by Antibiotic Mediated Endotoxemia through Down Regulating Hepatic mRNA Expression of Inflammatory Markers in Pseudomonas aeruginosa Peritonitis Mouse Model

2014

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“…MS chromatogram Indicated absence of many chemical compounds in the zingerone treated LPS as compared to non-treated LPS. Most importantly, glucosamine present in non-treated LPS which serves as a backbone for LPS structure and significantly contributes towards immunostimulatory activity of lipid A (Tanimura et al, 1993). Workers have developed and validated a sensitive HPLC-MS method for the determination of glucosamine in the range of 162.1 m/z to 180.1 m/z ratio (Islan et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Structural alterations in Pseudomonas aeruginosa by zingerone contribute to enhanced susceptibility to antibiotics, serum and phagocytes

2014

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Sugar-binding sites with specificity for glucosamine in the guinea pig middle ear mucosa

Cited by 2 publications

References 23 publications

Zingerone Suppresses Liver Inflammation Induced by Antibiotic Mediated Endotoxemia through Down Regulating Hepatic mRNA Expression of Inflammatory Markers in Pseudomonas aeruginosa Peritonitis Mouse Model

Zingerone Suppresses Liver Inflammation Induced by Antibiotic Mediated Endotoxemia through Down Regulating Hepatic mRNA Expression of Inflammatory Markers in Pseudomonas aeruginosa Peritonitis Mouse Model

Structural alterations in Pseudomonas aeruginosa by zingerone contribute to enhanced susceptibility to antibiotics, serum and phagocytes

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