Diversity of vine varieties is confirmed by different morphological and biochemical characteristics of each variety, especially in bunch and berry properties. The evaluation of mentioned characteristics supplements the chemotaxonomical classification and preservation of local and less known vine varieties. Morphometrical and biochemical characteristics were determined on grape berries of 14 different vine varieties, called 'Barbera', 'Merlot', 'Cabernet sauvignon', 'Syrah', 'Refošk', 'Sladki teran', 'Teran Istra', 'Pokalca', 'Plavina', 'Plovdina', 'Sladkočrn', 'Tinta Pinheira', 'Vranac' in 'Zweigelt', grown in Amphelographic vineyard. Morphological parameters were determined by morphometry, O.I.V. descriptors number codes 220, 221, 503 and with colorimeter, but biochemical parameters with several carbohydrates by HPLC and total acidity with titration. The obtained results confirmed some similarity among selected varieties, and multivariable analyses according to determined parameters group the varieties into 4 groups; in group I varieties 'Barbera Bovcon', 'Pokalca' and 'Barbera standard'; group II varieties 'Refošk', 'Syrah' and 'Teran Istra'; group III varieties 'Cabernet sauvignon', 'Merlot' and 'Zweigelt'; group IV varieties 'Sladkočrn', 'Tinta Pinheira' and 'Vranac'. The variety 'Plovdina' is not included in any group, what confirms its vast variability compared with other varieties taken into account.