Sugar Consumption in Vitro of the Muscle Tissue of Diabetic Patients

I, Magyar; Lehoczky, D; Marton, I

doi:10.2337/diab.14.11.716

Cited by 9 publications

(3 citation statements)

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“…This observation is different from that for muscle of animals made diabetic by anti-insulin serum (GRI~GO]a et al [22]), by alloxan (I~II~ and Co~I [31] and HALL [26] or pancreateetomy (MIGLIO~-I~E and C~AIKOFF [36] and ]~EATTE [1], since the muscles of these animals utilized glucose at a decreased rate. The observation that glucose utilization by muscle from diabetic Chinese hamsters was normal is in agreement with the observation of MAGYA~ [33] that muscle tissue from mild human diabetics took up normal amounts of glucose in vitro. A fundamental difference therefore exists in muscle tissue from diabetic hamsters and man compared with muscle from animals made diabetic through insulin deficiency.…”

Section: Discussionsupporting

confidence: 90%

Characterization of diabetes in the Chinese hamster

Gerritsen

Dulin

1967

Diabetologia

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Diabetes in Chinese hamster was initially detected by qualitative tests for urine ~lucose. The disease was ct~aracterized by quantitating urine glucose, glucose tolerance tests and measurement of fasting and nonfasting blood sugar, blood ketones, plasma free fatty acids (FFA), plasma insulin, pancreatic insulin and fasting levels of liver glycogen. In addition, basal levels of glucose utilization by diaphragms and epididymal adipose tissue and the response of these tissues to insulin was measured. Those animals requiring insulin received their last injection 24 hours prior to study. Glucosuria varied from 51 to 1600 mg/24 hours. Diabetics had significantly decreased tolerance to a 250 mg/kg glucose load. The response varied considerably in diabetics but was uniform in the nondiabetics. Diabetics had mean fasting liver glycogen levels of 3.1 :~ 1.0 compared with 0.4 --0.7 percent of fresh liver weight for nondiabetics. Severely ketotic, diabetic Chinese hamsters had significantly elevated FFA and ketone levels and significantly lower plasma and pancreatic insulin levels but mild diabetics did not differ from controls with respect to these parameters. Alt Chinese hamsters had high plasma FFA levels (nondiabeties 1800 teE/l, severe diabetics 2800 teE/l). Fasting and nonfasting FFA levels did not differ in mild diabetic and nondiabetie animals. Muscle and adipose tissues from diabetic hamsters had basal rates similar to nondiabetics and had similar responses to insulin. Hamsters maintained on insulin had greatly elevated immunoreaetive insulin levels in their plasma, which persisted for 26 days. --The data suggest that severely diabetic hamsters may have a decreased ability of the pancreas to secrete insulin in response to a glucose stimulus. The observations that plasma insulin levels are normal in mild diabetics but that these animals have glucosuria, hyperglycemia and abnormal glucose tolerance suggest that mild diabetes in the Chinese hamster may involve interference with insulin action and/or increased hepatic glucose output.Caract~risation du diab~te du hamster chino@. Vol. 3, No. 2, 1967 G.C. GERaITS]~ and W.E. DuLI~<: Diabetes in the Chinese Hamster 75 ist noch 26 Tage nach der letzten Injektion nachweisbar. --Diese Ergebnisse lessen vermuten, dal~ dcr Pankreas des diabetischen Hamsters nur schwerlich in der Late ist, naeh ether Glucosestimulierung Insulin auszuschiitten. Die Beobachtung, dal~ der Plasma-Insulinspiegel im leithten Diabetes normal ist, diese Tiere jedoch Glucosurie, Hyperglyk~mie and abnormale Glucosetoleranz aufweisen, fiihrt zu der Annahme, dal3 ein leichter Diabetes beim chinesisehen Hamster eine Beeintr~ichtigung der Insulinwirkung mit sich bringen, und Ursache einer vermehrten Glucoseproduktion durch die Leber sein kalm.

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Section: Discussionsupporting

confidence: 90%

Characterization of diabetes in the Chinese hamster

Gerritsen

Dulin

1967

Diabetologia

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“…In recent years a number of papers have described studies on glucose metabolism carried out on preparations of human isolated skeletal muscle (Magyar, Lehoczky & Marton, 1965;Holm & Schersten, 1972;Frayn, Adnitt & Turner, 1973;Nuttall, Barbosa & Gannon, 1974;Suominen, Forsberg, Heikkinen & Osterback, 1974), and it seems likely that similar preparations will be used increasingly in the future for both biochemical and pharmacological investigations. However, little attention has been paid to possible variations in the response due to factors such as the anaesthetic agents used and the sex, age and weight of the patient from whom the muscle sample has been obtained.…”

Section: Introductionmentioning

confidence: 99%

The influence of premedication, anaesthesia, age and weight on glucose uptake into human isolated skeletal muscle.

Kirby¹,

Leighton²,

Turner³

1976

Brit J Clinical Pharma

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1The effect of the anaesthetic procedures and of the sex, age and weight of each patient on glucose uptake and glycogen content of human skeletal muscle has been studied in vitro in the presence and absence of insulin. 2 Statistical analysis indicated that the relationships between age and both glucose uptake and the response to insulin were significant, older patients in general having higher uptakes.3 The glucose uptake was highly correlated with the three obesity indices (ponderal index, body mass index and percentage of the ideal weight). 4 The anaesthetic agents had no significant effect on glucose uptake. 5 The choice of premedication appeared to have a small effect on the basal glucose uptake level, but as the choice of premedication was also age related and age itself was a significant factor, this effect may not be of importance. 6 It is concluded that the age and the degree of obesity of the patients ought to be taken into account when studying samples of human muscle.

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“…Hence, preparation for in vitro studies giving reliable information arc required. Many carefully studied and character ized in vitro preparations of muscle tissue from experimental animals have been presented [1,2,5,7,15,21], On the other hand only a few metabolic studies involving in vitro preparations of human skeletal mus cle tissue have been presented [9,19,26] and so far no detailed evalua tion of the teased human skeletal muscle fibre preparation has appeared.…”

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confidence: 99%

Metabolic Studies in Human Skeletal Muscle Tissue

Lundholm¹,

Bylund²,

Holm³

et al. 1975

Eur Surg Res

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An in vitro preparation of human skeletal muscle tissue aimed to be a model for clinical metabolic studies was functionally and structurally characterized. Muscle fibres from biopsy specimens obtained at surgical operations were teased away and collected in incubation vessels for the following determinations: exchangeable water content, extracellular space, potassium content, incorporation rate of labeled leucine into proteins, uptake of labeled cycloleucine, α-aminoisobutyric acid (AIB), and 3-O-methyl-glucose into the intracellular space, and the incorporation rate of glucose into various metabolites. After various times of incubation, muscle fibre samples were also taken for light and electron microscopy.During the incubation of the muscle fibres a transient intracellular oedema was demonstrated by electron microscopy and by determination of the exchangeable water content. The restitution of this oedema was associated with an increasing intracellular potassium concentration. Active transport of leucine, cycloleucine and AIB showing competitive inhibition was registered. The incorporation of labeled leucine into proteins was linear during 4 h of incubation and optimal stimulation of this incorporation was demonstrated in the presence of a complete amino acids mixture at a concentration corresponding to 10 times the normal plasma concentration in man. Insulin stimulated the incorporation rate of glucose-carbon into all metabolites and ouabain stimulated its incorporation into glycogen. Monoiodoacetate inhibited the incorporation of glucose into all metabolites.The teased human skeletal muscle tissue seems to be a useful model for clinical studies of human skeletal muscle metabolism. Several regulatory systems, which arefunctional in the intact and the cut rat diaphragm were present and functional in these isolated human fibres.

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Sugar Consumption in Vitro of the Muscle Tissue of Diabetic Patients

Cited by 9 publications

References 0 publications

Characterization of diabetes in the Chinese hamster

Characterization of diabetes in the Chinese hamster

The influence of premedication, anaesthesia, age and weight on glucose uptake into human isolated skeletal muscle.

Metabolic Studies in Human Skeletal Muscle Tissue

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