Sugar Flotation Method for Sorting the Resting Eggs of Marine Cladocerans and Copepods from Sea-Bottom Sediment

First evidences of <i>Acartia bifilosa</i> resting eggs in sediments of the Urdaibai estuary (Bay of Biscay): abundance and hatching success

“…These slices were divided into two halves; one for counting eggs and the other for hatching success analysis. Eggs were removed from sediment by the sugar flotation method introduced by Onbé (1978) and modified by Marcus (1990). The eggs for counting were preserved in 2% buffered formalin and analysed using an inverted Olympus microscope at a magnification of 400x.…”

“…Eggs for hatching were extracted in a similar way to eggs for counting, since neither sonication (Marcus, 1984a) nor centrifugation (Onbé, 1978) should affect the eggs' viability. After centrifugation and washing, the eggs of each slice were placed with a Pasteur pipette onto 1 ml 24-well microtitration plates (range: 6-39 eggs per well) with GF/C filtered and autoclaved estuarine water.…”

Unexpected increases in rotifer resting egg abundances during the period of contamination of Lake Orta

“…A sugar flotation technique to extract Rotifer Resting Eggs (RREs) (Onbé, 1978, modified by e.g., Garcia…”

Fluctuations of the population of Daphnia laevis Birge 1878: a six-year study in a tropical lake

“…All the collected material was placed in dark refrigerated flasks (approximately 5 °C) until processing. To separate the eggs from the sediment, the samples were homogenized in a sugar-distilled water solution (1:1) (Onbé, 1978;Marcus, 1990) and centrifuged at 3,600 rpm for 5 minutes. The material retained on the net was washed with distilled water and kept refrigerated (5 °C) in labeled flasks (Crispim and Watanabe, 2000;Maia-Barbosa et al, 2003b).…”