Sugar transport in <i>Sulfolobus solfataricus</i> is mediated by two families of binding protein‐dependent ABC transporters

Elferink, Marieke G. L.; Albers, Sonja‐Verena; Konings, Wil N.; Driessen, Arnold J. M.

doi:10.1046/j.1365-2958.2001.02336.x

Cited by 123 publications

(155 citation statements)

References 36 publications

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“…High-affinity uptake via ABC-type transporters is also common: 11 operons encode a membrane-anchored extracytoplasmic-binding protein, one or two membrane-embedded permeases, and one or two cytoplasmic ATPases (ref. 17; Fig. 1).…”

Section: Physiology and Growthmentioning

confidence: 94%

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The complete genome of the crenarchaeon Sulfolobus solfataricus P2

She

Singh

Confalonieri

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

725

627

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The genome of the crenarchaeon Sulfolobus solfataricus P2 contains 2,992,245 bp on a single chromosome and encodes 2,977 proteins and many RNAs. One-third of the encoded proteins have no detectable homologs in other sequenced genomes. Moreover, 40% appear to be archaeal-specific, and only 12% and 2.3% are shared exclusively with bacteria and eukarya, respectively. The genome shows a high level of plasticity with 200 diverse insertion sequence elements, many putative nonautonomous mobile elements, and evidence of integrase-mediated insertion events. There are also long clusters of regularly spaced tandem repeats. Different transfer systems are used for the uptake of inorganic and organic solutes, and a wealth of intracellular and extracellular proteases, sugar, and sulfur metabolizing enzymes are encoded, as well as enzymes of the central metabolic pathways and motility proteins. The major metabolic electron carrier is not NADH as in bacteria and eukarya but probably ferredoxin. The essential components required for DNA replication, DNA repair and recombination, the cell cycle, transcriptional initiation and translation, but not DNA folding, show a strong eukaryal character with many archaeal-specific features. The results illustrate major differences between crenarchaea and euryarchaea, especially for their DNA replication mechanism and cell cycle processes and their translational apparatus.

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Section: Physiology and Growthmentioning

confidence: 94%

“…Eleven operons encoding ABC transport systems are present, and those with established substrate specificity are depicted (xyl, xylose; fruc, fructose; glu, glucose; gal, galactose; man, mannose. DP, degree of polymerization) (17). At least 15 secondary transporters (permeases) are present (symport and antiport).…”

Section: Physiology and Growthmentioning

confidence: 99%

The complete genome of the crenarchaeon Sulfolobus solfataricus P2

She

Singh

Confalonieri

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

725

627

View full text Add to dashboard Cite

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“…Considering the particular lipid composition of archaea membranes (34,35), it could be hypothesized that thermophilic membrane proteins should have particular functional and structural characteristics. However, although some A1A0-ATPases (the archaeal homologs of F 1 F 0 -ATPases), redox, and electron transport components from archaea have been studied (36,37), only sugar transporter ABC ATPases from Thermococcus litoralis (38,39) and Sulfolobus acidocaldarius (40) have been initially characterized. Thus, little information is available on the structure and function of thermophilic solute transport proteins.…”

Section: P-type Atpases Transport a Variety Of Ions (Hmentioning

confidence: 99%

Characterization of a Thermophilic P-type Ag+/Cu+-ATPase from the ExtremophileArchaeoglobus fulgidus

Mandal

Cheung²,

Argüello

2002

Journal of Biological Chemistry

123

198

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The thermophilic, sulfur metabolizing Archaeoglobus fulgidus contains two genes, AF0473 and AF0152, encoding for PIB-type heavy metal transport ATPases. In this study, we describe the cloning, heterologous expression, purification, and functional characterization of one of these ATPases, CopA (NCB accession number AAB90763), encoded by AF0473. 50 ‫؍‬ 24 M). This is the first Ag ؉ /Cu ؉ -ATPase expressed and purified in a functional form. Thus, it provides a model for structurefunctional studies of these transporters. Moreover, its characterization will also contribute to an understanding of thermophilic ion transporters.

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“…In fact, the occurrence of PTS in thermophiles and hyperthermophiles is extremely rare; the only exception is the thermophilic bacterium Bacillus stearothermophilus, which possesses PTSs for mannitol and cellobiose (8,12). On the other hand, all thermophilic and hyperthermophilic organisms examined thus far transport trehalose via ABC transport systems (5,9,11,18,24,25). The strong reduction in trehalose transport induced by arsenate in R. marinus is consistent with this type of system.…”

Section: Discussionmentioning

confidence: 69%

“…In mesophilic bacteria, like Escherichia coli, the uptake of trehalose is typically mediated by the phosphoenolpyruvate (PEP):sugar phosphotransferase system (PTS) (2). In contrast, the PTS has not been found in extreme thermophiles or hyperthermophiles, and the ATP-binding cassette (ABC) transport system is used for trehalose uptake in the few cases examined thus far (5,9,18,24,25). The high affinity of the ABC transporters seems to be crucial for utilization of the scarce carbon sources typical of the hostile environments where thermophiles and hyperthermophiles are found.…”

Section: Trehalose [␣-D-glucopyranosyl-␣(11)-d-glucopyranoside] Ismentioning

confidence: 99%

Role of Periplasmic Trehalase in Uptake of Trehalose by the Thermophilic Bacterium Rhodothermus marinus

et al. 2008

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Trehalose uptake at 65°C in Rhodothermus marinus was characterized. The profile of trehalose uptake as a function of concentration showed two distinct types of saturation kinetics, and the analysis of the data was complicated by the activity of a periplasmic trehalase. The kinetic parameters of this enzyme determined in whole cells were as follows: K m ‫؍‬ 156 ؎ 11 M and V max ‫؍‬ 21.2 ؎ 0.4 nmol/min/mg of total protein. Therefore, trehalose could be acted upon by this periplasmic activity, yielding glucose that subsequently entered the cell via the glucose uptake system, which was also characterized. To distinguish the several contributions in this intricate system, a mathematical model was developed that took into account the experimental kinetic parameters for trehalase, trehalose transport, glucose transport, competition data with trehalose, glucose, and palatinose, and measurements of glucose diffusion out of the periplasm. It was concluded that R. marinus has distinct transport systems for trehalose and glucose; moreover, the experimental data fit perfectly with a model considering a high-affinity, low-capacity transport system for trehalose (K m ‫؍‬ 0.11 ؎ 0.03 M and V max ‫؍‬ 0.39 ؎ 0.02 nmol/min/mg of protein) and a glucose transporter with moderate affinity and capacity (K m ‫؍‬ 46 ؎ 3 M and V max ‫؍‬ 48 ؎ 1 nmol/min/mg of protein). The contribution of the trehalose transporter is important only in trehalose-poor environments (trehalose concentrations up to 6 M); at higher concentrations trehalose is assimilated primarily via trehalase and the glucose transport system. Trehalose uptake was constitutive, but the activity decreased 60% in response to osmotic stress. The nature of the trehalose transporter and the physiological relevance of these findings are discussed.is a nonreducing disaccharide that is widespread in the three domains of life. This sugar often accumulates intracellularly and protects against a variety of stresses (4, 17). Given the ubiquitous distribution of trehalose in all types of biotopes, it is not surprising that efficient systems for uptake of this sugar evolved in many organisms. In particular, several mechanisms have been discovered in prokaryotes and studied to different extents. In mesophilic bacteria, like Escherichia coli, the uptake of trehalose is typically mediated by the phosphoenolpyruvate (PEP):sugar phosphotransferase system (PTS) (2). In contrast, the PTS has not been found in extreme thermophiles or hyperthermophiles, and the ATP-binding cassette (ABC) transport system is used for trehalose uptake in the few cases examined thus far (5, 9, 18, 24, 25). The high affinity of the ABC transporters seems to be crucial for utilization of the scarce carbon sources typical of the hostile environments where thermophiles and hyperthermophiles are found.Rhodothermus marinus is a thermophilic bacterium with an optimal growth temperature of 65°C, and it was isolated from marine hot springs in the Azores Islands (1). In response to heat or osmotic stress, R. marinus accumulates ...

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Sugar transport in Sulfolobus solfataricus is mediated by two families of binding protein‐dependent ABC transporters

Cited by 123 publications

References 36 publications

The complete genome of the crenarchaeon Sulfolobus solfataricus P2

The complete genome of the crenarchaeon Sulfolobus solfataricus P2

Characterization of a Thermophilic P-type Ag+/Cu+-ATPase from the ExtremophileArchaeoglobus fulgidus

Role of Periplasmic Trehalase in Uptake of Trehalose by the Thermophilic Bacterium Rhodothermus marinus

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