Sugar transporter genes of the brown planthopper, Nilaparvata lugens: A facilitated glucose/fructose transporter

Kikuta, Shingo; Kikawada, Takahiro; Hagiwara‐Komoda, Yuka; Nakashima, Nobuhiko; Noda, Hiroaki

doi:10.1016/j.ibmb.2010.07.008

Cited by 39 publications

(48 citation statements)

References 37 publications

(49 reference statements)

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“…The Nlst16 gene was activated under high sugar conditions, which was not observed in case of the Nlst1 glucose transporter gene. The characteristics of sugar transporters expressed in the gut have been examined in phloem feeding insects such as BPH or aphids, where they have been found to mediate passive sugar incorporations across the gut membranes (Price et al, 2007b(Price et al, , 2010Kikuta et al, 2010;Price and Gatehouse, 2014). The phloem sap entered the gut through continuous BPH feeding, resulting in a sugar-rich environment of the midgut lumen cavity.…”

Section: Discussionmentioning

confidence: 99%

“…Underlines represent EcoRV and EcoRI restriction sites in the forward and reverse primer, respectively. Specific primers of AcGFP1 for a negative control were described in Kikuta et al (2010). The PCR products were cloned into pT7XbG2 (NCBI accession number, AB255037) vectors after digested with each restriction enzyme, EcoRV and EcoRI.…”

Section: Construction Of Nlst16 Crnamentioning

confidence: 99%

“…NlST16 transport activity was analyzed in the Xenopus oocyte expression system as described previously (Kikawada et al, 2007;Kikuta et al, 2010). Briefly described, Nlst16 cRNA was injected into X. laevis oocytes at V or VI stages, and the oocytes were incubated in modified Barth's saline (MBS) buffer for 3 d at 20 C. The MBS buffer composition was described in Kanamori et al (2010).…”

Section: Functional Analyses Of Nlst16 Using Xenopus Oocytesmentioning

confidence: 99%

“…Glucose and fructose, monosaccharides produced from sucrose through hydrolyzation by sucrase (a-glucosidases, EC 3.2.1.20), appear to be incorporated into the body across sugar transporters in the midgut (Price et al, 2007a, b). The glucose transporter gene (NlHT1/Nlst1) and fructose transporter gene (Nlst6) expressed in the apical and basal membranes of the gut in BPH are responsible for monosaccharide transport into the body (Price et al, 2007b;Kikuta et al, 2010). These sugar transporters, typically characterized as facilitative in the solute carrier 2 (SLC2) family, possess 12 transmembrane regions, and transport sugars along gradients from higher to lower concentrations (Wood and Trayhurn, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Our previous study showed that at least 7 Nlst gene members, including Nlst1 and Nlst6, were expressed in the midgut . The characteristics of NlST1 and NlST6 transport activity have been revealed using exogenous expression analyses on Pichia or Xenopus oocytes (Price et al, 2007b;Kikuta et al, 2010), whereas those of 5 other putative transporter genes expressed in the midgut are yet to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Herbivory-induced glucose transporter gene expression in the brown planthopper, Nilaparvata lugens

Kikuta

Nakamura

Hattori

et al. 2015

Insect Biochemistry and Molecular Biology

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Section: Discussionmentioning

confidence: 99%

Section: Construction Of Nlst16 Crnamentioning

confidence: 99%

Section: Functional Analyses Of Nlst16 Using Xenopus Oocytesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Herbivory-induced glucose transporter gene expression in the brown planthopper, Nilaparvata lugens

Kikuta

Nakamura

Hattori

et al. 2015

Insect Biochemistry and Molecular Biology

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Proton gradient mediates hemolymph trehalose influx into aphid bacteriocytes

Iwata

Yoshinaga

Mizutani

et al. 2022

Arch Insect Biochem Physiol

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Aphids harbor proteobacterial endosymbionts such as Buchnera aphidicola housed in specialized bacteriocytes derived from host cells. The endosymbiont Buchnera supplies essential amino acids such as arginine to the host cells and, in turn, obtains sugars needed for its survival from the hemolymph. The mechanism of sugar supply in aphid bacteriocytes has been rarely studied. It also remains unclear how Buchnera acquires its carbon source. The hemolymph sugars in Acyrthosiphon pisum are composed of the disaccharide trehalose containing two glucose molecules. Here, we report for the first time that trehalose is transported and used as a potential carbon source by Buchnera across the bacteriocyte plasma membrane via trehalose transporters. The current study characterized the bacteriocyte trehalose transporter Ap_ST11 (LOC100159441) using the Xenopus oocyte expression system. The Ap_ST11 transporter was found to be proton‐dependent with a Km value ≥700 mM. We re‐examined the hemolymph trehalose at 217.8 mM using a fluorescent trehalose sensor. The bacteriocytes did not obtain trehalose by facilitated diffusion along the gradient across cellular membranes. These findings suggest that trehalose influx into the bacteriocytes depends on the extracellular proton‐driven secondary electrochemical transporter.

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