Sugar unloading in roots of <i>Ricinus communis</i> L.

Chapleo, S.; Hall, J. L.

doi:10.1111/j.1469-8137.1989.tb00699.x

Cited by 13 publications

(10 citation statements)

References 19 publications

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“…(mol min"'g"' protein). These rates are relatively high compared with literature data for roots of R. communis (3-12 /(mol min"' g"' protein, Chapleo & Hall, 1989), Z. mays (0-6-1 /imol min"' g""' f. wt. or 170-270 /(mol min"'g"' protein.…”

Section: Discussionmentioning

confidence: 52%

Acid invertase in mycorrhizal and non‐mycorrhizal roots of Norway spruce (Picea abies [L.] Karst.) seedlings

et al. 1995

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S U M M A R YSucrolytie enyzme activities and concentrations of hexoses typically increase in plant/microbe symhioses. However, there is little information on ectomycorrhizal associations. We measured invertase activity and soluble sugar contents in roots of mycorrhizal and non-tnycorrhizal Norway spruce {Picea abies (L.) Karst.] seedlings. We used two ectomycorrhizal fungi, a basidiomycete [Amanita muscaria (L. ex Fr.) Hooker] and an ascomycete {Cenococcum geophilum Er.). Mycorrhizas and non-mycorrhizal short roots, as well as other parts ofthe root system, were investigated at different developmental stages by micro-analytical methods.Neither sucrose nor invertase could he detected in fungal mycelia. In vitro measurable invertase activity and the sucrose, glucose and fructose content were reduced in both types of myeorrhizas compared with the nonmycorrhizal short roots. Correction of data for the fungal component in the mycorrhizas, indicated that there were no differences in plant-specifie amounts of sucrose and aeid invertase activity in niycorrhizal and non-mycorrhizal roots. However, amounts of glucose and fructose, which are present in both partners, were clearly reduced in the myeorrhizas. As high fructose levels inhibit acid invertase, a reduction in the aniount of fructose in the symbiotic tissue could favour in vivo acid invertase activity. Our results indicate that the situation in eetomyeorrhizas may be different from those in other biotrophic interactions.

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Section: Discussionmentioning

confidence: 52%

Acid invertase in mycorrhizal and non‐mycorrhizal roots of Norway spruce (Picea abies [L.] Karst.) seedlings

et al. 1995

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“…Sucrose uptake was more sensitive to PCMBS than glucose uptake, perhaps due to PCMBS inhibition of extracellular acid invertase (Chapleo & Hall, 1989a).…”

Section: Compartmental Analysis Of Root Tips (A) and Of Whole Tap Rmentioning

confidence: 99%

“…gibsonii with 6-10 cm long tap roots were grown in vermiculite, as described previously (Chapleo & Hall, 1989a). Routinely 10 mm long root tips were used; dissection into cortex, stele and longitudinal segments was also as described previously.…”

Section: Methodsmentioning

confidence: 99%

Sugar unloading in roots of Ricinus communis L.

Chapleo

Hall

1989

New Phytologist

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S U M M A R Y' lie accumulation of exogenous D-fructose, D-glucose atid sucrose into root tips of Ricinus communis was studied. Accumulation of sucrose is biphasic with respect to substrate concentration, both saturable and non-saturable phases being discerned. Accumulation is sensitive to pH, the exogenous solute potential, PCMBS, and other sugars. Sap representative of the apoplast was collected by centrifugation and contains approximately 40 mol Tl -hexose equivalents. Plants were labelled to the steady state with respect to cotyledonary sucrose derivatives; \'hole roots were then analysed by compartmental analysis and the r-esults indicated similar apoplastic hexose concentrations. The vacuoles of roots grown in low salt solution contain most of the root soluble sugars, at a 'concentration of about 70 mol m '' hexose equivalents. The sugar content of such roots decreases with availability "f KCl to an extent which indicates that this involves a replacement of vacuolar hexose by alternative osmotica, 'supporting the results frot-n compartmental analysis.

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“…Peak activity for root invertase is generally 2 to 3 mm behind the apex and corresponds to the region of expansion and elongation in pea (28) and maize (16). In Ricinus, this activity predominates in the cortex (3).…”

Section: Discussionmentioning

confidence: 99%

Organ-Specific Invertase Deficiency in the Primary Root of an Inbred Maize Line

Duke¹,

McCarty²,

Koch³

1991

Plant Physiol.

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An organ-specific invertase deficiency affecting only the primary root system is described in the Oh 43 inbred maize (Zea mays). Invertases (acid and neutral/soluble and insoluble) were assayed in various tissues of hybrid (NK 508) Sucrose breakdown is critical to the vast majority of plant species because nonphotosynthetic tissues depend on import ofthis sugar for their growth and development. Initial cleavage of sucrose can be catalyzed by either invertase (EC 3.2.1.26) or the reversible enzyme sucrose synthase (EC 2.4.1.13). Invertases are especially active in tissues undergoing rapid cell division, such as shoot and root apices (1). Previously, the role of invertases in sucrose transfer was considered particularly important in plants such as sugar cane and maize in which substantial sugar movement occurred through the cell wall space and was accompanied by action of an extracellular invertase (13,15). Recent evidence, however, indicates that, although much hydrolysis is often observed, invertase activity may not be essential for sucrose uptake into either sugar cane stems (21,31) or maize kernels (30).

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Sugar unloading in roots of Ricinus communis L.

Cited by 13 publications

References 19 publications

Acid invertase in mycorrhizal and non‐mycorrhizal roots of Norway spruce (Picea abies [L.] Karst.) seedlings

Acid invertase in mycorrhizal and non‐mycorrhizal roots of Norway spruce (Picea abies [L.] Karst.) seedlings

Sugar unloading in roots of Ricinus communis L.

Organ-Specific Invertase Deficiency in the Primary Root of an Inbred Maize Line

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